Job ID = 14520201 SRX = SRX7919605 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10784533 spots for SRR11315222/SRR11315222.sra Written 10784533 spots for SRR11315222/SRR11315222.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 10784533 reads; of these: 10784533 (100.00%) were paired; of these: 1361740 (12.63%) aligned concordantly 0 times 8674141 (80.43%) aligned concordantly exactly 1 time 748652 (6.94%) aligned concordantly >1 times ---- 1361740 pairs aligned concordantly 0 times; of these: 109764 (8.06%) aligned discordantly 1 time ---- 1251976 pairs aligned 0 times concordantly or discordantly; of these: 2503952 mates make up the pairs; of these: 1764136 (70.45%) aligned 0 times 653671 (26.11%) aligned exactly 1 time 86145 (3.44%) aligned >1 times 91.82% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1567893 / 9435863 = 0.1662 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:47:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:47:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:47:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:47:59: 1000000 INFO @ Sat, 15 Jan 2022 18:48:04: 2000000 INFO @ Sat, 15 Jan 2022 18:48:09: 3000000 INFO @ Sat, 15 Jan 2022 18:48:14: 4000000 INFO @ Sat, 15 Jan 2022 18:48:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:48:24: 6000000 INFO @ Sat, 15 Jan 2022 18:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:48:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:48:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:48:30: 7000000 INFO @ Sat, 15 Jan 2022 18:48:31: 1000000 INFO @ Sat, 15 Jan 2022 18:48:36: 8000000 INFO @ Sat, 15 Jan 2022 18:48:38: 2000000 INFO @ Sat, 15 Jan 2022 18:48:42: 9000000 INFO @ Sat, 15 Jan 2022 18:48:44: 3000000 INFO @ Sat, 15 Jan 2022 18:48:47: 10000000 INFO @ Sat, 15 Jan 2022 18:48:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:48:53: 11000000 INFO @ Sat, 15 Jan 2022 18:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:48:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:48:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:48:55: 5000000 INFO @ Sat, 15 Jan 2022 18:49:00: 12000000 INFO @ Sat, 15 Jan 2022 18:49:01: 1000000 INFO @ Sat, 15 Jan 2022 18:49:02: 6000000 INFO @ Sat, 15 Jan 2022 18:49:06: 13000000 INFO @ Sat, 15 Jan 2022 18:49:09: 7000000 INFO @ Sat, 15 Jan 2022 18:49:09: 2000000 INFO @ Sat, 15 Jan 2022 18:49:13: 14000000 INFO @ Sat, 15 Jan 2022 18:49:15: 8000000 INFO @ Sat, 15 Jan 2022 18:49:16: 3000000 INFO @ Sat, 15 Jan 2022 18:49:19: 15000000 INFO @ Sat, 15 Jan 2022 18:49:22: 9000000 INFO @ Sat, 15 Jan 2022 18:49:23: 4000000 INFO @ Sat, 15 Jan 2022 18:49:26: 16000000 INFO @ Sat, 15 Jan 2022 18:49:28: 10000000 INFO @ Sat, 15 Jan 2022 18:49:30: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:49:30: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:49:30: #1 total tags in treatment: 7856012 INFO @ Sat, 15 Jan 2022 18:49:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:49:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:49:30: #1 tags after filtering in treatment: 4365277 INFO @ Sat, 15 Jan 2022 18:49:30: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:49:30: #1 finished! INFO @ Sat, 15 Jan 2022 18:49:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:49:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:49:30: 5000000 INFO @ Sat, 15 Jan 2022 18:49:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:49:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:49:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:49:35: 11000000 INFO @ Sat, 15 Jan 2022 18:49:37: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:49:41: 12000000 INFO @ Sat, 15 Jan 2022 18:49:45: 7000000 INFO @ Sat, 15 Jan 2022 18:49:47: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:49:52: 8000000 INFO @ Sat, 15 Jan 2022 18:49:54: 14000000 INFO @ Sat, 15 Jan 2022 18:49:59: 9000000 INFO @ Sat, 15 Jan 2022 18:50:00: 15000000 INFO @ Sat, 15 Jan 2022 18:50:06: 10000000 INFO @ Sat, 15 Jan 2022 18:50:06: 16000000 INFO @ Sat, 15 Jan 2022 18:50:11: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:50:11: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:50:11: #1 total tags in treatment: 7856012 INFO @ Sat, 15 Jan 2022 18:50:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:50:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:50:11: #1 tags after filtering in treatment: 4365277 INFO @ Sat, 15 Jan 2022 18:50:11: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:50:11: #1 finished! INFO @ Sat, 15 Jan 2022 18:50:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:50:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:50:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:50:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:50:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:50:13: 11000000 INFO @ Sat, 15 Jan 2022 18:50:19: 12000000 INFO @ Sat, 15 Jan 2022 18:50:26: 13000000 INFO @ Sat, 15 Jan 2022 18:50:32: 14000000 INFO @ Sat, 15 Jan 2022 18:50:39: 15000000 INFO @ Sat, 15 Jan 2022 18:50:45: 16000000 INFO @ Sat, 15 Jan 2022 18:50:49: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:50:49: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:50:49: #1 total tags in treatment: 7856012 INFO @ Sat, 15 Jan 2022 18:50:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:50:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:50:49: #1 tags after filtering in treatment: 4365277 INFO @ Sat, 15 Jan 2022 18:50:49: #1 Redundant rate of treatment: 0.44 INFO @ Sat, 15 Jan 2022 18:50:49: #1 finished! INFO @ Sat, 15 Jan 2022 18:50:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:50:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:50:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:50:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:50:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7919605/SRX7919605.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling