Job ID = 5791883 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,934,150 reads read : 15,934,150 reads written : 15,934,150 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:59 15934150 reads; of these: 15934150 (100.00%) were unpaired; of these: 293389 (1.84%) aligned 0 times 13477249 (84.58%) aligned exactly 1 time 2163512 (13.58%) aligned >1 times 98.16% overall alignment rate Time searching: 00:01:59 Overall time: 00:01:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5099507 / 15640761 = 0.3260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:11:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:11:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:11:34: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:11:40: 1000000 INFO @ Wed, 22 Apr 2020 11:11:45: 2000000 INFO @ Wed, 22 Apr 2020 11:11:50: 3000000 INFO @ Wed, 22 Apr 2020 11:11:55: 4000000 INFO @ Wed, 22 Apr 2020 11:12:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:12:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:12:04: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:12:04: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:12:06: 6000000 INFO @ Wed, 22 Apr 2020 11:12:12: 1000000 INFO @ Wed, 22 Apr 2020 11:12:12: 7000000 INFO @ Wed, 22 Apr 2020 11:12:19: 2000000 INFO @ Wed, 22 Apr 2020 11:12:19: 8000000 INFO @ Wed, 22 Apr 2020 11:12:25: 9000000 INFO @ Wed, 22 Apr 2020 11:12:26: 3000000 INFO @ Wed, 22 Apr 2020 11:12:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:12:33: 4000000 INFO @ Wed, 22 Apr 2020 11:12:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:12:34: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:12:34: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:12:35: #1 tag size is determined as 49 bps INFO @ Wed, 22 Apr 2020 11:12:35: #1 tag size = 49 INFO @ Wed, 22 Apr 2020 11:12:35: #1 total tags in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:12:35: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:12:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:12:35: #1 tags after filtering in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:12:35: #1 finished! INFO @ Wed, 22 Apr 2020 11:12:35: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:12:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:12:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:12:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:12:40: 5000000 INFO @ Wed, 22 Apr 2020 11:12:41: 1000000 INFO @ Wed, 22 Apr 2020 11:12:47: 6000000 INFO @ Wed, 22 Apr 2020 11:12:48: 2000000 INFO @ Wed, 22 Apr 2020 11:12:54: 3000000 INFO @ Wed, 22 Apr 2020 11:12:54: 7000000 INFO @ Wed, 22 Apr 2020 11:13:01: 4000000 INFO @ Wed, 22 Apr 2020 11:13:01: 8000000 INFO @ Wed, 22 Apr 2020 11:13:07: 5000000 INFO @ Wed, 22 Apr 2020 11:13:08: 9000000 INFO @ Wed, 22 Apr 2020 11:13:14: 6000000 INFO @ Wed, 22 Apr 2020 11:13:15: 10000000 INFO @ Wed, 22 Apr 2020 11:13:19: #1 tag size is determined as 49 bps INFO @ Wed, 22 Apr 2020 11:13:19: #1 tag size = 49 INFO @ Wed, 22 Apr 2020 11:13:19: #1 total tags in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:13:19: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:13:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:13:19: #1 tags after filtering in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:13:19: #1 finished! INFO @ Wed, 22 Apr 2020 11:13:19: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:13:20: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:13:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:13:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:13:20: 7000000 INFO @ Wed, 22 Apr 2020 11:13:26: 8000000 INFO @ Wed, 22 Apr 2020 11:13:32: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 11:13:38: 10000000 INFO @ Wed, 22 Apr 2020 11:13:41: #1 tag size is determined as 49 bps INFO @ Wed, 22 Apr 2020 11:13:41: #1 tag size = 49 INFO @ Wed, 22 Apr 2020 11:13:41: #1 total tags in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:13:41: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:13:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:13:41: #1 tags after filtering in treatment: 10541254 INFO @ Wed, 22 Apr 2020 11:13:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:13:41: #1 finished! INFO @ Wed, 22 Apr 2020 11:13:41: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:13:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:13:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:13:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:13:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897109/SRX7897109.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。