Job ID = 14520701 SRX = SRX8065706 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31950376 spots for SRR11489729/SRR11489729.sra Written 31950376 spots for SRR11489729/SRR11489729.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:04 31950376 reads; of these: 31950376 (100.00%) were unpaired; of these: 18088616 (56.61%) aligned 0 times 10015298 (31.35%) aligned exactly 1 time 3846462 (12.04%) aligned >1 times 43.39% overall alignment rate Time searching: 00:03:04 Overall time: 00:03:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7550133 / 13861760 = 0.5447 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:48:35: 1000000 INFO @ Sat, 15 Jan 2022 19:48:41: 2000000 INFO @ Sat, 15 Jan 2022 19:48:47: 3000000 INFO @ Sat, 15 Jan 2022 19:48:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:48:59: 5000000 INFO @ Sat, 15 Jan 2022 19:48:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:48:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:48:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:06: 6000000 INFO @ Sat, 15 Jan 2022 19:49:06: 1000000 INFO @ Sat, 15 Jan 2022 19:49:08: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:49:08: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:49:08: #1 total tags in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:49:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:08: #1 tags after filtering in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:49:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:12: 2000000 INFO @ Sat, 15 Jan 2022 19:49:18: 3000000 INFO @ Sat, 15 Jan 2022 19:49:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:49:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:49:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:49:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:49:31: 5000000 INFO @ Sat, 15 Jan 2022 19:49:37: 1000000 INFO @ Sat, 15 Jan 2022 19:49:38: 6000000 INFO @ Sat, 15 Jan 2022 19:49:40: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:49:40: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:49:40: #1 total tags in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:49:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:41: #1 tags after filtering in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:49:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:49:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:44: 2000000 INFO @ Sat, 15 Jan 2022 19:49:50: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:49:57: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:50:03: 5000000 INFO @ Sat, 15 Jan 2022 19:50:09: 6000000 INFO @ Sat, 15 Jan 2022 19:50:10: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:50:10: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:50:10: #1 total tags in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:50:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:50:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:50:10: #1 tags after filtering in treatment: 6311627 INFO @ Sat, 15 Jan 2022 19:50:10: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:50:10: #1 finished! INFO @ Sat, 15 Jan 2022 19:50:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:50:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:50:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:50:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065706/SRX8065706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling