Job ID = 14521409 SRX = SRX7825261 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 4147 READS because READLEN < 1 Read 22431024 spots for SRR11212324/SRR11212324.sra Written 22431024 spots for SRR11212324/SRR11212324.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3981271 / 19000686 = 0.2095 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:34:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:34:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:34:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:34:31: 1000000 INFO @ Sat, 15 Jan 2022 21:34:38: 2000000 INFO @ Sat, 15 Jan 2022 21:34:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:34:53: 4000000 INFO @ Sat, 15 Jan 2022 21:34:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:34:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:34:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:35:01: 5000000 INFO @ Sat, 15 Jan 2022 21:35:02: 1000000 INFO @ Sat, 15 Jan 2022 21:35:10: 6000000 INFO @ Sat, 15 Jan 2022 21:35:10: 2000000 INFO @ Sat, 15 Jan 2022 21:35:18: 7000000 INFO @ Sat, 15 Jan 2022 21:35:19: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:35:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:35:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:35:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:35:27: 8000000 INFO @ Sat, 15 Jan 2022 21:35:28: 4000000 INFO @ Sat, 15 Jan 2022 21:35:35: 1000000 INFO @ Sat, 15 Jan 2022 21:35:37: 9000000 INFO @ Sat, 15 Jan 2022 21:35:38: 5000000 INFO @ Sat, 15 Jan 2022 21:35:45: 2000000 INFO @ Sat, 15 Jan 2022 21:35:47: 10000000 INFO @ Sat, 15 Jan 2022 21:35:48: 6000000 INFO @ Sat, 15 Jan 2022 21:35:57: 3000000 INFO @ Sat, 15 Jan 2022 21:35:57: 11000000 INFO @ Sat, 15 Jan 2022 21:35:58: 7000000 INFO @ Sat, 15 Jan 2022 21:36:07: 12000000 INFO @ Sat, 15 Jan 2022 21:36:08: 4000000 INFO @ Sat, 15 Jan 2022 21:36:08: 8000000 INFO @ Sat, 15 Jan 2022 21:36:17: 13000000 INFO @ Sat, 15 Jan 2022 21:36:18: 9000000 INFO @ Sat, 15 Jan 2022 21:36:19: 5000000 INFO @ Sat, 15 Jan 2022 21:36:27: 14000000 INFO @ Sat, 15 Jan 2022 21:36:28: 10000000 INFO @ Sat, 15 Jan 2022 21:36:30: 6000000 INFO @ Sat, 15 Jan 2022 21:36:38: 15000000 INFO @ Sat, 15 Jan 2022 21:36:38: 11000000 INFO @ Sat, 15 Jan 2022 21:36:41: 7000000 INFO @ Sat, 15 Jan 2022 21:36:47: 16000000 INFO @ Sat, 15 Jan 2022 21:36:48: 12000000 INFO @ Sat, 15 Jan 2022 21:36:52: 8000000 INFO @ Sat, 15 Jan 2022 21:36:57: 17000000 INFO @ Sat, 15 Jan 2022 21:36:58: 13000000 INFO @ Sat, 15 Jan 2022 21:37:03: 9000000 INFO @ Sat, 15 Jan 2022 21:37:07: 18000000 INFO @ Sat, 15 Jan 2022 21:37:07: 14000000 INFO @ Sat, 15 Jan 2022 21:37:14: 10000000 INFO @ Sat, 15 Jan 2022 21:37:16: 19000000 INFO @ Sat, 15 Jan 2022 21:37:17: 15000000 INFO @ Sat, 15 Jan 2022 21:37:25: 11000000 INFO @ Sat, 15 Jan 2022 21:37:26: 20000000 INFO @ Sat, 15 Jan 2022 21:37:27: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:37:36: 21000000 INFO @ Sat, 15 Jan 2022 21:37:36: 12000000 INFO @ Sat, 15 Jan 2022 21:37:36: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:37:46: 22000000 INFO @ Sat, 15 Jan 2022 21:37:46: 18000000 INFO @ Sat, 15 Jan 2022 21:37:47: 13000000 INFO @ Sat, 15 Jan 2022 21:37:56: 23000000 INFO @ Sat, 15 Jan 2022 21:37:56: 19000000 INFO @ Sat, 15 Jan 2022 21:37:58: 14000000 INFO @ Sat, 15 Jan 2022 21:38:06: 24000000 INFO @ Sat, 15 Jan 2022 21:38:06: 20000000 INFO @ Sat, 15 Jan 2022 21:38:09: 15000000 INFO @ Sat, 15 Jan 2022 21:38:15: 25000000 INFO @ Sat, 15 Jan 2022 21:38:16: 21000000 INFO @ Sat, 15 Jan 2022 21:38:21: 16000000 INFO @ Sat, 15 Jan 2022 21:38:25: 26000000 INFO @ Sat, 15 Jan 2022 21:38:26: 22000000 INFO @ Sat, 15 Jan 2022 21:38:31: 17000000 INFO @ Sat, 15 Jan 2022 21:38:34: 27000000 INFO @ Sat, 15 Jan 2022 21:38:35: 23000000 INFO @ Sat, 15 Jan 2022 21:38:42: 18000000 INFO @ Sat, 15 Jan 2022 21:38:44: 28000000 INFO @ Sat, 15 Jan 2022 21:38:45: 24000000 INFO @ Sat, 15 Jan 2022 21:38:53: 19000000 INFO @ Sat, 15 Jan 2022 21:38:53: 29000000 INFO @ Sat, 15 Jan 2022 21:38:55: 25000000 INFO @ Sat, 15 Jan 2022 21:39:03: 30000000 INFO @ Sat, 15 Jan 2022 21:39:04: 20000000 INFO @ Sat, 15 Jan 2022 21:39:04: 26000000 INFO @ Sat, 15 Jan 2022 21:39:05: #1 tag size is determined as 107 bps INFO @ Sat, 15 Jan 2022 21:39:05: #1 tag size = 107 INFO @ Sat, 15 Jan 2022 21:39:05: #1 total tags in treatment: 14922211 INFO @ Sat, 15 Jan 2022 21:39:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:39:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:39:06: #1 tags after filtering in treatment: 9921565 INFO @ Sat, 15 Jan 2022 21:39:06: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:39:06: #1 finished! INFO @ Sat, 15 Jan 2022 21:39:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:39:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:39:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:39:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:39:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:39:13: 27000000 INFO @ Sat, 15 Jan 2022 21:39:15: 21000000 INFO @ Sat, 15 Jan 2022 21:39:23: 28000000 INFO @ Sat, 15 Jan 2022 21:39:26: 22000000 INFO @ Sat, 15 Jan 2022 21:39:32: 29000000 INFO @ Sat, 15 Jan 2022 21:39:37: 23000000 INFO @ Sat, 15 Jan 2022 21:39:41: 30000000 INFO @ Sat, 15 Jan 2022 21:39:43: #1 tag size is determined as 107 bps INFO @ Sat, 15 Jan 2022 21:39:43: #1 tag size = 107 INFO @ Sat, 15 Jan 2022 21:39:43: #1 total tags in treatment: 14922211 INFO @ Sat, 15 Jan 2022 21:39:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:39:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:39:43: #1 tags after filtering in treatment: 9921565 INFO @ Sat, 15 Jan 2022 21:39:43: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:39:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:39:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:39:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:39:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:39:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:39:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:39:48: 24000000 INFO @ Sat, 15 Jan 2022 21:39:57: 25000000 INFO @ Sat, 15 Jan 2022 21:40:07: 26000000 INFO @ Sat, 15 Jan 2022 21:40:16: 27000000 INFO @ Sat, 15 Jan 2022 21:40:25: 28000000 INFO @ Sat, 15 Jan 2022 21:40:35: 29000000 INFO @ Sat, 15 Jan 2022 21:40:44: 30000000 INFO @ Sat, 15 Jan 2022 21:40:47: #1 tag size is determined as 107 bps INFO @ Sat, 15 Jan 2022 21:40:47: #1 tag size = 107 INFO @ Sat, 15 Jan 2022 21:40:47: #1 total tags in treatment: 14922211 INFO @ Sat, 15 Jan 2022 21:40:47: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:40:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:40:47: #1 tags after filtering in treatment: 9921565 INFO @ Sat, 15 Jan 2022 21:40:47: #1 Redundant rate of treatment: 0.34 INFO @ Sat, 15 Jan 2022 21:40:47: #1 finished! INFO @ Sat, 15 Jan 2022 21:40:47: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:40:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:40:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:40:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:40:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825261/SRX7825261.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling