Job ID = 14520702 SRX = SRX8065707 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 14345616 spots for SRR11489730/SRR11489730.sra Written 14345616 spots for SRR11489730/SRR11489730.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:35 14345616 reads; of these: 14345616 (100.00%) were unpaired; of these: 647864 (4.52%) aligned 0 times 11197705 (78.06%) aligned exactly 1 time 2500047 (17.43%) aligned >1 times 95.48% overall alignment rate Time searching: 00:02:35 Overall time: 00:02:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6615996 / 13697752 = 0.4830 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:50:33: 1000000 INFO @ Sat, 15 Jan 2022 19:50:39: 2000000 INFO @ Sat, 15 Jan 2022 19:50:44: 3000000 INFO @ Sat, 15 Jan 2022 19:50:50: 4000000 INFO @ Sat, 15 Jan 2022 19:50:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:50:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:50:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:50:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:01: 6000000 INFO @ Sat, 15 Jan 2022 19:51:02: 1000000 INFO @ Sat, 15 Jan 2022 19:51:07: 7000000 INFO @ Sat, 15 Jan 2022 19:51:07: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:07: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:07: #1 total tags in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:51:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:07: #1 tags after filtering in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:51:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:08: 2000000 INFO @ Sat, 15 Jan 2022 19:51:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:12: 3000000 INFO @ Sat, 15 Jan 2022 19:51:17: 4000000 INFO @ Sat, 15 Jan 2022 19:51:22: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:27: 6000000 INFO @ Sat, 15 Jan 2022 19:51:32: 1000000 INFO @ Sat, 15 Jan 2022 19:51:32: 7000000 INFO @ Sat, 15 Jan 2022 19:51:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:51:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:51:33: #1 total tags in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:51:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:33: #1 tags after filtering in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:51:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:51:33: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:51:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:51:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:51:37: 2000000 INFO @ Sat, 15 Jan 2022 19:51:41: 3000000 INFO @ Sat, 15 Jan 2022 19:51:46: 4000000 INFO @ Sat, 15 Jan 2022 19:51:51: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:51:56: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:52:00: 7000000 INFO @ Sat, 15 Jan 2022 19:52:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:52:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:52:01: #1 total tags in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:52:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:01: #1 tags after filtering in treatment: 7081756 INFO @ Sat, 15 Jan 2022 19:52:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:52:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:52:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065707/SRX8065707.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling