Job ID = 14520695 SRX = SRX8065700 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 37241810 spots for SRR11489723/SRR11489723.sra Written 37241810 spots for SRR11489723/SRR11489723.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:38 37241810 reads; of these: 37241810 (100.00%) were unpaired; of these: 1062349 (2.85%) aligned 0 times 29459980 (79.10%) aligned exactly 1 time 6719481 (18.04%) aligned >1 times 97.15% overall alignment rate Time searching: 00:05:38 Overall time: 00:05:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 19553828 / 36179461 = 0.5405 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:53:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:53:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:53:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:53:45: 1000000 INFO @ Sat, 15 Jan 2022 19:53:50: 2000000 INFO @ Sat, 15 Jan 2022 19:53:55: 3000000 INFO @ Sat, 15 Jan 2022 19:54:00: 4000000 INFO @ Sat, 15 Jan 2022 19:54:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:10: 6000000 INFO @ Sat, 15 Jan 2022 19:54:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:16: 7000000 INFO @ Sat, 15 Jan 2022 19:54:17: 1000000 INFO @ Sat, 15 Jan 2022 19:54:22: 8000000 INFO @ Sat, 15 Jan 2022 19:54:24: 2000000 INFO @ Sat, 15 Jan 2022 19:54:28: 9000000 INFO @ Sat, 15 Jan 2022 19:54:30: 3000000 INFO @ Sat, 15 Jan 2022 19:54:34: 10000000 INFO @ Sat, 15 Jan 2022 19:54:37: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:54:40: 11000000 INFO @ Sat, 15 Jan 2022 19:54:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:54:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:54:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:54:44: 5000000 INFO @ Sat, 15 Jan 2022 19:54:46: 12000000 INFO @ Sat, 15 Jan 2022 19:54:47: 1000000 INFO @ Sat, 15 Jan 2022 19:54:51: 6000000 INFO @ Sat, 15 Jan 2022 19:54:52: 13000000 INFO @ Sat, 15 Jan 2022 19:54:54: 2000000 INFO @ Sat, 15 Jan 2022 19:54:57: 7000000 INFO @ Sat, 15 Jan 2022 19:54:58: 14000000 INFO @ Sat, 15 Jan 2022 19:55:01: 3000000 INFO @ Sat, 15 Jan 2022 19:55:04: 8000000 INFO @ Sat, 15 Jan 2022 19:55:04: 15000000 INFO @ Sat, 15 Jan 2022 19:55:08: 4000000 INFO @ Sat, 15 Jan 2022 19:55:11: 16000000 INFO @ Sat, 15 Jan 2022 19:55:11: 9000000 INFO @ Sat, 15 Jan 2022 19:55:14: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:55:14: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:55:14: #1 total tags in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:55:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:55:15: #1 tags after filtering in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:55:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:55:15: #1 finished! INFO @ Sat, 15 Jan 2022 19:55:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:55:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:55:15: 5000000 INFO @ Sat, 15 Jan 2022 19:55:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:55:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:55:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:55:18: 10000000 INFO @ Sat, 15 Jan 2022 19:55:22: 6000000 INFO @ Sat, 15 Jan 2022 19:55:24: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:55:29: 7000000 INFO @ Sat, 15 Jan 2022 19:55:31: 12000000 INFO @ Sat, 15 Jan 2022 19:55:36: 8000000 INFO @ Sat, 15 Jan 2022 19:55:38: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:55:43: 9000000 INFO @ Sat, 15 Jan 2022 19:55:45: 14000000 INFO @ Sat, 15 Jan 2022 19:55:50: 10000000 INFO @ Sat, 15 Jan 2022 19:55:51: 15000000 INFO @ Sat, 15 Jan 2022 19:55:57: 11000000 INFO @ Sat, 15 Jan 2022 19:55:58: 16000000 INFO @ Sat, 15 Jan 2022 19:56:02: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:56:02: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:56:02: #1 total tags in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:56:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:03: #1 tags after filtering in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:56:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:56:03: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:56:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:56:04: 12000000 INFO @ Sat, 15 Jan 2022 19:56:11: 13000000 INFO @ Sat, 15 Jan 2022 19:56:17: 14000000 INFO @ Sat, 15 Jan 2022 19:56:24: 15000000 INFO @ Sat, 15 Jan 2022 19:56:30: 16000000 INFO @ Sat, 15 Jan 2022 19:56:34: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:56:34: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:56:34: #1 total tags in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:56:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:56:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:56:34: #1 tags after filtering in treatment: 16625633 INFO @ Sat, 15 Jan 2022 19:56:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:56:34: #1 finished! INFO @ Sat, 15 Jan 2022 19:56:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:56:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:56:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:56:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:56:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065700/SRX8065700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling