Job ID = 14519968 SRX = SRX7973299 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26436298 spots for SRR11394482/SRR11394482.sra Written 26436298 spots for SRR11394482/SRR11394482.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:29 26436298 reads; of these: 26436298 (100.00%) were paired; of these: 10475330 (39.62%) aligned concordantly 0 times 12802361 (48.43%) aligned concordantly exactly 1 time 3158607 (11.95%) aligned concordantly >1 times ---- 10475330 pairs aligned concordantly 0 times; of these: 413040 (3.94%) aligned discordantly 1 time ---- 10062290 pairs aligned 0 times concordantly or discordantly; of these: 20124580 mates make up the pairs; of these: 19627839 (97.53%) aligned 0 times 201334 (1.00%) aligned exactly 1 time 295407 (1.47%) aligned >1 times 62.88% overall alignment rate Time searching: 00:08:29 Overall time: 00:08:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1036256 / 16368834 = 0.0633 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:26:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:26:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:26:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:26:41: 1000000 INFO @ Sat, 15 Jan 2022 18:26:47: 2000000 INFO @ Sat, 15 Jan 2022 18:26:52: 3000000 INFO @ Sat, 15 Jan 2022 18:26:57: 4000000 INFO @ Sat, 15 Jan 2022 18:27:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:06: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:06: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:07: 6000000 INFO @ Sat, 15 Jan 2022 18:27:11: 1000000 INFO @ Sat, 15 Jan 2022 18:27:13: 7000000 INFO @ Sat, 15 Jan 2022 18:27:16: 2000000 INFO @ Sat, 15 Jan 2022 18:27:19: 8000000 INFO @ Sat, 15 Jan 2022 18:27:22: 3000000 INFO @ Sat, 15 Jan 2022 18:27:24: 9000000 INFO @ Sat, 15 Jan 2022 18:27:27: 4000000 INFO @ Sat, 15 Jan 2022 18:27:30: 10000000 INFO @ Sat, 15 Jan 2022 18:27:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:36: 11000000 INFO @ Sat, 15 Jan 2022 18:27:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:36: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:36: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:37: 6000000 INFO @ Sat, 15 Jan 2022 18:27:41: 12000000 INFO @ Sat, 15 Jan 2022 18:27:42: 1000000 INFO @ Sat, 15 Jan 2022 18:27:42: 7000000 INFO @ Sat, 15 Jan 2022 18:27:47: 13000000 INFO @ Sat, 15 Jan 2022 18:27:47: 8000000 INFO @ Sat, 15 Jan 2022 18:27:48: 2000000 INFO @ Sat, 15 Jan 2022 18:27:52: 9000000 INFO @ Sat, 15 Jan 2022 18:27:53: 14000000 INFO @ Sat, 15 Jan 2022 18:27:53: 3000000 INFO @ Sat, 15 Jan 2022 18:27:58: 10000000 INFO @ Sat, 15 Jan 2022 18:27:59: 15000000 INFO @ Sat, 15 Jan 2022 18:27:59: 4000000 INFO @ Sat, 15 Jan 2022 18:28:03: 11000000 INFO @ Sat, 15 Jan 2022 18:28:04: 16000000 INFO @ Sat, 15 Jan 2022 18:28:05: 5000000 INFO @ Sat, 15 Jan 2022 18:28:08: 12000000 INFO @ Sat, 15 Jan 2022 18:28:10: 17000000 INFO @ Sat, 15 Jan 2022 18:28:11: 6000000 INFO @ Sat, 15 Jan 2022 18:28:14: 13000000 INFO @ Sat, 15 Jan 2022 18:28:16: 18000000 INFO @ Sat, 15 Jan 2022 18:28:17: 7000000 INFO @ Sat, 15 Jan 2022 18:28:19: 14000000 INFO @ Sat, 15 Jan 2022 18:28:22: 19000000 INFO @ Sat, 15 Jan 2022 18:28:22: 8000000 INFO @ Sat, 15 Jan 2022 18:28:24: 15000000 INFO @ Sat, 15 Jan 2022 18:28:27: 20000000 INFO @ Sat, 15 Jan 2022 18:28:28: 9000000 INFO @ Sat, 15 Jan 2022 18:28:29: 16000000 INFO @ Sat, 15 Jan 2022 18:28:33: 21000000 INFO @ Sat, 15 Jan 2022 18:28:34: 10000000 INFO @ Sat, 15 Jan 2022 18:28:34: 17000000 INFO @ Sat, 15 Jan 2022 18:28:39: 22000000 INFO @ Sat, 15 Jan 2022 18:28:39: 18000000 INFO @ Sat, 15 Jan 2022 18:28:39: 11000000 INFO @ Sat, 15 Jan 2022 18:28:44: 19000000 INFO @ Sat, 15 Jan 2022 18:28:45: 23000000 INFO @ Sat, 15 Jan 2022 18:28:45: 12000000 INFO @ Sat, 15 Jan 2022 18:28:50: 20000000 INFO @ Sat, 15 Jan 2022 18:28:50: 24000000 INFO @ Sat, 15 Jan 2022 18:28:51: 13000000 INFO @ Sat, 15 Jan 2022 18:28:55: 21000000 INFO @ Sat, 15 Jan 2022 18:28:56: 25000000 INFO @ Sat, 15 Jan 2022 18:28:57: 14000000 INFO @ Sat, 15 Jan 2022 18:29:00: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:29:02: 26000000 INFO @ Sat, 15 Jan 2022 18:29:02: 15000000 INFO @ Sat, 15 Jan 2022 18:29:05: 23000000 INFO @ Sat, 15 Jan 2022 18:29:08: 27000000 INFO @ Sat, 15 Jan 2022 18:29:08: 16000000 INFO @ Sat, 15 Jan 2022 18:29:10: 24000000 INFO @ Sat, 15 Jan 2022 18:29:13: 28000000 INFO @ Sat, 15 Jan 2022 18:29:14: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:29:15: 25000000 INFO @ Sat, 15 Jan 2022 18:29:19: 29000000 INFO @ Sat, 15 Jan 2022 18:29:20: 18000000 INFO @ Sat, 15 Jan 2022 18:29:20: 26000000 INFO @ Sat, 15 Jan 2022 18:29:25: 30000000 INFO @ Sat, 15 Jan 2022 18:29:25: 19000000 INFO @ Sat, 15 Jan 2022 18:29:26: 27000000 INFO @ Sat, 15 Jan 2022 18:29:31: 28000000 INFO @ Sat, 15 Jan 2022 18:29:31: 31000000 INFO @ Sat, 15 Jan 2022 18:29:31: 20000000 INFO @ Sat, 15 Jan 2022 18:29:32: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 18:29:32: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 18:29:32: #1 total tags in treatment: 14935296 INFO @ Sat, 15 Jan 2022 18:29:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:32: #1 tags after filtering in treatment: 9969118 INFO @ Sat, 15 Jan 2022 18:29:32: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 18:29:32: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:29:36: 29000000 INFO @ Sat, 15 Jan 2022 18:29:37: 21000000 INFO @ Sat, 15 Jan 2022 18:29:41: 30000000 INFO @ Sat, 15 Jan 2022 18:29:42: 22000000 INFO @ Sat, 15 Jan 2022 18:29:46: 31000000 INFO @ Sat, 15 Jan 2022 18:29:47: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 18:29:47: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 18:29:47: #1 total tags in treatment: 14935296 INFO @ Sat, 15 Jan 2022 18:29:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:47: #1 tags after filtering in treatment: 9969118 INFO @ Sat, 15 Jan 2022 18:29:47: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 18:29:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:48: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:48: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 18:29:48: 23000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:29:53: 24000000 INFO @ Sat, 15 Jan 2022 18:29:59: 25000000 INFO @ Sat, 15 Jan 2022 18:30:04: 26000000 INFO @ Sat, 15 Jan 2022 18:30:09: 27000000 INFO @ Sat, 15 Jan 2022 18:30:14: 28000000 INFO @ Sat, 15 Jan 2022 18:30:20: 29000000 INFO @ Sat, 15 Jan 2022 18:30:25: 30000000 INFO @ Sat, 15 Jan 2022 18:30:30: 31000000 INFO @ Sat, 15 Jan 2022 18:30:31: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 18:30:31: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 18:30:31: #1 total tags in treatment: 14935296 INFO @ Sat, 15 Jan 2022 18:30:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:31: #1 tags after filtering in treatment: 9969118 INFO @ Sat, 15 Jan 2022 18:30:31: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 18:30:31: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7973299/SRX7973299.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling