Job ID = 14520992 SRX = SRX8065698 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25040957 spots for SRR11489721/SRR11489721.sra Written 25040957 spots for SRR11489721/SRR11489721.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:16 25040957 reads; of these: 25040957 (100.00%) were unpaired; of these: 807426 (3.22%) aligned 0 times 19466838 (77.74%) aligned exactly 1 time 4766693 (19.04%) aligned >1 times 96.78% overall alignment rate Time searching: 00:03:16 Overall time: 00:03:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11498580 / 24233531 = 0.4745 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:02: 1000000 INFO @ Sat, 15 Jan 2022 20:24:07: 2000000 INFO @ Sat, 15 Jan 2022 20:24:12: 3000000 INFO @ Sat, 15 Jan 2022 20:24:17: 4000000 INFO @ Sat, 15 Jan 2022 20:24:22: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:27: 6000000 INFO @ Sat, 15 Jan 2022 20:24:31: 1000000 INFO @ Sat, 15 Jan 2022 20:24:33: 7000000 INFO @ Sat, 15 Jan 2022 20:24:36: 2000000 INFO @ Sat, 15 Jan 2022 20:24:38: 8000000 INFO @ Sat, 15 Jan 2022 20:24:42: 3000000 INFO @ Sat, 15 Jan 2022 20:24:43: 9000000 INFO @ Sat, 15 Jan 2022 20:24:47: 4000000 INFO @ Sat, 15 Jan 2022 20:24:48: 10000000 INFO @ Sat, 15 Jan 2022 20:24:52: 5000000 INFO @ Sat, 15 Jan 2022 20:24:54: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:24:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:24:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:24:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:24:58: 6000000 INFO @ Sat, 15 Jan 2022 20:24:59: 12000000 INFO @ Sat, 15 Jan 2022 20:25:02: 1000000 INFO @ Sat, 15 Jan 2022 20:25:03: 7000000 INFO @ Sat, 15 Jan 2022 20:25:03: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:25:03: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:25:03: #1 total tags in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:25:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:03: #1 tags after filtering in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:25:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:03: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:08: 2000000 INFO @ Sat, 15 Jan 2022 20:25:09: 8000000 INFO @ Sat, 15 Jan 2022 20:25:14: 9000000 INFO @ Sat, 15 Jan 2022 20:25:15: 3000000 INFO @ Sat, 15 Jan 2022 20:25:20: 10000000 INFO @ Sat, 15 Jan 2022 20:25:21: 4000000 INFO @ Sat, 15 Jan 2022 20:25:25: 11000000 INFO @ Sat, 15 Jan 2022 20:25:27: 5000000 INFO @ Sat, 15 Jan 2022 20:25:30: 12000000 INFO @ Sat, 15 Jan 2022 20:25:33: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:25:35: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:25:35: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:25:35: #1 total tags in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:25:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:35: #1 tags after filtering in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:25:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:25:35: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:39: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:25:45: 8000000 INFO @ Sat, 15 Jan 2022 20:25:51: 9000000 INFO @ Sat, 15 Jan 2022 20:25:57: 10000000 INFO @ Sat, 15 Jan 2022 20:26:03: 11000000 INFO @ Sat, 15 Jan 2022 20:26:09: 12000000 INFO @ Sat, 15 Jan 2022 20:26:13: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 20:26:13: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 20:26:13: #1 total tags in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:26:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:13: #1 tags after filtering in treatment: 12734951 INFO @ Sat, 15 Jan 2022 20:26:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:26:13: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8065698/SRX8065698.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling