Job ID = 14520219 SRX = SRX7919612 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16266885 spots for SRR11315229/SRR11315229.sra Written 16266885 spots for SRR11315229/SRR11315229.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:19 16266885 reads; of these: 16266885 (100.00%) were paired; of these: 1486831 (9.14%) aligned concordantly 0 times 13538703 (83.23%) aligned concordantly exactly 1 time 1241351 (7.63%) aligned concordantly >1 times ---- 1486831 pairs aligned concordantly 0 times; of these: 72909 (4.90%) aligned discordantly 1 time ---- 1413922 pairs aligned 0 times concordantly or discordantly; of these: 2827844 mates make up the pairs; of these: 1884314 (66.63%) aligned 0 times 817756 (28.92%) aligned exactly 1 time 125774 (4.45%) aligned >1 times 94.21% overall alignment rate Time searching: 00:13:19 Overall time: 00:13:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2481957 / 14810221 = 0.1676 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:48: 1000000 INFO @ Sat, 15 Jan 2022 19:04:55: 2000000 INFO @ Sat, 15 Jan 2022 19:05:02: 3000000 INFO @ Sat, 15 Jan 2022 19:05:09: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:17: 5000000 INFO @ Sat, 15 Jan 2022 19:05:19: 1000000 INFO @ Sat, 15 Jan 2022 19:05:24: 6000000 INFO @ Sat, 15 Jan 2022 19:05:26: 2000000 INFO @ Sat, 15 Jan 2022 19:05:31: 7000000 INFO @ Sat, 15 Jan 2022 19:05:34: 3000000 INFO @ Sat, 15 Jan 2022 19:05:39: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:41: 4000000 INFO @ Sat, 15 Jan 2022 19:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:42: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:42: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:46: 9000000 INFO @ Sat, 15 Jan 2022 19:05:49: 1000000 INFO @ Sat, 15 Jan 2022 19:05:49: 5000000 INFO @ Sat, 15 Jan 2022 19:05:54: 10000000 INFO @ Sat, 15 Jan 2022 19:05:56: 2000000 INFO @ Sat, 15 Jan 2022 19:05:57: 6000000 INFO @ Sat, 15 Jan 2022 19:06:02: 11000000 INFO @ Sat, 15 Jan 2022 19:06:03: 3000000 INFO @ Sat, 15 Jan 2022 19:06:04: 7000000 INFO @ Sat, 15 Jan 2022 19:06:09: 12000000 INFO @ Sat, 15 Jan 2022 19:06:10: 4000000 INFO @ Sat, 15 Jan 2022 19:06:12: 8000000 INFO @ Sat, 15 Jan 2022 19:06:17: 13000000 INFO @ Sat, 15 Jan 2022 19:06:17: 5000000 INFO @ Sat, 15 Jan 2022 19:06:19: 9000000 INFO @ Sat, 15 Jan 2022 19:06:24: 6000000 INFO @ Sat, 15 Jan 2022 19:06:24: 14000000 INFO @ Sat, 15 Jan 2022 19:06:27: 10000000 INFO @ Sat, 15 Jan 2022 19:06:30: 7000000 INFO @ Sat, 15 Jan 2022 19:06:32: 15000000 INFO @ Sat, 15 Jan 2022 19:06:34: 11000000 INFO @ Sat, 15 Jan 2022 19:06:37: 8000000 INFO @ Sat, 15 Jan 2022 19:06:39: 16000000 INFO @ Sat, 15 Jan 2022 19:06:42: 12000000 INFO @ Sat, 15 Jan 2022 19:06:43: 9000000 INFO @ Sat, 15 Jan 2022 19:06:47: 17000000 INFO @ Sat, 15 Jan 2022 19:06:49: 13000000 INFO @ Sat, 15 Jan 2022 19:06:50: 10000000 INFO @ Sat, 15 Jan 2022 19:06:54: 18000000 INFO @ Sat, 15 Jan 2022 19:06:56: 11000000 INFO @ Sat, 15 Jan 2022 19:06:56: 14000000 INFO @ Sat, 15 Jan 2022 19:07:01: 19000000 INFO @ Sat, 15 Jan 2022 19:07:02: 12000000 INFO @ Sat, 15 Jan 2022 19:07:04: 15000000 INFO @ Sat, 15 Jan 2022 19:07:08: 20000000 INFO @ Sat, 15 Jan 2022 19:07:09: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:07:11: 16000000 INFO @ Sat, 15 Jan 2022 19:07:15: 14000000 INFO @ Sat, 15 Jan 2022 19:07:16: 21000000 INFO @ Sat, 15 Jan 2022 19:07:18: 17000000 INFO @ Sat, 15 Jan 2022 19:07:21: 15000000 INFO @ Sat, 15 Jan 2022 19:07:23: 22000000 INFO @ Sat, 15 Jan 2022 19:07:25: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:07:27: 16000000 INFO @ Sat, 15 Jan 2022 19:07:30: 23000000 INFO @ Sat, 15 Jan 2022 19:07:32: 19000000 INFO @ Sat, 15 Jan 2022 19:07:34: 17000000 INFO @ Sat, 15 Jan 2022 19:07:37: 24000000 INFO @ Sat, 15 Jan 2022 19:07:39: 20000000 INFO @ Sat, 15 Jan 2022 19:07:40: 18000000 INFO @ Sat, 15 Jan 2022 19:07:44: 25000000 INFO @ Sat, 15 Jan 2022 19:07:46: 19000000 INFO @ Sat, 15 Jan 2022 19:07:47: 21000000 INFO @ Sat, 15 Jan 2022 19:07:49: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:07:49: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:07:49: #1 total tags in treatment: 12302461 INFO @ Sat, 15 Jan 2022 19:07:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:07:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:07:49: #1 tags after filtering in treatment: 7867392 INFO @ Sat, 15 Jan 2022 19:07:49: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:07:49: #1 finished! INFO @ Sat, 15 Jan 2022 19:07:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:07:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:07:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:07:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:07:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:07:52: 20000000 INFO @ Sat, 15 Jan 2022 19:07:53: 22000000 INFO @ Sat, 15 Jan 2022 19:07:59: 21000000 INFO @ Sat, 15 Jan 2022 19:08:00: 23000000 INFO @ Sat, 15 Jan 2022 19:08:05: 22000000 INFO @ Sat, 15 Jan 2022 19:08:07: 24000000 INFO @ Sat, 15 Jan 2022 19:08:11: 23000000 INFO @ Sat, 15 Jan 2022 19:08:14: 25000000 INFO @ Sat, 15 Jan 2022 19:08:17: 24000000 INFO @ Sat, 15 Jan 2022 19:08:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:08:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:08:19: #1 total tags in treatment: 12302461 INFO @ Sat, 15 Jan 2022 19:08:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:19: #1 tags after filtering in treatment: 7867392 INFO @ Sat, 15 Jan 2022 19:08:19: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:08:19: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:08:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:08:23: 25000000 INFO @ Sat, 15 Jan 2022 19:08:27: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:08:27: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:08:27: #1 total tags in treatment: 12302461 INFO @ Sat, 15 Jan 2022 19:08:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:08:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:08:28: #1 tags after filtering in treatment: 7867392 INFO @ Sat, 15 Jan 2022 19:08:28: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:08:28: #1 finished! INFO @ Sat, 15 Jan 2022 19:08:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:08:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:08:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:08:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:08:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919612/SRX7919612.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling