Job ID = 7109562 SRX = SRX8036507 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15541754 spots for SRR11458954/SRR11458954.sra Written 15541754 spots for SRR11458954/SRR11458954.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:44 15541754 reads; of these: 15541754 (100.00%) were paired; of these: 10145248 (65.28%) aligned concordantly 0 times 4599753 (29.60%) aligned concordantly exactly 1 time 796753 (5.13%) aligned concordantly >1 times ---- 10145248 pairs aligned concordantly 0 times; of these: 77503 (0.76%) aligned discordantly 1 time ---- 10067745 pairs aligned 0 times concordantly or discordantly; of these: 20135490 mates make up the pairs; of these: 20058303 (99.62%) aligned 0 times 38701 (0.19%) aligned exactly 1 time 38486 (0.19%) aligned >1 times 35.47% overall alignment rate Time searching: 00:09:44 Overall time: 00:09:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 812441 / 5469339 = 0.1485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:01:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:01:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:01:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:01:23: 1000000 INFO @ Wed, 22 Jul 2020 14:01:32: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:01:42: 3000000 INFO @ Wed, 22 Jul 2020 14:01:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:01:44: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:01:44: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:01:52: 4000000 INFO @ Wed, 22 Jul 2020 14:01:54: 1000000 INFO @ Wed, 22 Jul 2020 14:02:03: 5000000 INFO @ Wed, 22 Jul 2020 14:02:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:02:13: 6000000 INFO @ Wed, 22 Jul 2020 14:02:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:02:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:02:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:02:16: 3000000 INFO @ Wed, 22 Jul 2020 14:02:23: 1000000 INFO @ Wed, 22 Jul 2020 14:02:24: 7000000 INFO @ Wed, 22 Jul 2020 14:02:26: 4000000 INFO @ Wed, 22 Jul 2020 14:02:32: 2000000 INFO @ Wed, 22 Jul 2020 14:02:35: 8000000 INFO @ Wed, 22 Jul 2020 14:02:37: 5000000 INFO @ Wed, 22 Jul 2020 14:02:40: 3000000 INFO @ Wed, 22 Jul 2020 14:02:45: 9000000 INFO @ Wed, 22 Jul 2020 14:02:48: 6000000 INFO @ Wed, 22 Jul 2020 14:02:49: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:02:49: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:02:49: #1 total tags in treatment: 4591909 INFO @ Wed, 22 Jul 2020 14:02:49: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:02:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:02:49: 4000000 INFO @ Wed, 22 Jul 2020 14:02:49: #1 tags after filtering in treatment: 3691124 INFO @ Wed, 22 Jul 2020 14:02:49: #1 Redundant rate of treatment: 0.20 INFO @ Wed, 22 Jul 2020 14:02:49: #1 finished! INFO @ Wed, 22 Jul 2020 14:02:49: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:02:50: #2 number of paired peaks: 42 WARNING @ Wed, 22 Jul 2020 14:02:50: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:02:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:02:58: 5000000 INFO @ Wed, 22 Jul 2020 14:02:58: 7000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:03:07: 6000000 INFO @ Wed, 22 Jul 2020 14:03:09: 8000000 INFO @ Wed, 22 Jul 2020 14:03:16: 7000000 INFO @ Wed, 22 Jul 2020 14:03:19: 9000000 INFO @ Wed, 22 Jul 2020 14:03:23: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:03:23: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:03:23: #1 total tags in treatment: 4591909 INFO @ Wed, 22 Jul 2020 14:03:23: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:03:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:03:23: #1 tags after filtering in treatment: 3691124 INFO @ Wed, 22 Jul 2020 14:03:23: #1 Redundant rate of treatment: 0.20 INFO @ Wed, 22 Jul 2020 14:03:23: #1 finished! INFO @ Wed, 22 Jul 2020 14:03:23: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:03:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:03:24: #2 number of paired peaks: 42 WARNING @ Wed, 22 Jul 2020 14:03:24: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:03:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:03:24: 8000000 INFO @ Wed, 22 Jul 2020 14:03:32: 9000000 INFO @ Wed, 22 Jul 2020 14:03:35: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:03:35: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:03:35: #1 total tags in treatment: 4591909 INFO @ Wed, 22 Jul 2020 14:03:35: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:03:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:03:35: #1 tags after filtering in treatment: 3691124 INFO @ Wed, 22 Jul 2020 14:03:35: #1 Redundant rate of treatment: 0.20 INFO @ Wed, 22 Jul 2020 14:03:35: #1 finished! INFO @ Wed, 22 Jul 2020 14:03:35: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:03:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:03:35: #2 number of paired peaks: 42 WARNING @ Wed, 22 Jul 2020 14:03:35: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:03:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036507/SRX8036507.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling