Job ID = 7108121 SRX = SRX7960933 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 34548467 spots for SRR11359438/SRR11359438.sra Written 34548467 spots for SRR11359438/SRR11359438.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:50 34548467 reads; of these: 34548467 (100.00%) were unpaired; of these: 2820346 (8.16%) aligned 0 times 27166947 (78.63%) aligned exactly 1 time 4561174 (13.20%) aligned >1 times 91.84% overall alignment rate Time searching: 00:12:50 Overall time: 00:12:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 20975603 / 31728121 = 0.6611 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:52:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:52:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:52:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:52:30: 1000000 INFO @ Wed, 22 Jul 2020 13:52:37: 2000000 INFO @ Wed, 22 Jul 2020 13:52:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:52:52: 4000000 INFO @ Wed, 22 Jul 2020 13:52:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:52:53: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:52:53: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:52:59: 5000000 INFO @ Wed, 22 Jul 2020 13:53:02: 1000000 INFO @ Wed, 22 Jul 2020 13:53:07: 6000000 INFO @ Wed, 22 Jul 2020 13:53:11: 2000000 INFO @ Wed, 22 Jul 2020 13:53:15: 7000000 INFO @ Wed, 22 Jul 2020 13:53:20: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 13:53:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 13:53:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 13:53:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 13:53:23: 8000000 INFO @ Wed, 22 Jul 2020 13:53:29: 4000000 INFO @ Wed, 22 Jul 2020 13:53:31: 9000000 INFO @ Wed, 22 Jul 2020 13:53:31: 1000000 INFO @ Wed, 22 Jul 2020 13:53:38: 5000000 INFO @ Wed, 22 Jul 2020 13:53:39: 10000000 INFO @ Wed, 22 Jul 2020 13:53:39: 2000000 INFO @ Wed, 22 Jul 2020 13:53:45: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 13:53:45: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 13:53:45: #1 total tags in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:53:45: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:53:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:53:45: #1 tags after filtering in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:53:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:53:45: #1 finished! INFO @ Wed, 22 Jul 2020 13:53:45: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:53:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:53:46: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:53:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:53:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:53:47: 6000000 INFO @ Wed, 22 Jul 2020 13:53:47: 3000000 INFO @ Wed, 22 Jul 2020 13:53:56: 4000000 INFO @ Wed, 22 Jul 2020 13:53:56: 7000000 INFO @ Wed, 22 Jul 2020 13:54:04: 5000000 INFO @ Wed, 22 Jul 2020 13:54:05: 8000000 INFO @ Wed, 22 Jul 2020 13:54:12: 6000000 INFO @ Wed, 22 Jul 2020 13:54:14: 9000000 INFO @ Wed, 22 Jul 2020 13:54:20: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 13:54:23: 10000000 INFO @ Wed, 22 Jul 2020 13:54:28: 8000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 13:54:30: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 13:54:30: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 13:54:30: #1 total tags in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:54:30: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:54:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:54:30: #1 tags after filtering in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:54:30: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:54:30: #1 finished! INFO @ Wed, 22 Jul 2020 13:54:30: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:54:30: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:54:31: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:54:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:54:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 13:54:35: 9000000 INFO @ Wed, 22 Jul 2020 13:54:43: 10000000 INFO @ Wed, 22 Jul 2020 13:54:48: #1 tag size is determined as 151 bps INFO @ Wed, 22 Jul 2020 13:54:48: #1 tag size = 151 INFO @ Wed, 22 Jul 2020 13:54:48: #1 total tags in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:54:48: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 13:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 13:54:48: #1 tags after filtering in treatment: 10752518 INFO @ Wed, 22 Jul 2020 13:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 13:54:48: #1 finished! INFO @ Wed, 22 Jul 2020 13:54:48: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 13:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 13:54:49: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 13:54:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 13:54:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7960933/SRX7960933.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling