Job ID = 14520132 SRX = SRX7917660 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13846818 spots for SRR11313213/SRR11313213.sra Written 13846818 spots for SRR11313213/SRR11313213.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 13846818 reads; of these: 13846818 (100.00%) were unpaired; of these: 562738 (4.06%) aligned 0 times 10728297 (77.48%) aligned exactly 1 time 2555783 (18.46%) aligned >1 times 95.94% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4466089 / 13284080 = 0.3362 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:29:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:29:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:29:49: 1000000 INFO @ Sat, 15 Jan 2022 18:29:58: 2000000 INFO @ Sat, 15 Jan 2022 18:30:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:30:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:30:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:30:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:30:16: 4000000 INFO @ Sat, 15 Jan 2022 18:30:19: 1000000 INFO @ Sat, 15 Jan 2022 18:30:25: 5000000 INFO @ Sat, 15 Jan 2022 18:30:29: 2000000 INFO @ Sat, 15 Jan 2022 18:30:34: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:30:38: 3000000 INFO @ Sat, 15 Jan 2022 18:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:30:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:30:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:30:44: 7000000 INFO @ Sat, 15 Jan 2022 18:30:48: 4000000 INFO @ Sat, 15 Jan 2022 18:30:50: 1000000 INFO @ Sat, 15 Jan 2022 18:30:53: 8000000 INFO @ Sat, 15 Jan 2022 18:30:57: 5000000 INFO @ Sat, 15 Jan 2022 18:31:00: 2000000 INFO @ Sat, 15 Jan 2022 18:31:01: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:31:01: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:31:01: #1 total tags in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:31:01: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:31:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:31:02: #1 tags after filtering in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:31:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:31:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:31:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:31:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:31:02: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:31:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:31:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:31:07: 6000000 INFO @ Sat, 15 Jan 2022 18:31:09: 3000000 INFO @ Sat, 15 Jan 2022 18:31:16: 7000000 INFO @ Sat, 15 Jan 2022 18:31:19: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:31:25: 8000000 INFO @ Sat, 15 Jan 2022 18:31:28: 5000000 INFO @ Sat, 15 Jan 2022 18:31:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:31:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:31:33: #1 total tags in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:31:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:31:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:31:33: #1 tags after filtering in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:31:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:31:33: #1 finished! INFO @ Sat, 15 Jan 2022 18:31:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:31:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:31:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:31:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:31:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:31:37: 6000000 INFO @ Sat, 15 Jan 2022 18:31:46: 7000000 INFO @ Sat, 15 Jan 2022 18:31:55: 8000000 INFO @ Sat, 15 Jan 2022 18:32:02: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:32:02: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:32:02: #1 total tags in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:32:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:32:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:32:02: #1 tags after filtering in treatment: 8817991 INFO @ Sat, 15 Jan 2022 18:32:02: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:32:02: #1 finished! INFO @ Sat, 15 Jan 2022 18:32:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:32:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:32:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:32:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:32:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917660/SRX7917660.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling