Job ID = 14521408 SRX = SRX7825260 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Rejected 7191 READS because READLEN < 1 Read 22582435 spots for SRR11212323/SRR11212323.sra Written 22582435 spots for SRR11212323/SRR11212323.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error, fewer reads in file specified with -2 than in file specified with -1 terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] 1 unmatched pairs [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] 3 unmatched pairs [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4016806 / 21516916 = 0.1867 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:37:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:37:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:37:25: 1000000 INFO @ Sat, 15 Jan 2022 21:37:35: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:37:44: 3000000 INFO @ Sat, 15 Jan 2022 21:37:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:37:45: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:37:45: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:37:55: 4000000 INFO @ Sat, 15 Jan 2022 21:37:56: 1000000 INFO @ Sat, 15 Jan 2022 21:38:05: 5000000 INFO @ Sat, 15 Jan 2022 21:38:07: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:38:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:38:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:16: 6000000 INFO @ Sat, 15 Jan 2022 21:38:17: 3000000 INFO @ Sat, 15 Jan 2022 21:38:25: 1000000 INFO @ Sat, 15 Jan 2022 21:38:27: 7000000 INFO @ Sat, 15 Jan 2022 21:38:28: 4000000 INFO @ Sat, 15 Jan 2022 21:38:34: 2000000 INFO @ Sat, 15 Jan 2022 21:38:38: 5000000 INFO @ Sat, 15 Jan 2022 21:38:38: 8000000 INFO @ Sat, 15 Jan 2022 21:38:44: 3000000 INFO @ Sat, 15 Jan 2022 21:38:48: 6000000 INFO @ Sat, 15 Jan 2022 21:38:49: 9000000 INFO @ Sat, 15 Jan 2022 21:38:53: 4000000 INFO @ Sat, 15 Jan 2022 21:38:58: 7000000 INFO @ Sat, 15 Jan 2022 21:38:59: 10000000 INFO @ Sat, 15 Jan 2022 21:39:02: 5000000 INFO @ Sat, 15 Jan 2022 21:39:09: 8000000 INFO @ Sat, 15 Jan 2022 21:39:10: 11000000 INFO @ Sat, 15 Jan 2022 21:39:11: 6000000 INFO @ Sat, 15 Jan 2022 21:39:19: 9000000 INFO @ Sat, 15 Jan 2022 21:39:20: 7000000 INFO @ Sat, 15 Jan 2022 21:39:21: 12000000 INFO @ Sat, 15 Jan 2022 21:39:29: 10000000 INFO @ Sat, 15 Jan 2022 21:39:29: 8000000 INFO @ Sat, 15 Jan 2022 21:39:31: 13000000 INFO @ Sat, 15 Jan 2022 21:39:39: 9000000 INFO @ Sat, 15 Jan 2022 21:39:39: 11000000 INFO @ Sat, 15 Jan 2022 21:39:42: 14000000 INFO @ Sat, 15 Jan 2022 21:39:48: 10000000 INFO @ Sat, 15 Jan 2022 21:39:50: 12000000 INFO @ Sat, 15 Jan 2022 21:39:53: 15000000 INFO @ Sat, 15 Jan 2022 21:39:57: 11000000 INFO @ Sat, 15 Jan 2022 21:40:00: 13000000 INFO @ Sat, 15 Jan 2022 21:40:03: 16000000 INFO @ Sat, 15 Jan 2022 21:40:07: 12000000 INFO @ Sat, 15 Jan 2022 21:40:11: 14000000 INFO @ Sat, 15 Jan 2022 21:40:14: 17000000 INFO @ Sat, 15 Jan 2022 21:40:16: 13000000 INFO @ Sat, 15 Jan 2022 21:40:22: 15000000 INFO @ Sat, 15 Jan 2022 21:40:25: 18000000 INFO @ Sat, 15 Jan 2022 21:40:25: 14000000 INFO @ Sat, 15 Jan 2022 21:40:33: 16000000 INFO @ Sat, 15 Jan 2022 21:40:34: 15000000 INFO @ Sat, 15 Jan 2022 21:40:35: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:40:43: 17000000 INFO @ Sat, 15 Jan 2022 21:40:44: 16000000 INFO @ Sat, 15 Jan 2022 21:40:46: 20000000 INFO @ Sat, 15 Jan 2022 21:40:53: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:40:54: 18000000 INFO @ Sat, 15 Jan 2022 21:40:57: 21000000 INFO @ Sat, 15 Jan 2022 21:41:02: 18000000 INFO @ Sat, 15 Jan 2022 21:41:05: 19000000 INFO @ Sat, 15 Jan 2022 21:41:07: 22000000 INFO @ Sat, 15 Jan 2022 21:41:11: 19000000 INFO @ Sat, 15 Jan 2022 21:41:16: 20000000 INFO @ Sat, 15 Jan 2022 21:41:18: 23000000 INFO @ Sat, 15 Jan 2022 21:41:21: 20000000 INFO @ Sat, 15 Jan 2022 21:41:27: 21000000 INFO @ Sat, 15 Jan 2022 21:41:28: 24000000 INFO @ Sat, 15 Jan 2022 21:41:30: 21000000 INFO @ Sat, 15 Jan 2022 21:41:37: 22000000 INFO @ Sat, 15 Jan 2022 21:41:39: 25000000 INFO @ Sat, 15 Jan 2022 21:41:40: 22000000 INFO @ Sat, 15 Jan 2022 21:41:48: 23000000 INFO @ Sat, 15 Jan 2022 21:41:49: 26000000 INFO @ Sat, 15 Jan 2022 21:41:49: 23000000 INFO @ Sat, 15 Jan 2022 21:41:59: 24000000 INFO @ Sat, 15 Jan 2022 21:41:59: 27000000 INFO @ Sat, 15 Jan 2022 21:42:00: 24000000 INFO @ Sat, 15 Jan 2022 21:42:09: 25000000 INFO @ Sat, 15 Jan 2022 21:42:10: 28000000 INFO @ Sat, 15 Jan 2022 21:42:10: 25000000 INFO @ Sat, 15 Jan 2022 21:42:20: 26000000 INFO @ Sat, 15 Jan 2022 21:42:20: 29000000 INFO @ Sat, 15 Jan 2022 21:42:21: 26000000 INFO @ Sat, 15 Jan 2022 21:42:30: 27000000 INFO @ Sat, 15 Jan 2022 21:42:30: 30000000 INFO @ Sat, 15 Jan 2022 21:42:31: 27000000 INFO @ Sat, 15 Jan 2022 21:42:40: 31000000 INFO @ Sat, 15 Jan 2022 21:42:40: 28000000 INFO @ Sat, 15 Jan 2022 21:42:41: 28000000 INFO @ Sat, 15 Jan 2022 21:42:51: 32000000 INFO @ Sat, 15 Jan 2022 21:42:51: 29000000 INFO @ Sat, 15 Jan 2022 21:42:52: 29000000 INFO @ Sat, 15 Jan 2022 21:43:01: 33000000 INFO @ Sat, 15 Jan 2022 21:43:01: 30000000 INFO @ Sat, 15 Jan 2022 21:43:02: 30000000 INFO @ Sat, 15 Jan 2022 21:43:11: 31000000 INFO @ Sat, 15 Jan 2022 21:43:11: 34000000 INFO @ Sat, 15 Jan 2022 21:43:12: 31000000 INFO @ Sat, 15 Jan 2022 21:43:21: 32000000 INFO @ Sat, 15 Jan 2022 21:43:22: 35000000 INFO @ Sat, 15 Jan 2022 21:43:22: 32000000 INFO @ Sat, 15 Jan 2022 21:43:24: #1 tag size is determined as 144 bps INFO @ Sat, 15 Jan 2022 21:43:24: #1 tag size = 144 INFO @ Sat, 15 Jan 2022 21:43:24: #1 total tags in treatment: 17401213 INFO @ Sat, 15 Jan 2022 21:43:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:24: #1 tags after filtering in treatment: 11678036 INFO @ Sat, 15 Jan 2022 21:43:24: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:43:24: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:43:31: 33000000 INFO @ Sat, 15 Jan 2022 21:43:32: 33000000 INFO @ Sat, 15 Jan 2022 21:43:41: 34000000 INFO @ Sat, 15 Jan 2022 21:43:42: 34000000 INFO @ Sat, 15 Jan 2022 21:43:51: 35000000 INFO @ Sat, 15 Jan 2022 21:43:52: 35000000 INFO @ Sat, 15 Jan 2022 21:43:54: #1 tag size is determined as 144 bps INFO @ Sat, 15 Jan 2022 21:43:54: #1 tag size = 144 INFO @ Sat, 15 Jan 2022 21:43:54: #1 total tags in treatment: 17401213 INFO @ Sat, 15 Jan 2022 21:43:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:54: #1 tags after filtering in treatment: 11678036 INFO @ Sat, 15 Jan 2022 21:43:54: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:43:54: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:55: #1 tag size is determined as 144 bps INFO @ Sat, 15 Jan 2022 21:43:55: #1 tag size = 144 INFO @ Sat, 15 Jan 2022 21:43:55: #1 total tags in treatment: 17401213 INFO @ Sat, 15 Jan 2022 21:43:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:43:55: #1 tags after filtering in treatment: 11678036 INFO @ Sat, 15 Jan 2022 21:43:55: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 21:43:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7825260/SRX7825260.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling