Job ID = 14520138 SRX = SRX7917666 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 11164554 spots for SRR11313219/SRR11313219.sra Written 11164554 spots for SRR11313219/SRR11313219.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:58 11164554 reads; of these: 11164554 (100.00%) were unpaired; of these: 1776794 (15.91%) aligned 0 times 8307936 (74.41%) aligned exactly 1 time 1079824 (9.67%) aligned >1 times 84.09% overall alignment rate Time searching: 00:01:58 Overall time: 00:01:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2211143 / 9387760 = 0.2355 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:54: 1000000 INFO @ Sat, 15 Jan 2022 18:29:02: 2000000 INFO @ Sat, 15 Jan 2022 18:29:10: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:29:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:29:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:29:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:29:18: 4000000 INFO @ Sat, 15 Jan 2022 18:29:25: 1000000 INFO @ Sat, 15 Jan 2022 18:29:27: 5000000 INFO @ Sat, 15 Jan 2022 18:29:33: 2000000 INFO @ Sat, 15 Jan 2022 18:29:35: 6000000 INFO @ Sat, 15 Jan 2022 18:29:42: 3000000 BedGraph に変換中... INFO @ Sat, 15 Jan 2022 18:29:43: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:29:45: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:29:45: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:29:45: #1 total tags in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:29:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:45: #1 tags after filtering in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:29:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:29:45: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:29:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:29:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:29:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:29:51: 4000000 INFO @ Sat, 15 Jan 2022 18:29:55: 1000000 INFO @ Sat, 15 Jan 2022 18:30:00: 5000000 INFO @ Sat, 15 Jan 2022 18:30:03: 2000000 INFO @ Sat, 15 Jan 2022 18:30:09: 6000000 INFO @ Sat, 15 Jan 2022 18:30:11: 3000000 INFO @ Sat, 15 Jan 2022 18:30:18: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:30:19: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:30:19: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:30:19: #1 total tags in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:30:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:19: 4000000 INFO @ Sat, 15 Jan 2022 18:30:20: #1 tags after filtering in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:30:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:30:20: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:30:27: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:30:35: 6000000 INFO @ Sat, 15 Jan 2022 18:30:44: 7000000 INFO @ Sat, 15 Jan 2022 18:30:45: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:30:45: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:30:45: #1 total tags in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:30:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:30:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:30:45: #1 tags after filtering in treatment: 7176617 INFO @ Sat, 15 Jan 2022 18:30:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:30:45: #1 finished! INFO @ Sat, 15 Jan 2022 18:30:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:30:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:30:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:30:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:30:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917666/SRX7917666.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling