Job ID = 14520699 SRX = SRX8065704 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 38024084 spots for SRR11489727/SRR11489727.sra Written 38024084 spots for SRR11489727/SRR11489727.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:52 38024084 reads; of these: 38024084 (100.00%) were unpaired; of these: 10394933 (27.34%) aligned 0 times 18898201 (49.70%) aligned exactly 1 time 8730950 (22.96%) aligned >1 times 72.66% overall alignment rate Time searching: 00:04:52 Overall time: 00:04:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16545898 / 27629151 = 0.5989 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:52: 1000000 INFO @ Sat, 15 Jan 2022 19:52:59: 2000000 INFO @ Sat, 15 Jan 2022 19:53:06: 3000000 INFO @ Sat, 15 Jan 2022 19:53:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:53:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:53:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:53:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:19: 5000000 INFO @ Sat, 15 Jan 2022 19:53:22: 1000000 INFO @ Sat, 15 Jan 2022 19:53:26: 6000000 INFO @ Sat, 15 Jan 2022 19:53:28: 2000000 INFO @ Sat, 15 Jan 2022 19:53:33: 7000000 INFO @ Sat, 15 Jan 2022 19:53:34: 3000000 INFO @ Sat, 15 Jan 2022 19:53:39: 8000000 INFO @ Sat, 15 Jan 2022 19:53:41: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:53:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:53:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:53:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:46: 9000000 INFO @ Sat, 15 Jan 2022 19:53:47: 5000000 INFO @ Sat, 15 Jan 2022 19:53:52: 1000000 INFO @ Sat, 15 Jan 2022 19:53:53: 10000000 INFO @ Sat, 15 Jan 2022 19:53:55: 6000000 INFO @ Sat, 15 Jan 2022 19:53:58: 2000000 INFO @ Sat, 15 Jan 2022 19:54:00: 11000000 INFO @ Sat, 15 Jan 2022 19:54:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:54:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:54:01: #1 total tags in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:01: #1 tags after filtering in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:54:02: 7000000 INFO @ Sat, 15 Jan 2022 19:54:05: 3000000 INFO @ Sat, 15 Jan 2022 19:54:08: 8000000 INFO @ Sat, 15 Jan 2022 19:54:11: 4000000 INFO @ Sat, 15 Jan 2022 19:54:14: 9000000 INFO @ Sat, 15 Jan 2022 19:54:18: 5000000 INFO @ Sat, 15 Jan 2022 19:54:20: 10000000 INFO @ Sat, 15 Jan 2022 19:54:25: 6000000 INFO @ Sat, 15 Jan 2022 19:54:26: 11000000 INFO @ Sat, 15 Jan 2022 19:54:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:54:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:54:27: #1 total tags in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:27: #1 tags after filtering in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:27: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:54:31: 7000000 INFO @ Sat, 15 Jan 2022 19:54:37: 8000000 INFO @ Sat, 15 Jan 2022 19:54:43: 9000000 INFO @ Sat, 15 Jan 2022 19:54:49: 10000000 INFO @ Sat, 15 Jan 2022 19:54:55: 11000000 INFO @ Sat, 15 Jan 2022 19:54:56: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:54:56: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:54:56: #1 total tags in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:54:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:54:56: #1 tags after filtering in treatment: 11083253 INFO @ Sat, 15 Jan 2022 19:54:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:54:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:54:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:54:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:54:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:54:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:54:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065704/SRX8065704.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。