Job ID = 7108164 SRX = SRX7960937 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 41452168 spots for SRR11359442/SRR11359442.sra Written 41452168 spots for SRR11359442/SRR11359442.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:00 41452168 reads; of these: 41452168 (100.00%) were unpaired; of these: 3411372 (8.23%) aligned 0 times 27435978 (66.19%) aligned exactly 1 time 10604818 (25.58%) aligned >1 times 91.77% overall alignment rate Time searching: 00:16:00 Overall time: 00:16:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 28126440 / 38040796 = 0.7394 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:58:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:58:38: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:58:38: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:58:44: 1000000 INFO @ Wed, 22 Jul 2020 13:58:50: 2000000 INFO @ Wed, 22 Jul 2020 13:58:57: 3000000 INFO @ Wed, 22 Jul 2020 13:59:03: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:59:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:59:07: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:59:07: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:59:10: 5000000 INFO @ Wed, 22 Jul 2020 13:59:15: 1000000 INFO @ Wed, 22 Jul 2020 13:59:17: 6000000 INFO @ Wed, 22 Jul 2020 13:59:23: 2000000 INFO @ Wed, 22 Jul 2020 13:59:24: 7000000 INFO @ Wed, 22 Jul 2020 13:59:31: 8000000 INFO @ Wed, 22 Jul 2020 13:59:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:59:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:59:37: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:59:37: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:59:38: 9000000 INFO @ Wed, 22 Jul 2020 13:59:39: 4000000 INFO @ Wed, 22 Jul 2020 13:59:44: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 13:59:44: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 13:59:44: #1 total tags in treatment: 9914356 INFO @ Wed, 22 Jul 2020 13:59:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:59:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:59:44: #1 tags after filtering in treatment: 9914356 INFO @ Wed, 22 Jul 2020 13:59:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:59:44: #1 finished! INFO @ Wed, 22 Jul 2020 13:59:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:59:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:59:45: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:59:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:59:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:59:45: 1000000 INFO @ Wed, 22 Jul 2020 13:59:47: 5000000 INFO @ Wed, 22 Jul 2020 13:59:53: 2000000 INFO @ Wed, 22 Jul 2020 13:59:55: 6000000 INFO @ Wed, 22 Jul 2020 14:00:02: 3000000 INFO @ Wed, 22 Jul 2020 14:00:03: 7000000 INFO @ Wed, 22 Jul 2020 14:00:10: 4000000 INFO @ Wed, 22 Jul 2020 14:00:11: 8000000 INFO @ Wed, 22 Jul 2020 14:00:18: 5000000 INFO @ Wed, 22 Jul 2020 14:00:19: 9000000 INFO @ Wed, 22 Jul 2020 14:00:26: 6000000 INFO @ Wed, 22 Jul 2020 14:00:26: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 14:00:26: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 14:00:26: #1 total tags in treatment: 9914356 INFO @ Wed, 22 Jul 2020 14:00:26: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:00:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:00:27: #1 tags after filtering in treatment: 9914356 INFO @ Wed, 22 Jul 2020 14:00:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 14:00:27: #1 finished! INFO @ Wed, 22 Jul 2020 14:00:27: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:00:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:00:27: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:00:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:00:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 14:00:34: 7000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 14:00:41: 8000000 INFO @ Wed, 22 Jul 2020 14:00:49: 9000000 INFO @ Wed, 22 Jul 2020 14:00:56: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 14:00:56: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 14:00:56: #1 total tags in treatment: 9914356 INFO @ Wed, 22 Jul 2020 14:00:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 14:00:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 14:00:57: #1 tags after filtering in treatment: 9914356 INFO @ Wed, 22 Jul 2020 14:00:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 14:00:57: #1 finished! INFO @ Wed, 22 Jul 2020 14:00:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 14:00:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 14:00:57: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 14:00:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 14:00:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960937/SRX7960937.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling