Job ID = 7110357 SRX = SRX8053690 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 34376684 spots for SRR11477531/SRR11477531.sra Written 34376684 spots for SRR11477531/SRR11477531.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:45 34376684 reads; of these: 34376684 (100.00%) were paired; of these: 3841078 (11.17%) aligned concordantly 0 times 25206051 (73.32%) aligned concordantly exactly 1 time 5329555 (15.50%) aligned concordantly >1 times ---- 3841078 pairs aligned concordantly 0 times; of these: 1742921 (45.38%) aligned discordantly 1 time ---- 2098157 pairs aligned 0 times concordantly or discordantly; of these: 4196314 mates make up the pairs; of these: 2618752 (62.41%) aligned 0 times 685800 (16.34%) aligned exactly 1 time 891762 (21.25%) aligned >1 times 96.19% overall alignment rate Time searching: 00:18:45 Overall time: 00:18:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 8639770 / 32115240 = 0.2690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:31:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:31:16: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:31:16: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:31:22: 1000000 INFO @ Wed, 22 Jul 2020 14:31:27: 2000000 INFO @ Wed, 22 Jul 2020 14:31:32: 3000000 INFO @ Wed, 22 Jul 2020 14:31:37: 4000000 INFO @ Wed, 22 Jul 2020 14:31:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:31:46: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:31:46: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:31:47: 6000000 INFO @ Wed, 22 Jul 2020 14:31:52: 1000000 INFO @ Wed, 22 Jul 2020 14:31:53: 7000000 INFO @ Wed, 22 Jul 2020 14:31:57: 2000000 INFO @ Wed, 22 Jul 2020 14:31:58: 8000000 INFO @ Wed, 22 Jul 2020 14:32:03: 3000000 INFO @ Wed, 22 Jul 2020 14:32:03: 9000000 INFO @ Wed, 22 Jul 2020 14:32:08: 4000000 INFO @ Wed, 22 Jul 2020 14:32:09: 10000000 INFO @ Wed, 22 Jul 2020 14:32:14: 5000000 INFO @ Wed, 22 Jul 2020 14:32:14: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:32:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:32:16: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:32:16: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:32:19: 6000000 INFO @ Wed, 22 Jul 2020 14:32:20: 12000000 INFO @ Wed, 22 Jul 2020 14:32:23: 1000000 INFO @ Wed, 22 Jul 2020 14:32:25: 7000000 INFO @ Wed, 22 Jul 2020 14:32:26: 13000000 INFO @ Wed, 22 Jul 2020 14:32:29: 2000000 INFO @ Wed, 22 Jul 2020 14:32:32: 8000000 INFO @ Wed, 22 Jul 2020 14:32:32: 14000000 INFO @ Wed, 22 Jul 2020 14:32:36: 3000000 INFO @ Wed, 22 Jul 2020 14:32:38: 9000000 INFO @ Wed, 22 Jul 2020 14:32:38: 15000000 INFO @ Wed, 22 Jul 2020 14:32:42: 4000000 INFO @ Wed, 22 Jul 2020 14:32:44: 10000000 INFO @ Wed, 22 Jul 2020 14:32:44: 16000000 INFO @ Wed, 22 Jul 2020 14:32:49: 5000000 INFO @ Wed, 22 Jul 2020 14:32:50: 11000000 INFO @ Wed, 22 Jul 2020 14:32:50: 17000000 INFO @ Wed, 22 Jul 2020 14:32:55: 6000000 INFO @ Wed, 22 Jul 2020 14:32:56: 12000000 INFO @ Wed, 22 Jul 2020 14:32:56: 18000000 INFO @ Wed, 22 Jul 2020 14:33:02: 13000000 INFO @ Wed, 22 Jul 2020 14:33:02: 7000000 INFO @ Wed, 22 Jul 2020 14:33:02: 19000000 INFO @ Wed, 22 Jul 2020 14:33:08: 14000000 INFO @ Wed, 22 Jul 2020 14:33:08: 20000000 INFO @ Wed, 22 Jul 2020 14:33:08: 8000000 INFO @ Wed, 22 Jul 2020 14:33:14: 15000000 INFO @ Wed, 22 Jul 2020 14:33:14: 21000000 INFO @ Wed, 22 Jul 2020 14:33:15: 9000000 INFO @ Wed, 22 Jul 2020 14:33:20: 16000000 INFO @ Wed, 22 Jul 2020 14:33:20: 22000000 INFO @ Wed, 22 Jul 2020 14:33:21: 10000000 INFO @ Wed, 22 Jul 2020 14:33:26: 17000000 INFO @ Wed, 22 Jul 2020 14:33:26: 23000000 INFO @ Wed, 22 Jul 2020 14:33:28: 11000000 INFO @ Wed, 22 Jul 2020 14:33:32: 18000000 INFO @ Wed, 22 Jul 2020 14:33:32: 24000000 INFO @ Wed, 22 Jul 2020 14:33:34: 12000000 INFO @ Wed, 22 Jul 2020 14:33:38: 19000000 INFO @ Wed, 22 Jul 2020 14:33:38: 25000000 INFO @ Wed, 22 Jul 2020 14:33:41: 13000000 INFO @ Wed, 22 Jul 2020 14:33:44: 20000000 INFO @ Wed, 22 Jul 2020 14:33:44: 26000000 INFO @ Wed, 22 Jul 2020 14:33:47: 14000000 INFO @ Wed, 22 Jul 2020 14:33:49: 21000000 INFO @ Wed, 22 Jul 2020 14:33:50: 27000000 INFO @ Wed, 22 Jul 2020 14:33:54: 15000000 INFO @ Wed, 22 Jul 2020 14:33:55: 28000000 INFO @ Wed, 22 Jul 2020 14:33:55: 22000000 INFO @ Wed, 22 Jul 2020 14:34:00: 16000000 INFO @ Wed, 22 Jul 2020 14:34:01: 29000000 INFO @ Wed, 22 Jul 2020 14:34:01: 23000000 INFO @ Wed, 22 Jul 2020 14:34:07: 30000000 INFO @ Wed, 22 Jul 2020 14:34:07: 17000000 INFO @ Wed, 22 Jul 2020 14:34:07: 24000000 INFO @ Wed, 22 Jul 2020 14:34:12: 31000000 INFO @ Wed, 22 Jul 2020 14:34:13: 25000000 INFO @ Wed, 22 Jul 2020 14:34:14: 18000000 INFO @ Wed, 22 Jul 2020 14:34:18: 32000000 INFO @ Wed, 22 Jul 2020 14:34:19: 26000000 INFO @ Wed, 22 Jul 2020 14:34:20: 19000000 INFO @ Wed, 22 Jul 2020 14:34:24: 33000000 INFO @ Wed, 22 Jul 2020 14:34:25: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:34:27: 20000000 INFO @ Wed, 22 Jul 2020 14:34:30: 34000000 INFO @ Wed, 22 Jul 2020 14:34:30: 28000000 INFO @ Wed, 22 Jul 2020 14:34:34: 21000000 INFO @ Wed, 22 Jul 2020 14:34:36: 35000000 INFO @ Wed, 22 Jul 2020 14:34:36: 29000000 INFO @ Wed, 22 Jul 2020 14:34:40: 22000000 INFO @ Wed, 22 Jul 2020 14:34:41: 36000000 INFO @ Wed, 22 Jul 2020 14:34:42: 30000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:34:47: 23000000 INFO @ Wed, 22 Jul 2020 14:34:47: 37000000 INFO @ Wed, 22 Jul 2020 14:34:48: 31000000 INFO @ Wed, 22 Jul 2020 14:34:53: 38000000 INFO @ Wed, 22 Jul 2020 14:34:54: 24000000 INFO @ Wed, 22 Jul 2020 14:34:54: 32000000 INFO @ Wed, 22 Jul 2020 14:34:59: 39000000 INFO @ Wed, 22 Jul 2020 14:35:00: 33000000 INFO @ Wed, 22 Jul 2020 14:35:00: 25000000 INFO @ Wed, 22 Jul 2020 14:35:05: 40000000 INFO @ Wed, 22 Jul 2020 14:35:06: 34000000 INFO @ Wed, 22 Jul 2020 14:35:07: 26000000 INFO @ Wed, 22 Jul 2020 14:35:11: 41000000 INFO @ Wed, 22 Jul 2020 14:35:12: 35000000 INFO @ Wed, 22 Jul 2020 14:35:13: 27000000 INFO @ Wed, 22 Jul 2020 14:35:17: 42000000 INFO @ Wed, 22 Jul 2020 14:35:18: 36000000 INFO @ Wed, 22 Jul 2020 14:35:20: 28000000 INFO @ Wed, 22 Jul 2020 14:35:23: 43000000 INFO @ Wed, 22 Jul 2020 14:35:24: 37000000 INFO @ Wed, 22 Jul 2020 14:35:26: 29000000 INFO @ Wed, 22 Jul 2020 14:35:28: 44000000 INFO @ Wed, 22 Jul 2020 14:35:29: 38000000 INFO @ Wed, 22 Jul 2020 14:35:33: 30000000 INFO @ Wed, 22 Jul 2020 14:35:34: 45000000 INFO @ Wed, 22 Jul 2020 14:35:35: 39000000 INFO @ Wed, 22 Jul 2020 14:35:40: 31000000 INFO @ Wed, 22 Jul 2020 14:35:40: 46000000 INFO @ Wed, 22 Jul 2020 14:35:41: 40000000 INFO @ Wed, 22 Jul 2020 14:35:46: 47000000 INFO @ Wed, 22 Jul 2020 14:35:46: 32000000 INFO @ Wed, 22 Jul 2020 14:35:47: 41000000 INFO @ Wed, 22 Jul 2020 14:35:52: 48000000 INFO @ Wed, 22 Jul 2020 14:35:53: 33000000 INFO @ Wed, 22 Jul 2020 14:35:53: 42000000 INFO @ Wed, 22 Jul 2020 14:35:57: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:35:57: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:35:57: #1 total tags in treatment: 22327974 INFO @ Wed, 22 Jul 2020 14:35:57: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:35:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:35:57: #1 tags after filtering in treatment: 13174456 INFO @ Wed, 22 Jul 2020 14:35:57: #1 Redundant rate of treatment: 0.41 INFO @ Wed, 22 Jul 2020 14:35:57: #1 finished! INFO @ Wed, 22 Jul 2020 14:35:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:35:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:35:58: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:35:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:35:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:35:59: 43000000 INFO @ Wed, 22 Jul 2020 14:35:59: 34000000 INFO @ Wed, 22 Jul 2020 14:36:05: 44000000 INFO @ Wed, 22 Jul 2020 14:36:06: 35000000 INFO @ Wed, 22 Jul 2020 14:36:10: 45000000 INFO @ Wed, 22 Jul 2020 14:36:12: 36000000 INFO @ Wed, 22 Jul 2020 14:36:16: 46000000 INFO @ Wed, 22 Jul 2020 14:36:19: 37000000 INFO @ Wed, 22 Jul 2020 14:36:22: 47000000 INFO @ Wed, 22 Jul 2020 14:36:25: 38000000 INFO @ Wed, 22 Jul 2020 14:36:27: 48000000 INFO @ Wed, 22 Jul 2020 14:36:32: 39000000 INFO @ Wed, 22 Jul 2020 14:36:32: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:36:32: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:36:32: #1 total tags in treatment: 22327974 INFO @ Wed, 22 Jul 2020 14:36:32: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:36:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:36:33: #1 tags after filtering in treatment: 13174456 INFO @ Wed, 22 Jul 2020 14:36:33: #1 Redundant rate of treatment: 0.41 INFO @ Wed, 22 Jul 2020 14:36:33: #1 finished! INFO @ Wed, 22 Jul 2020 14:36:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:36:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:36:34: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:36:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:36:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:36:38: 40000000 INFO @ Wed, 22 Jul 2020 14:36:44: 41000000 INFO @ Wed, 22 Jul 2020 14:36:50: 42000000 INFO @ Wed, 22 Jul 2020 14:36:56: 43000000 INFO @ Wed, 22 Jul 2020 14:37:02: 44000000 INFO @ Wed, 22 Jul 2020 14:37:08: 45000000 INFO @ Wed, 22 Jul 2020 14:37:14: 46000000 INFO @ Wed, 22 Jul 2020 14:37:20: 47000000 INFO @ Wed, 22 Jul 2020 14:37:26: 48000000 INFO @ Wed, 22 Jul 2020 14:37:32: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 14:37:32: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 14:37:32: #1 total tags in treatment: 22327974 INFO @ Wed, 22 Jul 2020 14:37:32: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:37:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:37:32: #1 tags after filtering in treatment: 13174456 INFO @ Wed, 22 Jul 2020 14:37:32: #1 Redundant rate of treatment: 0.41 INFO @ Wed, 22 Jul 2020 14:37:32: #1 finished! INFO @ Wed, 22 Jul 2020 14:37:32: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:37:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:37:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:37:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:37:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8053690/SRX8053690.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling