Job ID = 7109698 SRX = SRX8036510 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14459213 spots for SRR11458957/SRR11458957.sra Written 14459213 spots for SRR11458957/SRR11458957.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 14459213 reads; of these: 14459213 (100.00%) were paired; of these: 13387114 (92.59%) aligned concordantly 0 times 843698 (5.84%) aligned concordantly exactly 1 time 228401 (1.58%) aligned concordantly >1 times ---- 13387114 pairs aligned concordantly 0 times; of these: 2738 (0.02%) aligned discordantly 1 time ---- 13384376 pairs aligned 0 times concordantly or discordantly; of these: 26768752 mates make up the pairs; of these: 26759359 (99.96%) aligned 0 times 3885 (0.01%) aligned exactly 1 time 5508 (0.02%) aligned >1 times 7.47% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 190244 / 1074674 = 0.1770 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:54:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:54:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:54:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:54:30: 1000000 INFO @ Wed, 22 Jul 2020 13:54:37: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:54:37: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:54:37: #1 total tags in treatment: 882111 INFO @ Wed, 22 Jul 2020 13:54:37: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:54:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:54:37: #1 tags after filtering in treatment: 753097 INFO @ Wed, 22 Jul 2020 13:54:37: #1 Redundant rate of treatment: 0.15 INFO @ Wed, 22 Jul 2020 13:54:37: #1 finished! INFO @ Wed, 22 Jul 2020 13:54:37: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:54:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:54:37: #2 number of paired peaks: 35 WARNING @ Wed, 22 Jul 2020 13:54:37: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:54:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:54:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:54:52: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:54:52: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:54:59: 1000000 INFO @ Wed, 22 Jul 2020 13:55:05: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:55:05: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:55:05: #1 total tags in treatment: 882111 INFO @ Wed, 22 Jul 2020 13:55:05: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:55:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:55:05: #1 tags after filtering in treatment: 753097 INFO @ Wed, 22 Jul 2020 13:55:05: #1 Redundant rate of treatment: 0.15 INFO @ Wed, 22 Jul 2020 13:55:05: #1 finished! INFO @ Wed, 22 Jul 2020 13:55:05: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:55:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:55:05: #2 number of paired peaks: 35 WARNING @ Wed, 22 Jul 2020 13:55:05: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:55:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:55:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:55:22: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:55:22: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:55:29: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:55:36: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 13:55:36: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 13:55:36: #1 total tags in treatment: 882111 INFO @ Wed, 22 Jul 2020 13:55:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:55:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:55:36: #1 tags after filtering in treatment: 753097 INFO @ Wed, 22 Jul 2020 13:55:36: #1 Redundant rate of treatment: 0.15 INFO @ Wed, 22 Jul 2020 13:55:36: #1 finished! INFO @ Wed, 22 Jul 2020 13:55:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:55:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:55:36: #2 number of paired peaks: 35 WARNING @ Wed, 22 Jul 2020 13:55:36: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:55:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036510/SRX8036510.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling