Job ID = 5791853 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,859,837 reads read : 18,859,837 reads written : 18,859,837 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 18859837 reads; of these: 18859837 (100.00%) were unpaired; of these: 6493220 (34.43%) aligned 0 times 10096304 (53.53%) aligned exactly 1 time 2270313 (12.04%) aligned >1 times 65.57% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4101035 / 12366617 = 0.3316 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:53:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:53:21: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:53:21: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:53:26: 1000000 INFO @ Wed, 22 Apr 2020 10:53:31: 2000000 INFO @ Wed, 22 Apr 2020 10:53:35: 3000000 INFO @ Wed, 22 Apr 2020 10:53:40: 4000000 INFO @ Wed, 22 Apr 2020 10:53:45: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:53:50: 6000000 INFO @ Wed, 22 Apr 2020 10:53:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:53:51: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:53:51: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:53:55: 7000000 INFO @ Wed, 22 Apr 2020 10:53:56: 1000000 INFO @ Wed, 22 Apr 2020 10:54:00: 8000000 INFO @ Wed, 22 Apr 2020 10:54:01: 2000000 INFO @ Wed, 22 Apr 2020 10:54:02: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:54:02: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:54:02: #1 total tags in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:54:02: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:54:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:54:02: #1 tags after filtering in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:54:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:54:02: #1 finished! INFO @ Wed, 22 Apr 2020 10:54:02: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:54:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:54:02: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:54:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:54:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:54:06: 3000000 INFO @ Wed, 22 Apr 2020 10:54:11: 4000000 INFO @ Wed, 22 Apr 2020 10:54:16: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:54:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:54:21: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:54:21: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:54:21: 6000000 INFO @ Wed, 22 Apr 2020 10:54:26: 7000000 INFO @ Wed, 22 Apr 2020 10:54:26: 1000000 INFO @ Wed, 22 Apr 2020 10:54:31: 8000000 INFO @ Wed, 22 Apr 2020 10:54:32: 2000000 INFO @ Wed, 22 Apr 2020 10:54:33: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:54:33: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:54:33: #1 total tags in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:54:33: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:54:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:54:33: #1 tags after filtering in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:54:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:54:33: #1 finished! INFO @ Wed, 22 Apr 2020 10:54:33: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:54:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:54:33: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:54:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:54:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:54:37: 3000000 INFO @ Wed, 22 Apr 2020 10:54:42: 4000000 INFO @ Wed, 22 Apr 2020 10:54:47: 5000000 INFO @ Wed, 22 Apr 2020 10:54:52: 6000000 INFO @ Wed, 22 Apr 2020 10:54:56: 7000000 INFO @ Wed, 22 Apr 2020 10:55:01: 8000000 INFO @ Wed, 22 Apr 2020 10:55:03: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:55:03: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:55:03: #1 total tags in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:55:03: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:55:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:55:03: #1 tags after filtering in treatment: 8265582 INFO @ Wed, 22 Apr 2020 10:55:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:55:03: #1 finished! INFO @ Wed, 22 Apr 2020 10:55:03: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:55:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:55:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:55:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:55:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7887003/SRX7887003.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。