Job ID = 14522009 SRX = SRX7756570 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 14301803 spots for SRR11119550/SRR11119550.sra Written 14301803 spots for SRR11119550/SRR11119550.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:54 14301803 reads; of these: 14301803 (100.00%) were paired; of these: 1824611 (12.76%) aligned concordantly 0 times 11273147 (78.82%) aligned concordantly exactly 1 time 1204045 (8.42%) aligned concordantly >1 times ---- 1824611 pairs aligned concordantly 0 times; of these: 1087618 (59.61%) aligned discordantly 1 time ---- 736993 pairs aligned 0 times concordantly or discordantly; of these: 1473986 mates make up the pairs; of these: 1069919 (72.59%) aligned 0 times 128724 (8.73%) aligned exactly 1 time 275343 (18.68%) aligned >1 times 96.26% overall alignment rate Time searching: 00:15:54 Overall time: 00:15:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1939351 / 13549017 = 0.1431 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:29:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:29:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:29:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:29:10: 1000000 INFO @ Sat, 15 Jan 2022 22:29:17: 2000000 INFO @ Sat, 15 Jan 2022 22:29:24: 3000000 INFO @ Sat, 15 Jan 2022 22:29:30: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:29:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:29:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:29:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:29:38: 5000000 INFO @ Sat, 15 Jan 2022 22:29:40: 1000000 INFO @ Sat, 15 Jan 2022 22:29:46: 6000000 INFO @ Sat, 15 Jan 2022 22:29:48: 2000000 INFO @ Sat, 15 Jan 2022 22:29:54: 7000000 INFO @ Sat, 15 Jan 2022 22:29:56: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:30:02: 8000000 INFO @ Sat, 15 Jan 2022 22:30:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:30:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:30:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:30:04: 4000000 INFO @ Sat, 15 Jan 2022 22:30:10: 9000000 INFO @ Sat, 15 Jan 2022 22:30:13: 5000000 INFO @ Sat, 15 Jan 2022 22:30:13: 1000000 INFO @ Sat, 15 Jan 2022 22:30:19: 10000000 INFO @ Sat, 15 Jan 2022 22:30:22: 6000000 INFO @ Sat, 15 Jan 2022 22:30:23: 2000000 INFO @ Sat, 15 Jan 2022 22:30:28: 11000000 INFO @ Sat, 15 Jan 2022 22:30:30: 7000000 INFO @ Sat, 15 Jan 2022 22:30:34: 3000000 INFO @ Sat, 15 Jan 2022 22:30:37: 12000000 INFO @ Sat, 15 Jan 2022 22:30:39: 8000000 INFO @ Sat, 15 Jan 2022 22:30:44: 4000000 INFO @ Sat, 15 Jan 2022 22:30:45: 13000000 INFO @ Sat, 15 Jan 2022 22:30:48: 9000000 INFO @ Sat, 15 Jan 2022 22:30:55: 14000000 INFO @ Sat, 15 Jan 2022 22:30:55: 5000000 INFO @ Sat, 15 Jan 2022 22:30:57: 10000000 INFO @ Sat, 15 Jan 2022 22:31:03: 15000000 INFO @ Sat, 15 Jan 2022 22:31:05: 6000000 INFO @ Sat, 15 Jan 2022 22:31:06: 11000000 INFO @ Sat, 15 Jan 2022 22:31:12: 16000000 INFO @ Sat, 15 Jan 2022 22:31:14: 12000000 INFO @ Sat, 15 Jan 2022 22:31:15: 7000000 INFO @ Sat, 15 Jan 2022 22:31:21: 17000000 INFO @ Sat, 15 Jan 2022 22:31:23: 13000000 INFO @ Sat, 15 Jan 2022 22:31:26: 8000000 INFO @ Sat, 15 Jan 2022 22:31:30: 18000000 INFO @ Sat, 15 Jan 2022 22:31:32: 14000000 INFO @ Sat, 15 Jan 2022 22:31:35: 9000000 INFO @ Sat, 15 Jan 2022 22:31:39: 19000000 INFO @ Sat, 15 Jan 2022 22:31:41: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:31:45: 10000000 INFO @ Sat, 15 Jan 2022 22:31:48: 20000000 INFO @ Sat, 15 Jan 2022 22:31:50: 16000000 INFO @ Sat, 15 Jan 2022 22:31:55: 11000000 INFO @ Sat, 15 Jan 2022 22:31:56: 21000000 INFO @ Sat, 15 Jan 2022 22:31:59: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:32:05: 12000000 INFO @ Sat, 15 Jan 2022 22:32:05: 22000000 INFO @ Sat, 15 Jan 2022 22:32:07: 18000000 INFO @ Sat, 15 Jan 2022 22:32:14: 23000000 INFO @ Sat, 15 Jan 2022 22:32:15: 13000000 INFO @ Sat, 15 Jan 2022 22:32:16: 19000000 INFO @ Sat, 15 Jan 2022 22:32:20: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:32:20: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:32:20: #1 total tags in treatment: 10663728 INFO @ Sat, 15 Jan 2022 22:32:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:32:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:32:20: #1 tags after filtering in treatment: 8103427 INFO @ Sat, 15 Jan 2022 22:32:20: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:32:20: #1 finished! INFO @ Sat, 15 Jan 2022 22:32:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:32:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:32:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:32:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:32:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:32:24: 14000000 INFO @ Sat, 15 Jan 2022 22:32:25: 20000000 INFO @ Sat, 15 Jan 2022 22:32:34: 21000000 INFO @ Sat, 15 Jan 2022 22:32:34: 15000000 INFO @ Sat, 15 Jan 2022 22:32:42: 22000000 INFO @ Sat, 15 Jan 2022 22:32:44: 16000000 INFO @ Sat, 15 Jan 2022 22:32:51: 23000000 INFO @ Sat, 15 Jan 2022 22:32:53: 17000000 INFO @ Sat, 15 Jan 2022 22:32:57: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:32:57: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:32:57: #1 total tags in treatment: 10663728 INFO @ Sat, 15 Jan 2022 22:32:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:32:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:32:57: #1 tags after filtering in treatment: 8103427 INFO @ Sat, 15 Jan 2022 22:32:57: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:32:57: #1 finished! INFO @ Sat, 15 Jan 2022 22:32:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:32:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:32:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:32:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:32:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:33:03: 18000000 INFO @ Sat, 15 Jan 2022 22:33:12: 19000000 INFO @ Sat, 15 Jan 2022 22:33:21: 20000000 INFO @ Sat, 15 Jan 2022 22:33:30: 21000000 INFO @ Sat, 15 Jan 2022 22:33:38: 22000000 INFO @ Sat, 15 Jan 2022 22:33:47: 23000000 INFO @ Sat, 15 Jan 2022 22:33:53: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:33:53: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:33:53: #1 total tags in treatment: 10663728 INFO @ Sat, 15 Jan 2022 22:33:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:33:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:33:53: #1 tags after filtering in treatment: 8103427 INFO @ Sat, 15 Jan 2022 22:33:53: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:33:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:33:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:33:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:33:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:33:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756570/SRX7756570.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling