Job ID = 14519676 SRX = SRX7973303 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32189806 spots for SRR11394486/SRR11394486.sra Written 32189806 spots for SRR11394486/SRR11394486.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:42 32189806 reads; of these: 32189806 (100.00%) were paired; of these: 11140912 (34.61%) aligned concordantly 0 times 16770576 (52.10%) aligned concordantly exactly 1 time 4278318 (13.29%) aligned concordantly >1 times ---- 11140912 pairs aligned concordantly 0 times; of these: 468210 (4.20%) aligned discordantly 1 time ---- 10672702 pairs aligned 0 times concordantly or discordantly; of these: 21345404 mates make up the pairs; of these: 20770272 (97.31%) aligned 0 times 237918 (1.11%) aligned exactly 1 time 337214 (1.58%) aligned >1 times 67.74% overall alignment rate Time searching: 00:10:42 Overall time: 00:10:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1661703 / 21513454 = 0.0772 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:41:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:41:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:41:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:41:40: 1000000 INFO @ Sat, 15 Jan 2022 17:41:47: 2000000 INFO @ Sat, 15 Jan 2022 17:41:53: 3000000 INFO @ Sat, 15 Jan 2022 17:41:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:06: 5000000 INFO @ Sat, 15 Jan 2022 17:42:11: 1000000 INFO @ Sat, 15 Jan 2022 17:42:13: 6000000 INFO @ Sat, 15 Jan 2022 17:42:17: 2000000 INFO @ Sat, 15 Jan 2022 17:42:20: 7000000 INFO @ Sat, 15 Jan 2022 17:42:23: 3000000 INFO @ Sat, 15 Jan 2022 17:42:27: 8000000 INFO @ Sat, 15 Jan 2022 17:42:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:34: 9000000 INFO @ Sat, 15 Jan 2022 17:42:36: 5000000 INFO @ Sat, 15 Jan 2022 17:42:41: 1000000 INFO @ Sat, 15 Jan 2022 17:42:41: 10000000 INFO @ Sat, 15 Jan 2022 17:42:42: 6000000 INFO @ Sat, 15 Jan 2022 17:42:48: 7000000 INFO @ Sat, 15 Jan 2022 17:42:48: 2000000 INFO @ Sat, 15 Jan 2022 17:42:49: 11000000 INFO @ Sat, 15 Jan 2022 17:42:55: 8000000 INFO @ Sat, 15 Jan 2022 17:42:56: 12000000 INFO @ Sat, 15 Jan 2022 17:42:56: 3000000 INFO @ Sat, 15 Jan 2022 17:43:01: 9000000 INFO @ Sat, 15 Jan 2022 17:43:03: 13000000 INFO @ Sat, 15 Jan 2022 17:43:03: 4000000 INFO @ Sat, 15 Jan 2022 17:43:07: 10000000 INFO @ Sat, 15 Jan 2022 17:43:10: 14000000 INFO @ Sat, 15 Jan 2022 17:43:11: 5000000 INFO @ Sat, 15 Jan 2022 17:43:14: 11000000 INFO @ Sat, 15 Jan 2022 17:43:18: 15000000 INFO @ Sat, 15 Jan 2022 17:43:18: 6000000 INFO @ Sat, 15 Jan 2022 17:43:20: 12000000 INFO @ Sat, 15 Jan 2022 17:43:25: 16000000 INFO @ Sat, 15 Jan 2022 17:43:25: 7000000 INFO @ Sat, 15 Jan 2022 17:43:26: 13000000 INFO @ Sat, 15 Jan 2022 17:43:32: 17000000 INFO @ Sat, 15 Jan 2022 17:43:32: 8000000 INFO @ Sat, 15 Jan 2022 17:43:33: 14000000 INFO @ Sat, 15 Jan 2022 17:43:39: 15000000 INFO @ Sat, 15 Jan 2022 17:43:39: 18000000 INFO @ Sat, 15 Jan 2022 17:43:40: 9000000 INFO @ Sat, 15 Jan 2022 17:43:45: 16000000 INFO @ Sat, 15 Jan 2022 17:43:46: 19000000 INFO @ Sat, 15 Jan 2022 17:43:47: 10000000 INFO @ Sat, 15 Jan 2022 17:43:51: 17000000 INFO @ Sat, 15 Jan 2022 17:43:54: 20000000 INFO @ Sat, 15 Jan 2022 17:43:54: 11000000 INFO @ Sat, 15 Jan 2022 17:43:58: 18000000 INFO @ Sat, 15 Jan 2022 17:44:01: 21000000 INFO @ Sat, 15 Jan 2022 17:44:01: 12000000 INFO @ Sat, 15 Jan 2022 17:44:04: 19000000 INFO @ Sat, 15 Jan 2022 17:44:08: 22000000 INFO @ Sat, 15 Jan 2022 17:44:08: 13000000 INFO @ Sat, 15 Jan 2022 17:44:10: 20000000 INFO @ Sat, 15 Jan 2022 17:44:15: 23000000 INFO @ Sat, 15 Jan 2022 17:44:16: 14000000 INFO @ Sat, 15 Jan 2022 17:44:17: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:44:22: 24000000 INFO @ Sat, 15 Jan 2022 17:44:23: 22000000 INFO @ Sat, 15 Jan 2022 17:44:23: 15000000 INFO @ Sat, 15 Jan 2022 17:44:29: 23000000 INFO @ Sat, 15 Jan 2022 17:44:29: 25000000 INFO @ Sat, 15 Jan 2022 17:44:30: 16000000 INFO @ Sat, 15 Jan 2022 17:44:36: 24000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:44:36: 26000000 INFO @ Sat, 15 Jan 2022 17:44:38: 17000000 INFO @ Sat, 15 Jan 2022 17:44:42: 25000000 INFO @ Sat, 15 Jan 2022 17:44:44: 27000000 INFO @ Sat, 15 Jan 2022 17:44:45: 18000000 INFO @ Sat, 15 Jan 2022 17:44:48: 26000000 INFO @ Sat, 15 Jan 2022 17:44:51: 28000000 INFO @ Sat, 15 Jan 2022 17:44:52: 19000000 INFO @ Sat, 15 Jan 2022 17:44:54: 27000000 INFO @ Sat, 15 Jan 2022 17:44:58: 29000000 INFO @ Sat, 15 Jan 2022 17:44:59: 20000000 INFO @ Sat, 15 Jan 2022 17:45:01: 28000000 INFO @ Sat, 15 Jan 2022 17:45:06: 30000000 INFO @ Sat, 15 Jan 2022 17:45:07: 21000000 INFO @ Sat, 15 Jan 2022 17:45:07: 29000000 INFO @ Sat, 15 Jan 2022 17:45:13: 31000000 INFO @ Sat, 15 Jan 2022 17:45:13: 30000000 INFO @ Sat, 15 Jan 2022 17:45:14: 22000000 INFO @ Sat, 15 Jan 2022 17:45:20: 31000000 INFO @ Sat, 15 Jan 2022 17:45:20: 32000000 INFO @ Sat, 15 Jan 2022 17:45:21: 23000000 INFO @ Sat, 15 Jan 2022 17:45:26: 32000000 INFO @ Sat, 15 Jan 2022 17:45:27: 33000000 INFO @ Sat, 15 Jan 2022 17:45:28: 24000000 INFO @ Sat, 15 Jan 2022 17:45:32: 33000000 INFO @ Sat, 15 Jan 2022 17:45:34: 34000000 INFO @ Sat, 15 Jan 2022 17:45:36: 25000000 INFO @ Sat, 15 Jan 2022 17:45:39: 34000000 INFO @ Sat, 15 Jan 2022 17:45:41: 35000000 INFO @ Sat, 15 Jan 2022 17:45:43: 26000000 INFO @ Sat, 15 Jan 2022 17:45:45: 35000000 INFO @ Sat, 15 Jan 2022 17:45:49: 36000000 INFO @ Sat, 15 Jan 2022 17:45:50: 27000000 INFO @ Sat, 15 Jan 2022 17:45:51: 36000000 INFO @ Sat, 15 Jan 2022 17:45:56: 37000000 INFO @ Sat, 15 Jan 2022 17:45:57: 37000000 INFO @ Sat, 15 Jan 2022 17:45:58: 28000000 INFO @ Sat, 15 Jan 2022 17:46:03: 38000000 INFO @ Sat, 15 Jan 2022 17:46:04: 38000000 INFO @ Sat, 15 Jan 2022 17:46:05: 29000000 INFO @ Sat, 15 Jan 2022 17:46:10: 39000000 INFO @ Sat, 15 Jan 2022 17:46:11: 39000000 INFO @ Sat, 15 Jan 2022 17:46:12: 30000000 INFO @ Sat, 15 Jan 2022 17:46:17: 40000000 INFO @ Sat, 15 Jan 2022 17:46:18: 40000000 INFO @ Sat, 15 Jan 2022 17:46:19: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:46:19: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:46:19: #1 total tags in treatment: 19401107 INFO @ Sat, 15 Jan 2022 17:46:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:19: 31000000 INFO @ Sat, 15 Jan 2022 17:46:20: #1 tags after filtering in treatment: 11613286 INFO @ Sat, 15 Jan 2022 17:46:20: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 17:46:20: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:20: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:46:20: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:46:20: #1 total tags in treatment: 19401107 INFO @ Sat, 15 Jan 2022 17:46:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:46:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:46:21: #1 tags after filtering in treatment: 11613286 INFO @ Sat, 15 Jan 2022 17:46:21: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 17:46:21: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:46:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:46:26: 32000000 INFO @ Sat, 15 Jan 2022 17:46:32: 33000000 INFO @ Sat, 15 Jan 2022 17:46:39: 34000000 INFO @ Sat, 15 Jan 2022 17:46:45: 35000000 INFO @ Sat, 15 Jan 2022 17:46:51: 36000000 INFO @ Sat, 15 Jan 2022 17:46:57: 37000000 INFO @ Sat, 15 Jan 2022 17:47:04: 38000000 INFO @ Sat, 15 Jan 2022 17:47:10: 39000000 INFO @ Sat, 15 Jan 2022 17:47:16: 40000000 INFO @ Sat, 15 Jan 2022 17:47:18: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:47:18: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:47:18: #1 total tags in treatment: 19401107 INFO @ Sat, 15 Jan 2022 17:47:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:47:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:47:18: #1 tags after filtering in treatment: 11613286 INFO @ Sat, 15 Jan 2022 17:47:18: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 17:47:18: #1 finished! INFO @ Sat, 15 Jan 2022 17:47:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:47:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:47:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:47:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:47:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973303/SRX7973303.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling