Job ID = 7110372 SRX = SRX8053692 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19175432 spots for SRR11477533/SRR11477533.sra Written 19175432 spots for SRR11477533/SRR11477533.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:19 19175432 reads; of these: 19175432 (100.00%) were paired; of these: 2782531 (14.51%) aligned concordantly 0 times 13886254 (72.42%) aligned concordantly exactly 1 time 2506647 (13.07%) aligned concordantly >1 times ---- 2782531 pairs aligned concordantly 0 times; of these: 956108 (34.36%) aligned discordantly 1 time ---- 1826423 pairs aligned 0 times concordantly or discordantly; of these: 3652846 mates make up the pairs; of these: 2689506 (73.63%) aligned 0 times 540711 (14.80%) aligned exactly 1 time 422629 (11.57%) aligned >1 times 92.99% overall alignment rate Time searching: 00:10:19 Overall time: 00:10:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3971958 / 17261155 = 0.2301 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:17:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:17:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:17:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:17:18: 1000000 INFO @ Wed, 22 Jul 2020 14:17:23: 2000000 INFO @ Wed, 22 Jul 2020 14:17:27: 3000000 INFO @ Wed, 22 Jul 2020 14:17:31: 4000000 INFO @ Wed, 22 Jul 2020 14:17:35: 5000000 INFO @ Wed, 22 Jul 2020 14:17:39: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:17:44: 7000000 INFO @ Wed, 22 Jul 2020 14:17:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:17:44: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:17:44: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:17:48: 8000000 INFO @ Wed, 22 Jul 2020 14:17:48: 1000000 INFO @ Wed, 22 Jul 2020 14:17:52: 9000000 INFO @ Wed, 22 Jul 2020 14:17:53: 2000000 INFO @ Wed, 22 Jul 2020 14:17:57: 10000000 INFO @ Wed, 22 Jul 2020 14:17:57: 3000000 INFO @ Wed, 22 Jul 2020 14:18:01: 11000000 INFO @ Wed, 22 Jul 2020 14:18:02: 4000000 INFO @ Wed, 22 Jul 2020 14:18:06: 12000000 INFO @ Wed, 22 Jul 2020 14:18:06: 5000000 INFO @ Wed, 22 Jul 2020 14:18:10: 13000000 INFO @ Wed, 22 Jul 2020 14:18:11: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:18:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:18:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:18:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:18:14: 14000000 INFO @ Wed, 22 Jul 2020 14:18:15: 7000000 INFO @ Wed, 22 Jul 2020 14:18:18: 1000000 INFO @ Wed, 22 Jul 2020 14:18:19: 15000000 INFO @ Wed, 22 Jul 2020 14:18:20: 8000000 INFO @ Wed, 22 Jul 2020 14:18:23: 2000000 INFO @ Wed, 22 Jul 2020 14:18:23: 16000000 INFO @ Wed, 22 Jul 2020 14:18:25: 9000000 INFO @ Wed, 22 Jul 2020 14:18:27: 3000000 INFO @ Wed, 22 Jul 2020 14:18:28: 17000000 INFO @ Wed, 22 Jul 2020 14:18:29: 10000000 INFO @ Wed, 22 Jul 2020 14:18:32: 4000000 INFO @ Wed, 22 Jul 2020 14:18:33: 18000000 INFO @ Wed, 22 Jul 2020 14:18:34: 11000000 INFO @ Wed, 22 Jul 2020 14:18:36: 5000000 INFO @ Wed, 22 Jul 2020 14:18:37: 19000000 INFO @ Wed, 22 Jul 2020 14:18:38: 12000000 INFO @ Wed, 22 Jul 2020 14:18:41: 6000000 INFO @ Wed, 22 Jul 2020 14:18:42: 20000000 INFO @ Wed, 22 Jul 2020 14:18:43: 13000000 INFO @ Wed, 22 Jul 2020 14:18:45: 7000000 INFO @ Wed, 22 Jul 2020 14:18:46: 21000000 INFO @ Wed, 22 Jul 2020 14:18:47: 14000000 INFO @ Wed, 22 Jul 2020 14:18:50: 8000000 INFO @ Wed, 22 Jul 2020 14:18:51: 22000000 INFO @ Wed, 22 Jul 2020 14:18:52: 15000000 INFO @ Wed, 22 Jul 2020 14:18:54: 9000000 INFO @ Wed, 22 Jul 2020 14:18:55: 23000000 INFO @ Wed, 22 Jul 2020 14:18:56: 16000000 INFO @ Wed, 22 Jul 2020 14:18:59: 10000000 INFO @ Wed, 22 Jul 2020 14:18:59: 24000000 INFO @ Wed, 22 Jul 2020 14:19:01: 17000000 INFO @ Wed, 22 Jul 2020 14:19:03: 11000000 INFO @ Wed, 22 Jul 2020 14:19:04: 25000000 INFO @ Wed, 22 Jul 2020 14:19:05: 18000000 INFO @ Wed, 22 Jul 2020 14:19:07: 12000000 INFO @ Wed, 22 Jul 2020 14:19:08: 26000000 INFO @ Wed, 22 Jul 2020 14:19:10: 19000000 INFO @ Wed, 22 Jul 2020 14:19:12: 13000000 INFO @ Wed, 22 Jul 2020 14:19:12: 27000000 INFO @ Wed, 22 Jul 2020 14:19:14: 20000000 INFO @ Wed, 22 Jul 2020 14:19:16: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:19:16: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:19:16: #1 total tags in treatment: 12584336 INFO @ Wed, 22 Jul 2020 14:19:16: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:19:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:19:16: #1 tags after filtering in treatment: 8812363 INFO @ Wed, 22 Jul 2020 14:19:16: #1 Redundant rate of treatment: 0.30 INFO @ Wed, 22 Jul 2020 14:19:16: #1 finished! INFO @ Wed, 22 Jul 2020 14:19:16: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:19:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:19:16: 14000000 INFO @ Wed, 22 Jul 2020 14:19:16: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:19:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:19:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:19:19: 21000000 INFO @ Wed, 22 Jul 2020 14:19:21: 15000000 INFO @ Wed, 22 Jul 2020 14:19:23: 22000000 INFO @ Wed, 22 Jul 2020 14:19:25: 16000000 INFO @ Wed, 22 Jul 2020 14:19:27: 23000000 INFO @ Wed, 22 Jul 2020 14:19:30: 17000000 INFO @ Wed, 22 Jul 2020 14:19:32: 24000000 INFO @ Wed, 22 Jul 2020 14:19:34: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:19:36: 25000000 INFO @ Wed, 22 Jul 2020 14:19:38: 19000000 INFO @ Wed, 22 Jul 2020 14:19:40: 26000000 INFO @ Wed, 22 Jul 2020 14:19:43: 20000000 INFO @ Wed, 22 Jul 2020 14:19:44: 27000000 INFO @ Wed, 22 Jul 2020 14:19:47: 21000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:19:48: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:19:48: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:19:48: #1 total tags in treatment: 12584336 INFO @ Wed, 22 Jul 2020 14:19:48: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:19:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:19:48: #1 tags after filtering in treatment: 8812363 INFO @ Wed, 22 Jul 2020 14:19:48: #1 Redundant rate of treatment: 0.30 INFO @ Wed, 22 Jul 2020 14:19:48: #1 finished! INFO @ Wed, 22 Jul 2020 14:19:48: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:19:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:19:48: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:19:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:19:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:19:51: 22000000 INFO @ Wed, 22 Jul 2020 14:19:56: 23000000 INFO @ Wed, 22 Jul 2020 14:20:00: 24000000 INFO @ Wed, 22 Jul 2020 14:20:04: 25000000 INFO @ Wed, 22 Jul 2020 14:20:08: 26000000 INFO @ Wed, 22 Jul 2020 14:20:12: 27000000 INFO @ Wed, 22 Jul 2020 14:20:16: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:20:16: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:20:16: #1 total tags in treatment: 12584336 INFO @ Wed, 22 Jul 2020 14:20:16: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:20:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:20:16: #1 tags after filtering in treatment: 8812363 INFO @ Wed, 22 Jul 2020 14:20:16: #1 Redundant rate of treatment: 0.30 INFO @ Wed, 22 Jul 2020 14:20:16: #1 finished! INFO @ Wed, 22 Jul 2020 14:20:16: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:20:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:20:16: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:20:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:20:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053692/SRX8053692.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling