Job ID = 5791877 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,487,404 reads read : 16,487,404 reads written : 16,487,404 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:37 16487404 reads; of these: 16487404 (100.00%) were unpaired; of these: 4141036 (25.12%) aligned 0 times 10076625 (61.12%) aligned exactly 1 time 2269743 (13.77%) aligned >1 times 74.88% overall alignment rate Time searching: 00:01:37 Overall time: 00:01:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4090476 / 12346368 = 0.3313 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:10:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:10:17: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:10:17: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:10:23: 1000000 INFO @ Wed, 22 Apr 2020 11:10:28: 2000000 INFO @ Wed, 22 Apr 2020 11:10:33: 3000000 INFO @ Wed, 22 Apr 2020 11:10:38: 4000000 INFO @ Wed, 22 Apr 2020 11:10:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:10:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:10:47: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:10:47: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:10:48: 6000000 INFO @ Wed, 22 Apr 2020 11:10:54: 7000000 INFO @ Wed, 22 Apr 2020 11:10:54: 1000000 INFO @ Wed, 22 Apr 2020 11:11:00: 8000000 INFO @ Wed, 22 Apr 2020 11:11:01: 2000000 INFO @ Wed, 22 Apr 2020 11:11:02: #1 tag size is determined as 48 bps INFO @ Wed, 22 Apr 2020 11:11:02: #1 tag size = 48 INFO @ Wed, 22 Apr 2020 11:11:02: #1 total tags in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:11:02: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:11:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:11:02: #1 tags after filtering in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:11:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:11:02: #1 finished! INFO @ Wed, 22 Apr 2020 11:11:02: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:11:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:11:02: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:11:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:11:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:11:07: 3000000 INFO @ Wed, 22 Apr 2020 11:11:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 11:11:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 11:11:17: #1 read tag files... INFO @ Wed, 22 Apr 2020 11:11:17: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 11:11:20: 5000000 INFO @ Wed, 22 Apr 2020 11:11:24: 1000000 INFO @ Wed, 22 Apr 2020 11:11:26: 6000000 INFO @ Wed, 22 Apr 2020 11:11:30: 2000000 INFO @ Wed, 22 Apr 2020 11:11:32: 7000000 INFO @ Wed, 22 Apr 2020 11:11:36: 3000000 INFO @ Wed, 22 Apr 2020 11:11:38: 8000000 INFO @ Wed, 22 Apr 2020 11:11:39: #1 tag size is determined as 48 bps INFO @ Wed, 22 Apr 2020 11:11:39: #1 tag size = 48 INFO @ Wed, 22 Apr 2020 11:11:39: #1 total tags in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:11:39: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:11:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:11:39: #1 tags after filtering in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:11:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:11:39: #1 finished! INFO @ Wed, 22 Apr 2020 11:11:39: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:11:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:11:40: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:11:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:11:40: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 11:11:42: 4000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 11:11:47: 5000000 INFO @ Wed, 22 Apr 2020 11:11:53: 6000000 INFO @ Wed, 22 Apr 2020 11:11:59: 7000000 INFO @ Wed, 22 Apr 2020 11:12:04: 8000000 INFO @ Wed, 22 Apr 2020 11:12:06: #1 tag size is determined as 48 bps INFO @ Wed, 22 Apr 2020 11:12:06: #1 tag size = 48 INFO @ Wed, 22 Apr 2020 11:12:06: #1 total tags in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:12:06: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 11:12:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 11:12:06: #1 tags after filtering in treatment: 8255892 INFO @ Wed, 22 Apr 2020 11:12:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 11:12:06: #1 finished! INFO @ Wed, 22 Apr 2020 11:12:06: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 11:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 11:12:06: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 11:12:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 11:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7897106/SRX7897106.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。