Job ID = 14520119 SRX = SRX7917659 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15373278 spots for SRR11313212/SRR11313212.sra Written 15373278 spots for SRR11313212/SRR11313212.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 15373278 reads; of these: 15373278 (100.00%) were unpaired; of these: 861444 (5.60%) aligned 0 times 12155202 (79.07%) aligned exactly 1 time 2356632 (15.33%) aligned >1 times 94.40% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4693189 / 14511834 = 0.3234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:38: 1000000 INFO @ Sat, 15 Jan 2022 18:27:43: 2000000 INFO @ Sat, 15 Jan 2022 18:27:48: 3000000 INFO @ Sat, 15 Jan 2022 18:27:53: 4000000 INFO @ Sat, 15 Jan 2022 18:27:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:03: 6000000 INFO @ Sat, 15 Jan 2022 18:28:07: 1000000 INFO @ Sat, 15 Jan 2022 18:28:08: 7000000 INFO @ Sat, 15 Jan 2022 18:28:12: 2000000 INFO @ Sat, 15 Jan 2022 18:28:13: 8000000 INFO @ Sat, 15 Jan 2022 18:28:16: 3000000 INFO @ Sat, 15 Jan 2022 18:28:18: 9000000 INFO @ Sat, 15 Jan 2022 18:28:21: 4000000 INFO @ Sat, 15 Jan 2022 18:28:22: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:28:22: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:28:22: #1 total tags in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:28:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:22: #1 tags after filtering in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:28:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:28:22: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:28:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:28:25: 5000000 INFO @ Sat, 15 Jan 2022 18:28:30: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:34: 7000000 INFO @ Sat, 15 Jan 2022 18:28:38: 1000000 INFO @ Sat, 15 Jan 2022 18:28:38: 8000000 INFO @ Sat, 15 Jan 2022 18:28:43: 9000000 INFO @ Sat, 15 Jan 2022 18:28:43: 2000000 INFO @ Sat, 15 Jan 2022 18:28:47: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:28:47: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:28:47: #1 total tags in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:28:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:47: #1 tags after filtering in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:28:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:28:47: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:28:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 18:28:48: 3000000 INFO @ Sat, 15 Jan 2022 18:28:53: 4000000 INFO @ Sat, 15 Jan 2022 18:28:58: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:29:03: 6000000 INFO @ Sat, 15 Jan 2022 18:29:08: 7000000 INFO @ Sat, 15 Jan 2022 18:29:13: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:29:18: 9000000 INFO @ Sat, 15 Jan 2022 18:29:22: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 18:29:22: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 18:29:22: #1 total tags in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:29:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:29:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:29:22: #1 tags after filtering in treatment: 9818645 INFO @ Sat, 15 Jan 2022 18:29:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 18:29:22: #1 finished! INFO @ Sat, 15 Jan 2022 18:29:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:29:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:29:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 18:29:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:29:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7917659/SRX7917659.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling