Job ID = 14520654 SRX = SRX8244963 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16420610 spots for SRR11684174/SRR11684174.sra Written 16420610 spots for SRR11684174/SRR11684174.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:43 16420610 reads; of these: 16420610 (100.00%) were unpaired; of these: 4003851 (24.38%) aligned 0 times 9736757 (59.30%) aligned exactly 1 time 2680002 (16.32%) aligned >1 times 75.62% overall alignment rate Time searching: 00:01:43 Overall time: 00:01:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5549196 / 12416759 = 0.4469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:26: 1000000 INFO @ Sat, 15 Jan 2022 19:37:31: 2000000 INFO @ Sat, 15 Jan 2022 19:37:35: 3000000 INFO @ Sat, 15 Jan 2022 19:37:40: 4000000 INFO @ Sat, 15 Jan 2022 19:37:44: 5000000 INFO @ Sat, 15 Jan 2022 19:37:49: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:37:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:53: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:37:53: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:37:53: #1 total tags in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:37:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:37:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:37:53: #1 tags after filtering in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:37:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:37:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:37:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:37:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:37:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:37:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:37:57: 1000000 INFO @ Sat, 15 Jan 2022 19:38:03: 2000000 INFO @ Sat, 15 Jan 2022 19:38:08: 3000000 INFO @ Sat, 15 Jan 2022 19:38:14: 4000000 INFO @ Sat, 15 Jan 2022 19:38:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:38:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:38:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:38:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:38:25: 6000000 INFO @ Sat, 15 Jan 2022 19:38:26: 1000000 INFO @ Sat, 15 Jan 2022 19:38:30: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:38:30: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:38:30: #1 total tags in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:38:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:30: #1 tags after filtering in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:38:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:38:30: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:38:31: 2000000 INFO @ Sat, 15 Jan 2022 19:38:36: 3000000 INFO @ Sat, 15 Jan 2022 19:38:41: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:38:46: 5000000 INFO @ Sat, 15 Jan 2022 19:38:51: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:38:55: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 19:38:55: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 19:38:55: #1 total tags in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:38:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:38:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:38:55: #1 tags after filtering in treatment: 6867563 INFO @ Sat, 15 Jan 2022 19:38:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:38:55: #1 finished! INFO @ Sat, 15 Jan 2022 19:38:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:38:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:38:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:38:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:38:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling