Job ID = 14520147 SRX = SRX8158107 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7975966 spots for SRR11591121/SRR11591121.sra Written 7975966 spots for SRR11591121/SRR11591121.sra Read 5051970 spots for SRR11591122/SRR11591122.sra Written 5051970 spots for SRR11591122/SRR11591122.sra Read 7865174 spots for SRR11591123/SRR11591123.sra Written 7865174 spots for SRR11591123/SRR11591123.sra Read 4794325 spots for SRR11591124/SRR11591124.sra Written 4794325 spots for SRR11591124/SRR11591124.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:32 25687435 reads; of these: 25687435 (100.00%) were unpaired; of these: 2555518 (9.95%) aligned 0 times 18167230 (70.72%) aligned exactly 1 time 4964687 (19.33%) aligned >1 times 90.05% overall alignment rate Time searching: 00:04:32 Overall time: 00:04:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11857908 / 23131917 = 0.5126 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:37:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:37:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:37:45: 1000000 INFO @ Sat, 15 Jan 2022 18:37:52: 2000000 INFO @ Sat, 15 Jan 2022 18:38:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:07: 4000000 INFO @ Sat, 15 Jan 2022 18:38:15: 1000000 INFO @ Sat, 15 Jan 2022 18:38:16: 5000000 INFO @ Sat, 15 Jan 2022 18:38:24: 6000000 INFO @ Sat, 15 Jan 2022 18:38:24: 2000000 INFO @ Sat, 15 Jan 2022 18:38:32: 7000000 INFO @ Sat, 15 Jan 2022 18:38:32: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:38:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:38:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:38:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:38:41: 8000000 INFO @ Sat, 15 Jan 2022 18:38:41: 4000000 INFO @ Sat, 15 Jan 2022 18:38:45: 1000000 INFO @ Sat, 15 Jan 2022 18:38:49: 9000000 INFO @ Sat, 15 Jan 2022 18:38:49: 5000000 INFO @ Sat, 15 Jan 2022 18:38:52: 2000000 INFO @ Sat, 15 Jan 2022 18:38:57: 10000000 INFO @ Sat, 15 Jan 2022 18:38:58: 6000000 INFO @ Sat, 15 Jan 2022 18:39:00: 3000000 INFO @ Sat, 15 Jan 2022 18:39:06: 11000000 INFO @ Sat, 15 Jan 2022 18:39:07: 7000000 INFO @ Sat, 15 Jan 2022 18:39:08: 4000000 INFO @ Sat, 15 Jan 2022 18:39:08: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:39:08: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:39:08: #1 total tags in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:39:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:08: #1 tags after filtering in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:39:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:39:08: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:39:15: 5000000 INFO @ Sat, 15 Jan 2022 18:39:15: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:39:23: 6000000 INFO @ Sat, 15 Jan 2022 18:39:24: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:39:30: 7000000 INFO @ Sat, 15 Jan 2022 18:39:32: 10000000 INFO @ Sat, 15 Jan 2022 18:39:38: 8000000 INFO @ Sat, 15 Jan 2022 18:39:40: 11000000 INFO @ Sat, 15 Jan 2022 18:39:43: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:39:43: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:39:43: #1 total tags in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:39:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:39:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:39:43: #1 tags after filtering in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:39:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:39:43: #1 finished! INFO @ Sat, 15 Jan 2022 18:39:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:39:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:39:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:39:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:39:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:39:45: 9000000 INFO @ Sat, 15 Jan 2022 18:39:52: 10000000 INFO @ Sat, 15 Jan 2022 18:39:59: 11000000 INFO @ Sat, 15 Jan 2022 18:40:01: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:40:01: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:40:01: #1 total tags in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:40:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:01: #1 tags after filtering in treatment: 11274009 INFO @ Sat, 15 Jan 2022 18:40:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:01: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158107/SRX8158107.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling