Job ID = 14520993 SRX = SRX8065699 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 36201887 spots for SRR11489722/SRR11489722.sra Written 36201887 spots for SRR11489722/SRR11489722.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 36201887 reads; of these: 36201887 (100.00%) were unpaired; of these: 1000578 (2.76%) aligned 0 times 29975412 (82.80%) aligned exactly 1 time 5225897 (14.44%) aligned >1 times 97.24% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18735881 / 35201309 = 0.5322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:36: 1000000 INFO @ Sat, 15 Jan 2022 20:28:42: 2000000 INFO @ Sat, 15 Jan 2022 20:28:48: 3000000 INFO @ Sat, 15 Jan 2022 20:28:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:01: 5000000 INFO @ Sat, 15 Jan 2022 20:29:06: 1000000 INFO @ Sat, 15 Jan 2022 20:29:08: 6000000 INFO @ Sat, 15 Jan 2022 20:29:12: 2000000 INFO @ Sat, 15 Jan 2022 20:29:15: 7000000 INFO @ Sat, 15 Jan 2022 20:29:19: 3000000 INFO @ Sat, 15 Jan 2022 20:29:22: 8000000 INFO @ Sat, 15 Jan 2022 20:29:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:29:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:29:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:29:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:29:29: 9000000 INFO @ Sat, 15 Jan 2022 20:29:32: 5000000 INFO @ Sat, 15 Jan 2022 20:29:36: 1000000 INFO @ Sat, 15 Jan 2022 20:29:36: 10000000 INFO @ Sat, 15 Jan 2022 20:29:39: 6000000 INFO @ Sat, 15 Jan 2022 20:29:43: 11000000 INFO @ Sat, 15 Jan 2022 20:29:44: 2000000 INFO @ Sat, 15 Jan 2022 20:29:45: 7000000 INFO @ Sat, 15 Jan 2022 20:29:51: 12000000 INFO @ Sat, 15 Jan 2022 20:29:51: 3000000 INFO @ Sat, 15 Jan 2022 20:29:52: 8000000 INFO @ Sat, 15 Jan 2022 20:29:58: 13000000 INFO @ Sat, 15 Jan 2022 20:29:58: 4000000 INFO @ Sat, 15 Jan 2022 20:29:59: 9000000 INFO @ Sat, 15 Jan 2022 20:30:05: 14000000 INFO @ Sat, 15 Jan 2022 20:30:06: 10000000 INFO @ Sat, 15 Jan 2022 20:30:06: 5000000 INFO @ Sat, 15 Jan 2022 20:30:12: 11000000 INFO @ Sat, 15 Jan 2022 20:30:13: 15000000 INFO @ Sat, 15 Jan 2022 20:30:13: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:30:19: 12000000 INFO @ Sat, 15 Jan 2022 20:30:20: 16000000 INFO @ Sat, 15 Jan 2022 20:30:21: 7000000 INFO @ Sat, 15 Jan 2022 20:30:24: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:30:24: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:30:24: #1 total tags in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:30:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:24: #1 tags after filtering in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:30:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:30:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:24: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:30:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:26: 13000000 INFO @ Sat, 15 Jan 2022 20:30:28: 8000000 INFO @ Sat, 15 Jan 2022 20:30:32: 14000000 INFO @ Sat, 15 Jan 2022 20:30:35: 9000000 INFO @ Sat, 15 Jan 2022 20:30:39: 15000000 INFO @ Sat, 15 Jan 2022 20:30:43: 10000000 INFO @ Sat, 15 Jan 2022 20:30:46: 16000000 INFO @ Sat, 15 Jan 2022 20:30:49: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:30:49: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:30:49: #1 total tags in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:30:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:49: #1 tags after filtering in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:30:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:30:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:51: 11000000 INFO @ Sat, 15 Jan 2022 20:30:58: 12000000 INFO @ Sat, 15 Jan 2022 20:31:05: 13000000 INFO @ Sat, 15 Jan 2022 20:31:12: 14000000 INFO @ Sat, 15 Jan 2022 20:31:18: 15000000 INFO @ Sat, 15 Jan 2022 20:31:25: 16000000 INFO @ Sat, 15 Jan 2022 20:31:28: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 20:31:28: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 20:31:28: #1 total tags in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:31:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:31:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:31:29: #1 tags after filtering in treatment: 16465428 INFO @ Sat, 15 Jan 2022 20:31:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:31:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:31:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:31:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:31:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:31:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:31:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065699/SRX8065699.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling