Job ID = 14520146 SRX = SRX8158106 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6974648 spots for SRR11591117/SRR11591117.sra Written 6974648 spots for SRR11591117/SRR11591117.sra Read 6141728 spots for SRR11591118/SRR11591118.sra Written 6141728 spots for SRR11591118/SRR11591118.sra Read 6685079 spots for SRR11591119/SRR11591119.sra Written 6685079 spots for SRR11591119/SRR11591119.sra Read 6373003 spots for SRR11591120/SRR11591120.sra Written 6373003 spots for SRR11591120/SRR11591120.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:29 26174458 reads; of these: 26174458 (100.00%) were unpaired; of these: 2398917 (9.17%) aligned 0 times 15640695 (59.76%) aligned exactly 1 time 8134846 (31.08%) aligned >1 times 90.83% overall alignment rate Time searching: 00:05:29 Overall time: 00:05:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13086953 / 23775541 = 0.5504 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:29: 1000000 INFO @ Sat, 15 Jan 2022 18:39:35: 2000000 INFO @ Sat, 15 Jan 2022 18:39:41: 3000000 INFO @ Sat, 15 Jan 2022 18:39:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:39:53: 5000000 INFO @ Sat, 15 Jan 2022 18:39:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:39:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:39:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:39:59: 6000000 INFO @ Sat, 15 Jan 2022 18:40:00: 1000000 INFO @ Sat, 15 Jan 2022 18:40:06: 2000000 INFO @ Sat, 15 Jan 2022 18:40:06: 7000000 INFO @ Sat, 15 Jan 2022 18:40:12: 3000000 INFO @ Sat, 15 Jan 2022 18:40:13: 8000000 INFO @ Sat, 15 Jan 2022 18:40:18: 4000000 INFO @ Sat, 15 Jan 2022 18:40:20: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:40:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:40:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:40:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:40:24: 5000000 INFO @ Sat, 15 Jan 2022 18:40:27: 10000000 INFO @ Sat, 15 Jan 2022 18:40:30: 1000000 INFO @ Sat, 15 Jan 2022 18:40:30: 6000000 INFO @ Sat, 15 Jan 2022 18:40:32: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:40:32: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:40:32: #1 total tags in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:40:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:32: #1 tags after filtering in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:40:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:40:36: 2000000 INFO @ Sat, 15 Jan 2022 18:40:36: 7000000 INFO @ Sat, 15 Jan 2022 18:40:42: 3000000 INFO @ Sat, 15 Jan 2022 18:40:42: 8000000 INFO @ Sat, 15 Jan 2022 18:40:48: 4000000 INFO @ Sat, 15 Jan 2022 18:40:48: 9000000 INFO @ Sat, 15 Jan 2022 18:40:54: 5000000 INFO @ Sat, 15 Jan 2022 18:40:54: 10000000 INFO @ Sat, 15 Jan 2022 18:40:58: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:40:58: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:40:58: #1 total tags in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:40:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:40:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:40:59: #1 tags after filtering in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:40:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:40:59: #1 finished! INFO @ Sat, 15 Jan 2022 18:40:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:40:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:40:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:40:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:41:00: 6000000 INFO @ Sat, 15 Jan 2022 18:41:05: 7000000 INFO @ Sat, 15 Jan 2022 18:41:11: 8000000 INFO @ Sat, 15 Jan 2022 18:41:16: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:41:22: 10000000 INFO @ Sat, 15 Jan 2022 18:41:25: #1 tag size is determined as 75 bps INFO @ Sat, 15 Jan 2022 18:41:25: #1 tag size = 75 INFO @ Sat, 15 Jan 2022 18:41:25: #1 total tags in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:41:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:41:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:41:26: #1 tags after filtering in treatment: 10688588 INFO @ Sat, 15 Jan 2022 18:41:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:41:26: #1 finished! INFO @ Sat, 15 Jan 2022 18:41:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:41:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:41:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:41:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:41:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8158106/SRX8158106.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。