Job ID = 14520157 SRX = SRX8158111 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7564265 spots for SRR11591127/SRR11591127.sra Written 7564265 spots for SRR11591127/SRR11591127.sra Read 7959014 spots for SRR11591128/SRR11591128.sra Written 7959014 spots for SRR11591128/SRR11591128.sra Read 10770700 spots for SRR11591129/SRR11591129.sra Written 10770700 spots for SRR11591129/SRR11591129.sra Read 6402922 spots for SRR11591130/SRR11591130.sra Written 6402922 spots for SRR11591130/SRR11591130.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:09 32696901 reads; of these: 32696901 (100.00%) were unpaired; of these: 1313196 (4.02%) aligned 0 times 16396720 (50.15%) aligned exactly 1 time 14986985 (45.84%) aligned >1 times 95.98% overall alignment rate Time searching: 00:07:09 Overall time: 00:07:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20889494 / 31383705 = 0.6656 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:46:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:46:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:46:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:47:00: 1000000 INFO @ Sat, 15 Jan 2022 18:47:13: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:47:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:47:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:47:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:47:26: 3000000 INFO @ Sat, 15 Jan 2022 18:47:27: 1000000 INFO @ Sat, 15 Jan 2022 18:47:38: 2000000 INFO @ Sat, 15 Jan 2022 18:47:40: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:47:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:47:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:47:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:47:48: 3000000 INFO @ Sat, 15 Jan 2022 18:47:54: 5000000 INFO @ Sat, 15 Jan 2022 18:47:59: 4000000 INFO @ Sat, 15 Jan 2022 18:48:02: 1000000 INFO @ Sat, 15 Jan 2022 18:48:10: 6000000 INFO @ Sat, 15 Jan 2022 18:48:10: 5000000 INFO @ Sat, 15 Jan 2022 18:48:16: 2000000 INFO @ Sat, 15 Jan 2022 18:48:22: 6000000 INFO @ Sat, 15 Jan 2022 18:48:25: 7000000 INFO @ Sat, 15 Jan 2022 18:48:31: 3000000 INFO @ Sat, 15 Jan 2022 18:48:32: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:48:41: 8000000 INFO @ Sat, 15 Jan 2022 18:48:43: 8000000 INFO @ Sat, 15 Jan 2022 18:48:46: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:48:55: 9000000 INFO @ Sat, 15 Jan 2022 18:48:57: 9000000 INFO @ Sat, 15 Jan 2022 18:49:01: 5000000 INFO @ Sat, 15 Jan 2022 18:49:06: 10000000 INFO @ Sat, 15 Jan 2022 18:49:12: 10000000 INFO @ Sat, 15 Jan 2022 18:49:12: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:49:12: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:49:12: #1 total tags in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:49:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:49:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:49:12: #1 tags after filtering in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:49:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:49:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:49:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:49:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:49:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:49:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:49:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:49:16: 6000000 INFO @ Sat, 15 Jan 2022 18:49:19: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:49:19: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:49:19: #1 total tags in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:49:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:49:19: #1 tags after filtering in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:49:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:49:19: #1 finished! INFO @ Sat, 15 Jan 2022 18:49:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:49:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:49:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:49:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:49:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:49:29: 7000000 INFO @ Sat, 15 Jan 2022 18:49:42: 8000000 INFO @ Sat, 15 Jan 2022 18:49:55: 9000000 INFO @ Sat, 15 Jan 2022 18:50:08: 10000000 INFO @ Sat, 15 Jan 2022 18:50:14: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:50:14: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:50:14: #1 total tags in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:50:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:50:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:50:14: #1 tags after filtering in treatment: 10494211 INFO @ Sat, 15 Jan 2022 18:50:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:50:14: #1 finished! INFO @ Sat, 15 Jan 2022 18:50:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:50:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:50:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:50:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158111/SRX8158111.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling