Job ID = 14520650 SRX = SRX7847367 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 20951591 spots for SRR11235549/SRR11235549.sra Written 20951591 spots for SRR11235549/SRR11235549.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:34 20951591 reads; of these: 20951591 (100.00%) were paired; of these: 2678804 (12.79%) aligned concordantly 0 times 12877497 (61.46%) aligned concordantly exactly 1 time 5395290 (25.75%) aligned concordantly >1 times ---- 2678804 pairs aligned concordantly 0 times; of these: 81924 (3.06%) aligned discordantly 1 time ---- 2596880 pairs aligned 0 times concordantly or discordantly; of these: 5193760 mates make up the pairs; of these: 4095800 (78.86%) aligned 0 times 621642 (11.97%) aligned exactly 1 time 476318 (9.17%) aligned >1 times 90.23% overall alignment rate Time searching: 00:12:34 Overall time: 00:12:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 18105471 / 18296516 = 0.9896 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:51:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:51:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:51:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:51:51: 1000000 INFO @ Sat, 15 Jan 2022 19:51:53: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:51:53: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:51:53: #1 total tags in treatment: 229423 INFO @ Sat, 15 Jan 2022 19:51:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:51:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:51:53: #1 tags after filtering in treatment: 83782 INFO @ Sat, 15 Jan 2022 19:51:53: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 19:51:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:51:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:51:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:51:53: #2 number of paired peaks: 413 WARNING @ Sat, 15 Jan 2022 19:51:53: Fewer paired peaks (413) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 413 pairs to build model! INFO @ Sat, 15 Jan 2022 19:51:53: start model_add_line... INFO @ Sat, 15 Jan 2022 19:51:53: start X-correlation... INFO @ Sat, 15 Jan 2022 19:51:53: end of X-cor INFO @ Sat, 15 Jan 2022 19:51:53: #2 finished! INFO @ Sat, 15 Jan 2022 19:51:53: #2 predicted fragment length is 169 bps INFO @ Sat, 15 Jan 2022 19:51:53: #2 alternative fragment length(s) may be 169,190 bps INFO @ Sat, 15 Jan 2022 19:51:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05_model.r INFO @ Sat, 15 Jan 2022 19:51:53: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:51:53: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:51:54: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:51:54: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05_peaks.xls INFO @ Sat, 15 Jan 2022 19:51:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:51:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.05_summits.bed INFO @ Sat, 15 Jan 2022 19:51:54: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (244 records, 4 fields): 283 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:52:20: 1000000 INFO @ Sat, 15 Jan 2022 19:52:22: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:52:22: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:52:22: #1 total tags in treatment: 229423 INFO @ Sat, 15 Jan 2022 19:52:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:22: #1 tags after filtering in treatment: 83782 INFO @ Sat, 15 Jan 2022 19:52:22: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 19:52:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:22: #2 number of paired peaks: 413 WARNING @ Sat, 15 Jan 2022 19:52:22: Fewer paired peaks (413) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 413 pairs to build model! INFO @ Sat, 15 Jan 2022 19:52:22: start model_add_line... INFO @ Sat, 15 Jan 2022 19:52:22: start X-correlation... INFO @ Sat, 15 Jan 2022 19:52:22: end of X-cor INFO @ Sat, 15 Jan 2022 19:52:22: #2 finished! INFO @ Sat, 15 Jan 2022 19:52:22: #2 predicted fragment length is 169 bps INFO @ Sat, 15 Jan 2022 19:52:22: #2 alternative fragment length(s) may be 169,190 bps INFO @ Sat, 15 Jan 2022 19:52:22: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10_model.r INFO @ Sat, 15 Jan 2022 19:52:22: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:52:22: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:52:23: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:52:23: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10_peaks.xls INFO @ Sat, 15 Jan 2022 19:52:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:52:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.10_summits.bed INFO @ Sat, 15 Jan 2022 19:52:23: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (146 records, 4 fields): 135 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:52:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:52:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:52:46: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:52:50: 1000000 INFO @ Sat, 15 Jan 2022 19:52:52: #1 tag size is determined as 36 bps INFO @ Sat, 15 Jan 2022 19:52:52: #1 tag size = 36 INFO @ Sat, 15 Jan 2022 19:52:52: #1 total tags in treatment: 229423 INFO @ Sat, 15 Jan 2022 19:52:52: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:52:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:52:52: #1 tags after filtering in treatment: 83782 INFO @ Sat, 15 Jan 2022 19:52:52: #1 Redundant rate of treatment: 0.63 INFO @ Sat, 15 Jan 2022 19:52:52: #1 finished! INFO @ Sat, 15 Jan 2022 19:52:52: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:52:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:52:52: #2 number of paired peaks: 413 WARNING @ Sat, 15 Jan 2022 19:52:52: Fewer paired peaks (413) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 413 pairs to build model! INFO @ Sat, 15 Jan 2022 19:52:52: start model_add_line... INFO @ Sat, 15 Jan 2022 19:52:52: start X-correlation... INFO @ Sat, 15 Jan 2022 19:52:52: end of X-cor INFO @ Sat, 15 Jan 2022 19:52:52: #2 finished! INFO @ Sat, 15 Jan 2022 19:52:52: #2 predicted fragment length is 169 bps INFO @ Sat, 15 Jan 2022 19:52:52: #2 alternative fragment length(s) may be 169,190 bps INFO @ Sat, 15 Jan 2022 19:52:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20_model.r INFO @ Sat, 15 Jan 2022 19:52:52: #3 Call peaks... INFO @ Sat, 15 Jan 2022 19:52:52: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Jan 2022 19:52:53: #3 Call peaks for each chromosome... INFO @ Sat, 15 Jan 2022 19:52:53: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20_peaks.xls INFO @ Sat, 15 Jan 2022 19:52:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20_peaks.narrowPeak INFO @ Sat, 15 Jan 2022 19:52:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX7847367/SRX7847367.20_summits.bed INFO @ Sat, 15 Jan 2022 19:52:53: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (84 records, 4 fields): 21 millis CompletedMACS2peakCalling