Job ID = 10223992 SRX = SRX8244963 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16420610 spots for SRR11684174/SRR11684174.sra Written 16420610 spots for SRR11684174/SRR11684174.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:21 16420610 reads; of these: 16420610 (100.00%) were unpaired; of these: 4003851 (24.38%) aligned 0 times 9736757 (59.30%) aligned exactly 1 time 2680002 (16.32%) aligned >1 times 75.62% overall alignment rate Time searching: 00:01:21 Overall time: 00:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5549196 / 12416759 = 0.4469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:06:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:06:35: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:06:35: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:06:40: 1000000 INFO @ Fri, 16 Oct 2020 09:06:45: 2000000 INFO @ Fri, 16 Oct 2020 09:06:49: 3000000 INFO @ Fri, 16 Oct 2020 09:06:53: 4000000 INFO @ Fri, 16 Oct 2020 09:06:58: 5000000 INFO @ Fri, 16 Oct 2020 09:07:02: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:07:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:07:05: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:07:05: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:07:06: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:07:06: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:07:06: #1 total tags in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:07:06: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:07:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:07:06: #1 tags after filtering in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:07:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:07:06: #1 finished! INFO @ Fri, 16 Oct 2020 09:07:06: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:07:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:07:06: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:07:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:07:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:07:10: 1000000 INFO @ Fri, 16 Oct 2020 09:07:16: 2000000 INFO @ Fri, 16 Oct 2020 09:07:21: 3000000 INFO @ Fri, 16 Oct 2020 09:07:26: 4000000 INFO @ Fri, 16 Oct 2020 09:07:31: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:07:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:07:35: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:07:35: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:07:36: 6000000 INFO @ Fri, 16 Oct 2020 09:07:41: 1000000 INFO @ Fri, 16 Oct 2020 09:07:41: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:07:41: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:07:41: #1 total tags in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:07:41: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:07:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:07:41: #1 tags after filtering in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:07:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:07:41: #1 finished! INFO @ Fri, 16 Oct 2020 09:07:41: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:07:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:07:41: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:07:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:07:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:07:45: 2000000 INFO @ Fri, 16 Oct 2020 09:07:50: 3000000 INFO @ Fri, 16 Oct 2020 09:07:55: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:07:59: 5000000 INFO @ Fri, 16 Oct 2020 09:08:04: 6000000 INFO @ Fri, 16 Oct 2020 09:08:08: #1 tag size is determined as 42 bps INFO @ Fri, 16 Oct 2020 09:08:08: #1 tag size = 42 INFO @ Fri, 16 Oct 2020 09:08:08: #1 total tags in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:08:08: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:08:08: #1 tags after filtering in treatment: 6867563 INFO @ Fri, 16 Oct 2020 09:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:08:08: #1 finished! INFO @ Fri, 16 Oct 2020 09:08:08: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:08:08: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:08:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:08:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8244963/SRX8244963.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。