Job ID = 7109273 SRX = SRX8036502 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11633907 spots for SRR11458949/SRR11458949.sra Written 11633907 spots for SRR11458949/SRR11458949.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:34 11633907 reads; of these: 11633907 (100.00%) were paired; of these: 453566 (3.90%) aligned concordantly 0 times 9057654 (77.86%) aligned concordantly exactly 1 time 2122687 (18.25%) aligned concordantly >1 times ---- 453566 pairs aligned concordantly 0 times; of these: 64324 (14.18%) aligned discordantly 1 time ---- 389242 pairs aligned 0 times concordantly or discordantly; of these: 778484 mates make up the pairs; of these: 676603 (86.91%) aligned 0 times 56376 (7.24%) aligned exactly 1 time 45505 (5.85%) aligned >1 times 97.09% overall alignment rate Time searching: 00:14:34 Overall time: 00:14:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2077584 / 11236702 = 0.1849 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:07:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:07:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:07:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:07:23: 1000000 INFO @ Wed, 22 Jul 2020 14:07:33: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:07:42: 3000000 INFO @ Wed, 22 Jul 2020 14:07:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:07:44: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:07:44: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:07:54: 4000000 INFO @ Wed, 22 Jul 2020 14:07:55: 1000000 INFO @ Wed, 22 Jul 2020 14:08:05: 5000000 INFO @ Wed, 22 Jul 2020 14:08:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 14:08:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 14:08:14: #1 read tag files... INFO @ Wed, 22 Jul 2020 14:08:14: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 14:08:16: 3000000 INFO @ Wed, 22 Jul 2020 14:08:17: 6000000 INFO @ Wed, 22 Jul 2020 14:08:25: 1000000 INFO @ Wed, 22 Jul 2020 14:08:27: 4000000 INFO @ Wed, 22 Jul 2020 14:08:29: 7000000 INFO @ Wed, 22 Jul 2020 14:08:36: 2000000 INFO @ Wed, 22 Jul 2020 14:08:39: 5000000 INFO @ Wed, 22 Jul 2020 14:08:41: 8000000 INFO @ Wed, 22 Jul 2020 14:08:48: 3000000 INFO @ Wed, 22 Jul 2020 14:08:50: 6000000 INFO @ Wed, 22 Jul 2020 14:08:53: 9000000 INFO @ Wed, 22 Jul 2020 14:08:59: 4000000 INFO @ Wed, 22 Jul 2020 14:09:02: 7000000 INFO @ Wed, 22 Jul 2020 14:09:05: 10000000 INFO @ Wed, 22 Jul 2020 14:09:10: 5000000 INFO @ Wed, 22 Jul 2020 14:09:13: 8000000 INFO @ Wed, 22 Jul 2020 14:09:16: 11000000 INFO @ Wed, 22 Jul 2020 14:09:22: 6000000 INFO @ Wed, 22 Jul 2020 14:09:25: 9000000 INFO @ Wed, 22 Jul 2020 14:09:28: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:09:33: 7000000 INFO @ Wed, 22 Jul 2020 14:09:36: 10000000 INFO @ Wed, 22 Jul 2020 14:09:40: 13000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:09:45: 8000000 INFO @ Wed, 22 Jul 2020 14:09:48: 11000000 INFO @ Wed, 22 Jul 2020 14:09:52: 14000000 INFO @ Wed, 22 Jul 2020 14:09:56: 9000000 INFO @ Wed, 22 Jul 2020 14:09:59: 12000000 INFO @ Wed, 22 Jul 2020 14:10:03: 15000000 INFO @ Wed, 22 Jul 2020 14:10:07: 10000000 INFO @ Wed, 22 Jul 2020 14:10:11: 13000000 INFO @ Wed, 22 Jul 2020 14:10:14: 16000000 INFO @ Wed, 22 Jul 2020 14:10:18: 11000000 INFO @ Wed, 22 Jul 2020 14:10:22: 14000000 INFO @ Wed, 22 Jul 2020 14:10:25: 17000000 INFO @ Wed, 22 Jul 2020 14:10:30: 12000000 INFO @ Wed, 22 Jul 2020 14:10:33: 15000000 INFO @ Wed, 22 Jul 2020 14:10:36: 18000000 INFO @ Wed, 22 Jul 2020 14:10:41: 13000000 INFO @ Wed, 22 Jul 2020 14:10:41: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:10:41: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:10:41: #1 total tags in treatment: 9109085 INFO @ Wed, 22 Jul 2020 14:10:41: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:10:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:10:41: #1 tags after filtering in treatment: 6529574 INFO @ Wed, 22 Jul 2020 14:10:41: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 14:10:41: #1 finished! INFO @ Wed, 22 Jul 2020 14:10:41: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:10:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:10:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:10:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:10:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:10:44: 16000000 INFO @ Wed, 22 Jul 2020 14:10:51: 14000000 INFO @ Wed, 22 Jul 2020 14:10:55: 17000000 INFO @ Wed, 22 Jul 2020 14:11:02: 15000000 INFO @ Wed, 22 Jul 2020 14:11:05: 18000000 INFO @ Wed, 22 Jul 2020 14:11:10: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:11:10: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:11:10: #1 total tags in treatment: 9109085 INFO @ Wed, 22 Jul 2020 14:11:10: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:11:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:11:10: #1 tags after filtering in treatment: 6529574 INFO @ Wed, 22 Jul 2020 14:11:10: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 14:11:10: #1 finished! INFO @ Wed, 22 Jul 2020 14:11:10: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:11:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:11:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:11:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:11:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 14:11:12: 16000000 INFO @ Wed, 22 Jul 2020 14:11:22: 17000000 INFO @ Wed, 22 Jul 2020 14:11:32: 18000000 INFO @ Wed, 22 Jul 2020 14:11:36: #1 tag size is determined as 150 bps INFO @ Wed, 22 Jul 2020 14:11:36: #1 tag size = 150 INFO @ Wed, 22 Jul 2020 14:11:36: #1 total tags in treatment: 9109085 INFO @ Wed, 22 Jul 2020 14:11:36: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:11:36: #1 tags after filtering in treatment: 6529574 INFO @ Wed, 22 Jul 2020 14:11:36: #1 Redundant rate of treatment: 0.28 INFO @ Wed, 22 Jul 2020 14:11:36: #1 finished! INFO @ Wed, 22 Jul 2020 14:11:36: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:11:36: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:11:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:11:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8036502/SRX8036502.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling