Job ID = 14520204 SRX = SRX7919608 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18397217 spots for SRR11315225/SRR11315225.sra Written 18397217 spots for SRR11315225/SRR11315225.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:01 18397217 reads; of these: 18397217 (100.00%) were paired; of these: 1204378 (6.55%) aligned concordantly 0 times 15376430 (83.58%) aligned concordantly exactly 1 time 1816409 (9.87%) aligned concordantly >1 times ---- 1204378 pairs aligned concordantly 0 times; of these: 262591 (21.80%) aligned discordantly 1 time ---- 941787 pairs aligned 0 times concordantly or discordantly; of these: 1883574 mates make up the pairs; of these: 1536288 (81.56%) aligned 0 times 245902 (13.06%) aligned exactly 1 time 101384 (5.38%) aligned >1 times 95.82% overall alignment rate Time searching: 00:10:01 Overall time: 00:10:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4235045 / 17215359 = 0.2460 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:55:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:55:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:55:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:55:59: 1000000 INFO @ Sat, 15 Jan 2022 18:56:04: 2000000 INFO @ Sat, 15 Jan 2022 18:56:09: 3000000 INFO @ Sat, 15 Jan 2022 18:56:14: 4000000 INFO @ Sat, 15 Jan 2022 18:56:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:24: 6000000 INFO @ Sat, 15 Jan 2022 18:56:29: 1000000 INFO @ Sat, 15 Jan 2022 18:56:30: 7000000 INFO @ Sat, 15 Jan 2022 18:56:35: 2000000 INFO @ Sat, 15 Jan 2022 18:56:35: 8000000 INFO @ Sat, 15 Jan 2022 18:56:41: 3000000 INFO @ Sat, 15 Jan 2022 18:56:41: 9000000 INFO @ Sat, 15 Jan 2022 18:56:46: 4000000 INFO @ Sat, 15 Jan 2022 18:56:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:56:52: 11000000 INFO @ Sat, 15 Jan 2022 18:56:52: 5000000 INFO @ Sat, 15 Jan 2022 18:56:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:56:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:56:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:56:58: 12000000 INFO @ Sat, 15 Jan 2022 18:56:58: 6000000 INFO @ Sat, 15 Jan 2022 18:57:00: 1000000 INFO @ Sat, 15 Jan 2022 18:57:05: 13000000 INFO @ Sat, 15 Jan 2022 18:57:05: 7000000 INFO @ Sat, 15 Jan 2022 18:57:07: 2000000 INFO @ Sat, 15 Jan 2022 18:57:11: 14000000 INFO @ Sat, 15 Jan 2022 18:57:11: 8000000 INFO @ Sat, 15 Jan 2022 18:57:14: 3000000 INFO @ Sat, 15 Jan 2022 18:57:17: 15000000 INFO @ Sat, 15 Jan 2022 18:57:17: 9000000 INFO @ Sat, 15 Jan 2022 18:57:21: 4000000 INFO @ Sat, 15 Jan 2022 18:57:23: 16000000 INFO @ Sat, 15 Jan 2022 18:57:24: 10000000 INFO @ Sat, 15 Jan 2022 18:57:28: 5000000 INFO @ Sat, 15 Jan 2022 18:57:29: 17000000 INFO @ Sat, 15 Jan 2022 18:57:30: 11000000 INFO @ Sat, 15 Jan 2022 18:57:35: 6000000 INFO @ Sat, 15 Jan 2022 18:57:36: 18000000 INFO @ Sat, 15 Jan 2022 18:57:37: 12000000 INFO @ Sat, 15 Jan 2022 18:57:42: 19000000 INFO @ Sat, 15 Jan 2022 18:57:42: 7000000 INFO @ Sat, 15 Jan 2022 18:57:43: 13000000 INFO @ Sat, 15 Jan 2022 18:57:48: 20000000 INFO @ Sat, 15 Jan 2022 18:57:49: 8000000 INFO @ Sat, 15 Jan 2022 18:57:49: 14000000 INFO @ Sat, 15 Jan 2022 18:57:55: 21000000 INFO @ Sat, 15 Jan 2022 18:57:55: 15000000 INFO @ Sat, 15 Jan 2022 18:57:56: 9000000 INFO @ Sat, 15 Jan 2022 18:58:01: 22000000 INFO @ Sat, 15 Jan 2022 18:58:02: 16000000 INFO @ Sat, 15 Jan 2022 18:58:03: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:58:07: 23000000 INFO @ Sat, 15 Jan 2022 18:58:08: 17000000 INFO @ Sat, 15 Jan 2022 18:58:10: 11000000 INFO @ Sat, 15 Jan 2022 18:58:13: 24000000 INFO @ Sat, 15 Jan 2022 18:58:14: 18000000 INFO @ Sat, 15 Jan 2022 18:58:16: 12000000 INFO @ Sat, 15 Jan 2022 18:58:20: 25000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:58:21: 19000000 INFO @ Sat, 15 Jan 2022 18:58:23: 13000000 INFO @ Sat, 15 Jan 2022 18:58:26: 26000000 INFO @ Sat, 15 Jan 2022 18:58:27: 20000000 INFO @ Sat, 15 Jan 2022 18:58:30: 14000000 INFO @ Sat, 15 Jan 2022 18:58:31: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:58:31: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:58:31: #1 total tags in treatment: 12961195 INFO @ Sat, 15 Jan 2022 18:58:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:58:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:58:31: #1 tags after filtering in treatment: 6037089 INFO @ Sat, 15 Jan 2022 18:58:31: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:58:31: #1 finished! INFO @ Sat, 15 Jan 2022 18:58:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:58:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:58:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:58:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:58:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:58:33: 21000000 INFO @ Sat, 15 Jan 2022 18:58:37: 15000000 INFO @ Sat, 15 Jan 2022 18:58:39: 22000000 INFO @ Sat, 15 Jan 2022 18:58:44: 16000000 INFO @ Sat, 15 Jan 2022 18:58:46: 23000000 INFO @ Sat, 15 Jan 2022 18:58:51: 17000000 INFO @ Sat, 15 Jan 2022 18:58:52: 24000000 INFO @ Sat, 15 Jan 2022 18:58:57: 18000000 INFO @ Sat, 15 Jan 2022 18:58:58: 25000000 INFO @ Sat, 15 Jan 2022 18:59:04: 19000000 INFO @ Sat, 15 Jan 2022 18:59:04: 26000000 INFO @ Sat, 15 Jan 2022 18:59:09: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:59:09: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:59:09: #1 total tags in treatment: 12961195 INFO @ Sat, 15 Jan 2022 18:59:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:10: #1 tags after filtering in treatment: 6037089 INFO @ Sat, 15 Jan 2022 18:59:10: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:59:10: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:59:11: 20000000 INFO @ Sat, 15 Jan 2022 18:59:17: 21000000 INFO @ Sat, 15 Jan 2022 18:59:23: 22000000 INFO @ Sat, 15 Jan 2022 18:59:29: 23000000 INFO @ Sat, 15 Jan 2022 18:59:35: 24000000 INFO @ Sat, 15 Jan 2022 18:59:41: 25000000 INFO @ Sat, 15 Jan 2022 18:59:47: 26000000 INFO @ Sat, 15 Jan 2022 18:59:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:59:52: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:59:52: #1 total tags in treatment: 12961195 INFO @ Sat, 15 Jan 2022 18:59:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:59:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:59:52: #1 tags after filtering in treatment: 6037089 INFO @ Sat, 15 Jan 2022 18:59:52: #1 Redundant rate of treatment: 0.53 INFO @ Sat, 15 Jan 2022 18:59:52: #1 finished! INFO @ Sat, 15 Jan 2022 18:59:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:59:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:59:52: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:59:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:59:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919608/SRX7919608.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling