Job ID = 14519675 SRX = SRX7973302 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32606732 spots for SRR11394485/SRR11394485.sra Written 32606732 spots for SRR11394485/SRR11394485.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:38 32606732 reads; of these: 32606732 (100.00%) were paired; of these: 4631914 (14.21%) aligned concordantly 0 times 25344232 (77.73%) aligned concordantly exactly 1 time 2630586 (8.07%) aligned concordantly >1 times ---- 4631914 pairs aligned concordantly 0 times; of these: 1032039 (22.28%) aligned discordantly 1 time ---- 3599875 pairs aligned 0 times concordantly or discordantly; of these: 7199750 mates make up the pairs; of these: 6664369 (92.56%) aligned 0 times 274108 (3.81%) aligned exactly 1 time 261273 (3.63%) aligned >1 times 89.78% overall alignment rate Time searching: 00:14:38 Overall time: 00:14:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1047121 / 28994870 = 0.0361 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:50:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:50:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:50:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:50:10: 1000000 INFO @ Sat, 15 Jan 2022 17:50:16: 2000000 INFO @ Sat, 15 Jan 2022 17:50:21: 3000000 INFO @ Sat, 15 Jan 2022 17:50:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:50:34: 5000000 INFO @ Sat, 15 Jan 2022 17:50:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:50:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:50:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:50:40: 6000000 INFO @ Sat, 15 Jan 2022 17:50:40: 1000000 INFO @ Sat, 15 Jan 2022 17:50:46: 2000000 INFO @ Sat, 15 Jan 2022 17:50:46: 7000000 INFO @ Sat, 15 Jan 2022 17:50:52: 3000000 INFO @ Sat, 15 Jan 2022 17:50:52: 8000000 INFO @ Sat, 15 Jan 2022 17:50:58: 4000000 INFO @ Sat, 15 Jan 2022 17:50:58: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:51:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:51:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:51:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:51:04: 5000000 INFO @ Sat, 15 Jan 2022 17:51:04: 10000000 INFO @ Sat, 15 Jan 2022 17:51:10: 1000000 INFO @ Sat, 15 Jan 2022 17:51:10: 6000000 INFO @ Sat, 15 Jan 2022 17:51:11: 11000000 INFO @ Sat, 15 Jan 2022 17:51:16: 2000000 INFO @ Sat, 15 Jan 2022 17:51:16: 7000000 INFO @ Sat, 15 Jan 2022 17:51:17: 12000000 INFO @ Sat, 15 Jan 2022 17:51:22: 3000000 INFO @ Sat, 15 Jan 2022 17:51:23: 8000000 INFO @ Sat, 15 Jan 2022 17:51:23: 13000000 INFO @ Sat, 15 Jan 2022 17:51:28: 4000000 INFO @ Sat, 15 Jan 2022 17:51:29: 9000000 INFO @ Sat, 15 Jan 2022 17:51:29: 14000000 INFO @ Sat, 15 Jan 2022 17:51:34: 5000000 INFO @ Sat, 15 Jan 2022 17:51:34: 15000000 INFO @ Sat, 15 Jan 2022 17:51:35: 10000000 INFO @ Sat, 15 Jan 2022 17:51:40: 6000000 INFO @ Sat, 15 Jan 2022 17:51:40: 16000000 INFO @ Sat, 15 Jan 2022 17:51:41: 11000000 INFO @ Sat, 15 Jan 2022 17:51:46: 7000000 INFO @ Sat, 15 Jan 2022 17:51:46: 17000000 INFO @ Sat, 15 Jan 2022 17:51:47: 12000000 INFO @ Sat, 15 Jan 2022 17:51:51: 18000000 INFO @ Sat, 15 Jan 2022 17:51:51: 8000000 INFO @ Sat, 15 Jan 2022 17:51:54: 13000000 INFO @ Sat, 15 Jan 2022 17:51:57: 19000000 INFO @ Sat, 15 Jan 2022 17:51:57: 9000000 INFO @ Sat, 15 Jan 2022 17:52:00: 14000000 INFO @ Sat, 15 Jan 2022 17:52:03: 20000000 INFO @ Sat, 15 Jan 2022 17:52:04: 10000000 INFO @ Sat, 15 Jan 2022 17:52:06: 15000000 INFO @ Sat, 15 Jan 2022 17:52:08: 21000000 INFO @ Sat, 15 Jan 2022 17:52:10: 11000000 INFO @ Sat, 15 Jan 2022 17:52:12: 16000000 INFO @ Sat, 15 Jan 2022 17:52:14: 22000000 INFO @ Sat, 15 Jan 2022 17:52:16: 12000000 INFO @ Sat, 15 Jan 2022 17:52:18: 17000000 INFO @ Sat, 15 Jan 2022 17:52:19: 23000000 INFO @ Sat, 15 Jan 2022 17:52:22: 13000000 INFO @ Sat, 15 Jan 2022 17:52:24: 18000000 INFO @ Sat, 15 Jan 2022 17:52:24: 24000000 INFO @ Sat, 15 Jan 2022 17:52:28: 14000000 INFO @ Sat, 15 Jan 2022 17:52:30: 25000000 INFO @ Sat, 15 Jan 2022 17:52:30: 19000000 INFO @ Sat, 15 Jan 2022 17:52:34: 15000000 INFO @ Sat, 15 Jan 2022 17:52:35: 26000000 INFO @ Sat, 15 Jan 2022 17:52:37: 20000000 INFO @ Sat, 15 Jan 2022 17:52:40: 16000000 INFO @ Sat, 15 Jan 2022 17:52:40: 27000000 INFO @ Sat, 15 Jan 2022 17:52:43: 21000000 INFO @ Sat, 15 Jan 2022 17:52:46: 17000000 INFO @ Sat, 15 Jan 2022 17:52:46: 28000000 INFO @ Sat, 15 Jan 2022 17:52:49: 22000000 INFO @ Sat, 15 Jan 2022 17:52:51: 29000000 INFO @ Sat, 15 Jan 2022 17:52:51: 18000000 INFO @ Sat, 15 Jan 2022 17:52:55: 23000000 INFO @ Sat, 15 Jan 2022 17:52:56: 30000000 INFO @ Sat, 15 Jan 2022 17:52:57: 19000000 INFO @ Sat, 15 Jan 2022 17:53:01: 24000000 INFO @ Sat, 15 Jan 2022 17:53:02: 31000000 INFO @ Sat, 15 Jan 2022 17:53:03: 20000000 INFO @ Sat, 15 Jan 2022 17:53:07: 32000000 INFO @ Sat, 15 Jan 2022 17:53:07: 25000000 INFO @ Sat, 15 Jan 2022 17:53:09: 21000000 INFO @ Sat, 15 Jan 2022 17:53:12: 33000000 INFO @ Sat, 15 Jan 2022 17:53:14: 26000000 INFO @ Sat, 15 Jan 2022 17:53:15: 22000000 INFO @ Sat, 15 Jan 2022 17:53:18: 34000000 INFO @ Sat, 15 Jan 2022 17:53:20: 27000000 INFO @ Sat, 15 Jan 2022 17:53:21: 23000000 INFO @ Sat, 15 Jan 2022 17:53:23: 35000000 INFO @ Sat, 15 Jan 2022 17:53:26: 28000000 INFO @ Sat, 15 Jan 2022 17:53:27: 24000000 INFO @ Sat, 15 Jan 2022 17:53:28: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:53:32: 29000000 INFO @ Sat, 15 Jan 2022 17:53:33: 25000000 INFO @ Sat, 15 Jan 2022 17:53:34: 37000000 INFO @ Sat, 15 Jan 2022 17:53:38: 30000000 INFO @ Sat, 15 Jan 2022 17:53:39: 38000000 INFO @ Sat, 15 Jan 2022 17:53:39: 26000000 INFO @ Sat, 15 Jan 2022 17:53:44: 39000000 INFO @ Sat, 15 Jan 2022 17:53:44: 31000000 INFO @ Sat, 15 Jan 2022 17:53:46: 27000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:53:50: 40000000 INFO @ Sat, 15 Jan 2022 17:53:51: 32000000 INFO @ Sat, 15 Jan 2022 17:53:52: 28000000 INFO @ Sat, 15 Jan 2022 17:53:55: 41000000 INFO @ Sat, 15 Jan 2022 17:53:57: 33000000 INFO @ Sat, 15 Jan 2022 17:53:58: 29000000 INFO @ Sat, 15 Jan 2022 17:54:00: 42000000 INFO @ Sat, 15 Jan 2022 17:54:03: 34000000 INFO @ Sat, 15 Jan 2022 17:54:04: 30000000 INFO @ Sat, 15 Jan 2022 17:54:05: 43000000 INFO @ Sat, 15 Jan 2022 17:54:09: 35000000 INFO @ Sat, 15 Jan 2022 17:54:10: 31000000 INFO @ Sat, 15 Jan 2022 17:54:11: 44000000 INFO @ Sat, 15 Jan 2022 17:54:15: 36000000 INFO @ Sat, 15 Jan 2022 17:54:16: 45000000 INFO @ Sat, 15 Jan 2022 17:54:16: 32000000 INFO @ Sat, 15 Jan 2022 17:54:21: 37000000 INFO @ Sat, 15 Jan 2022 17:54:21: 46000000 INFO @ Sat, 15 Jan 2022 17:54:22: 33000000 INFO @ Sat, 15 Jan 2022 17:54:27: 47000000 INFO @ Sat, 15 Jan 2022 17:54:27: 38000000 INFO @ Sat, 15 Jan 2022 17:54:28: 34000000 INFO @ Sat, 15 Jan 2022 17:54:32: 48000000 INFO @ Sat, 15 Jan 2022 17:54:33: 39000000 INFO @ Sat, 15 Jan 2022 17:54:34: 35000000 INFO @ Sat, 15 Jan 2022 17:54:37: 49000000 INFO @ Sat, 15 Jan 2022 17:54:40: 40000000 INFO @ Sat, 15 Jan 2022 17:54:40: 36000000 INFO @ Sat, 15 Jan 2022 17:54:43: 50000000 INFO @ Sat, 15 Jan 2022 17:54:46: 41000000 INFO @ Sat, 15 Jan 2022 17:54:47: 37000000 INFO @ Sat, 15 Jan 2022 17:54:49: 51000000 INFO @ Sat, 15 Jan 2022 17:54:52: 42000000 INFO @ Sat, 15 Jan 2022 17:54:53: 38000000 INFO @ Sat, 15 Jan 2022 17:54:55: 52000000 INFO @ Sat, 15 Jan 2022 17:54:58: 43000000 INFO @ Sat, 15 Jan 2022 17:54:59: 39000000 INFO @ Sat, 15 Jan 2022 17:55:00: 53000000 INFO @ Sat, 15 Jan 2022 17:55:04: 44000000 INFO @ Sat, 15 Jan 2022 17:55:05: 40000000 INFO @ Sat, 15 Jan 2022 17:55:06: 54000000 INFO @ Sat, 15 Jan 2022 17:55:10: 45000000 INFO @ Sat, 15 Jan 2022 17:55:11: 41000000 INFO @ Sat, 15 Jan 2022 17:55:12: 55000000 INFO @ Sat, 15 Jan 2022 17:55:17: 46000000 INFO @ Sat, 15 Jan 2022 17:55:17: 56000000 INFO @ Sat, 15 Jan 2022 17:55:17: 42000000 INFO @ Sat, 15 Jan 2022 17:55:20: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:55:20: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:55:20: #1 total tags in treatment: 26947829 INFO @ Sat, 15 Jan 2022 17:55:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:55:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:55:20: #1 tags after filtering in treatment: 14620616 INFO @ Sat, 15 Jan 2022 17:55:20: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 17:55:20: #1 finished! INFO @ Sat, 15 Jan 2022 17:55:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:55:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:55:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:55:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:55:23: 47000000 INFO @ Sat, 15 Jan 2022 17:55:23: 43000000 INFO @ Sat, 15 Jan 2022 17:55:29: 48000000 INFO @ Sat, 15 Jan 2022 17:55:29: 44000000 INFO @ Sat, 15 Jan 2022 17:55:35: 49000000 INFO @ Sat, 15 Jan 2022 17:55:35: 45000000 INFO @ Sat, 15 Jan 2022 17:55:41: 50000000 INFO @ Sat, 15 Jan 2022 17:55:42: 46000000 INFO @ Sat, 15 Jan 2022 17:55:47: 51000000 INFO @ Sat, 15 Jan 2022 17:55:48: 47000000 INFO @ Sat, 15 Jan 2022 17:55:53: 52000000 INFO @ Sat, 15 Jan 2022 17:55:54: 48000000 INFO @ Sat, 15 Jan 2022 17:55:59: 53000000 INFO @ Sat, 15 Jan 2022 17:56:00: 49000000 INFO @ Sat, 15 Jan 2022 17:56:05: 54000000 INFO @ Sat, 15 Jan 2022 17:56:06: 50000000 INFO @ Sat, 15 Jan 2022 17:56:10: 55000000 INFO @ Sat, 15 Jan 2022 17:56:12: 51000000 INFO @ Sat, 15 Jan 2022 17:56:16: 56000000 INFO @ Sat, 15 Jan 2022 17:56:18: 52000000 INFO @ Sat, 15 Jan 2022 17:56:19: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:56:19: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:56:19: #1 total tags in treatment: 26947829 INFO @ Sat, 15 Jan 2022 17:56:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:56:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:56:20: #1 tags after filtering in treatment: 14620616 INFO @ Sat, 15 Jan 2022 17:56:20: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 17:56:20: #1 finished! INFO @ Sat, 15 Jan 2022 17:56:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:56:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:56:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:56:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:56:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:56:23: 53000000 INFO @ Sat, 15 Jan 2022 17:56:29: 54000000 INFO @ Sat, 15 Jan 2022 17:56:34: 55000000 INFO @ Sat, 15 Jan 2022 17:56:42: 56000000 INFO @ Sat, 15 Jan 2022 17:56:45: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 17:56:45: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 17:56:45: #1 total tags in treatment: 26947829 INFO @ Sat, 15 Jan 2022 17:56:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:56:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:56:45: #1 tags after filtering in treatment: 14620616 INFO @ Sat, 15 Jan 2022 17:56:45: #1 Redundant rate of treatment: 0.46 INFO @ Sat, 15 Jan 2022 17:56:45: #1 finished! INFO @ Sat, 15 Jan 2022 17:56:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:56:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:56:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:56:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:56:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973302/SRX7973302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling