Job ID = 14520672 SRX = SRX7847371 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 1150976 spots for SRR11235553/SRR11235553.sra Written 1150976 spots for SRR11235553/SRR11235553.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:28 1150976 reads; of these: 1150976 (100.00%) were paired; of these: 211379 (18.37%) aligned concordantly 0 times 498212 (43.29%) aligned concordantly exactly 1 time 441385 (38.35%) aligned concordantly >1 times ---- 211379 pairs aligned concordantly 0 times; of these: 1129 (0.53%) aligned discordantly 1 time ---- 210250 pairs aligned 0 times concordantly or discordantly; of these: 420500 mates make up the pairs; of these: 367988 (87.51%) aligned 0 times 21577 (5.13%) aligned exactly 1 time 30935 (7.36%) aligned >1 times 84.01% overall alignment rate Time searching: 00:00:28 Overall time: 00:00:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 917915 / 936710 = 0.9799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:13: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:36:13: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:36:13: #1 total tags in treatment: 22362 INFO @ Sat, 15 Jan 2022 19:36:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:13: #1 tags after filtering in treatment: 13224 INFO @ Sat, 15 Jan 2022 19:36:13: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:36:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:13: #2 number of paired peaks: 128 WARNING @ Sat, 15 Jan 2022 19:36:13: Fewer paired peaks (128) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 128 pairs to build model! INFO @ Sat, 15 Jan 2022 19:36:13: start model_add_line... INFO @ Sat, 15 Jan 2022 19:36:13: start X-correlation... INFO @ Sat, 15 Jan 2022 19:36:13: end of X-cor INFO @ Sat, 15 Jan 2022 19:36:13: #2 finished! INFO @ Sat, 15 Jan 2022 19:36:13: #2 predicted fragment length is 78 bps INFO @ Sat, 15 Jan 2022 19:36:13: #2 alternative fragment length(s) may be 78,169,267,292,385,442,459,486,505,526,551,568 bps INFO @ Sat, 15 Jan 2022 19:36:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05_model.r INFO @ Sat, 15 Jan 2022 19:36:13: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:36:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:36:13: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:36:13: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05_peaks.xls INFO @ Sat, 15 Jan 2022 19:36:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:36:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.05_summits.bed INFO @ Sat, 15 Jan 2022 19:36:13: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (85 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:36:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:36:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:36:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:36:43: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:36:43: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:36:43: #1 total tags in treatment: 22362 INFO @ Sat, 15 Jan 2022 19:36:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:36:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:36:43: #1 tags after filtering in treatment: 13224 INFO @ Sat, 15 Jan 2022 19:36:43: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:36:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:36:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:36:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:36:43: #2 number of paired peaks: 128 WARNING @ Sat, 15 Jan 2022 19:36:43: Fewer paired peaks (128) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 128 pairs to build model! INFO @ Sat, 15 Jan 2022 19:36:43: start model_add_line... INFO @ Sat, 15 Jan 2022 19:36:43: start X-correlation... INFO @ Sat, 15 Jan 2022 19:36:43: end of X-cor INFO @ Sat, 15 Jan 2022 19:36:43: #2 finished! INFO @ Sat, 15 Jan 2022 19:36:43: #2 predicted fragment length is 78 bps INFO @ Sat, 15 Jan 2022 19:36:43: #2 alternative fragment length(s) may be 78,169,267,292,385,442,459,486,505,526,551,568 bps INFO @ Sat, 15 Jan 2022 19:36:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10_model.r INFO @ Sat, 15 Jan 2022 19:36:43: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:36:43: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:36:43: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:36:43: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10_peaks.xls INFO @ Sat, 15 Jan 2022 19:36:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:36:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.10_summits.bed INFO @ Sat, 15 Jan 2022 19:36:44: Done! pass1 - making usageList (12 chroms): 0 millis pass2 - checking and writing primary data (44 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:37:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:37:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:37:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:37:13: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:37:13: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:37:13: #1 total tags in treatment: 22362 INFO @ Sat, 15 Jan 2022 19:37:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:37:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:37:13: #1 tags after filtering in treatment: 13224 INFO @ Sat, 15 Jan 2022 19:37:13: #1 Redundant rate of treatment: 0.41 INFO @ Sat, 15 Jan 2022 19:37:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:37:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:37:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:37:13: #2 number of paired peaks: 128 WARNING @ Sat, 15 Jan 2022 19:37:13: Fewer paired peaks (128) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 128 pairs to build model! INFO @ Sat, 15 Jan 2022 19:37:13: start model_add_line... INFO @ Sat, 15 Jan 2022 19:37:13: start X-correlation... INFO @ Sat, 15 Jan 2022 19:37:13: end of X-cor INFO @ Sat, 15 Jan 2022 19:37:13: #2 finished! INFO @ Sat, 15 Jan 2022 19:37:13: #2 predicted fragment length is 78 bps INFO @ Sat, 15 Jan 2022 19:37:13: #2 alternative fragment length(s) may be 78,169,267,292,385,442,459,486,505,526,551,568 bps INFO @ Sat, 15 Jan 2022 19:37:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20_model.r INFO @ Sat, 15 Jan 2022 19:37:13: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:37:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:37:13: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:37:13: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20_peaks.xls INFO @ Sat, 15 Jan 2022 19:37:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:37:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847371/SRX7847371.20_summits.bed INFO @ Sat, 15 Jan 2022 19:37:13: Done! pass1 - making usageList (4 chroms): 0 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling