Job ID = 14521996 SRX = SRX7756569 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18024962 spots for SRR11119551/SRR11119551.sra Written 18024962 spots for SRR11119551/SRR11119551.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:23 18024962 reads; of these: 18024962 (100.00%) were paired; of these: 2273878 (12.62%) aligned concordantly 0 times 14203664 (78.80%) aligned concordantly exactly 1 time 1547420 (8.58%) aligned concordantly >1 times ---- 2273878 pairs aligned concordantly 0 times; of these: 1239484 (54.51%) aligned discordantly 1 time ---- 1034394 pairs aligned 0 times concordantly or discordantly; of these: 2068788 mates make up the pairs; of these: 1519601 (73.45%) aligned 0 times 210612 (10.18%) aligned exactly 1 time 338575 (16.37%) aligned >1 times 95.78% overall alignment rate Time searching: 00:20:23 Overall time: 00:20:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2873361 / 16970041 = 0.1693 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:39:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:39:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:39:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:40:04: 1000000 INFO @ Sat, 15 Jan 2022 22:40:11: 2000000 INFO @ Sat, 15 Jan 2022 22:40:18: 3000000 INFO @ Sat, 15 Jan 2022 22:40:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:40:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:40:27: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:40:27: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:40:32: 5000000 INFO @ Sat, 15 Jan 2022 22:40:36: 1000000 INFO @ Sat, 15 Jan 2022 22:40:41: 6000000 INFO @ Sat, 15 Jan 2022 22:40:46: 2000000 INFO @ Sat, 15 Jan 2022 22:40:49: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:40:55: 3000000 INFO @ Sat, 15 Jan 2022 22:40:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:40:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:40:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:40:57: 8000000 INFO @ Sat, 15 Jan 2022 22:41:05: 4000000 INFO @ Sat, 15 Jan 2022 22:41:06: 1000000 INFO @ Sat, 15 Jan 2022 22:41:06: 9000000 INFO @ Sat, 15 Jan 2022 22:41:15: 5000000 INFO @ Sat, 15 Jan 2022 22:41:15: 10000000 INFO @ Sat, 15 Jan 2022 22:41:15: 2000000 INFO @ Sat, 15 Jan 2022 22:41:24: 11000000 INFO @ Sat, 15 Jan 2022 22:41:24: 3000000 INFO @ Sat, 15 Jan 2022 22:41:24: 6000000 INFO @ Sat, 15 Jan 2022 22:41:32: 12000000 INFO @ Sat, 15 Jan 2022 22:41:32: 4000000 INFO @ Sat, 15 Jan 2022 22:41:34: 7000000 INFO @ Sat, 15 Jan 2022 22:41:41: 13000000 INFO @ Sat, 15 Jan 2022 22:41:41: 5000000 INFO @ Sat, 15 Jan 2022 22:41:43: 8000000 INFO @ Sat, 15 Jan 2022 22:41:50: 14000000 INFO @ Sat, 15 Jan 2022 22:41:51: 6000000 INFO @ Sat, 15 Jan 2022 22:41:53: 9000000 INFO @ Sat, 15 Jan 2022 22:41:58: 15000000 INFO @ Sat, 15 Jan 2022 22:42:01: 7000000 INFO @ Sat, 15 Jan 2022 22:42:02: 10000000 INFO @ Sat, 15 Jan 2022 22:42:07: 16000000 INFO @ Sat, 15 Jan 2022 22:42:09: 8000000 INFO @ Sat, 15 Jan 2022 22:42:12: 11000000 INFO @ Sat, 15 Jan 2022 22:42:15: 17000000 INFO @ Sat, 15 Jan 2022 22:42:18: 9000000 INFO @ Sat, 15 Jan 2022 22:42:21: 12000000 INFO @ Sat, 15 Jan 2022 22:42:24: 18000000 INFO @ Sat, 15 Jan 2022 22:42:27: 10000000 INFO @ Sat, 15 Jan 2022 22:42:31: 13000000 INFO @ Sat, 15 Jan 2022 22:42:33: 19000000 INFO @ Sat, 15 Jan 2022 22:42:37: 11000000 INFO @ Sat, 15 Jan 2022 22:42:41: 14000000 INFO @ Sat, 15 Jan 2022 22:42:41: 20000000 INFO @ Sat, 15 Jan 2022 22:42:46: 12000000 INFO @ Sat, 15 Jan 2022 22:42:49: 21000000 INFO @ Sat, 15 Jan 2022 22:42:50: 15000000 INFO @ Sat, 15 Jan 2022 22:42:56: 13000000 INFO @ Sat, 15 Jan 2022 22:42:58: 22000000 INFO @ Sat, 15 Jan 2022 22:42:59: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:43:05: 14000000 INFO @ Sat, 15 Jan 2022 22:43:06: 23000000 INFO @ Sat, 15 Jan 2022 22:43:09: 17000000 INFO @ Sat, 15 Jan 2022 22:43:15: 24000000 INFO @ Sat, 15 Jan 2022 22:43:15: 15000000 INFO @ Sat, 15 Jan 2022 22:43:18: 18000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:43:23: 25000000 INFO @ Sat, 15 Jan 2022 22:43:24: 16000000 INFO @ Sat, 15 Jan 2022 22:43:28: 19000000 INFO @ Sat, 15 Jan 2022 22:43:31: 26000000 INFO @ Sat, 15 Jan 2022 22:43:33: 17000000 INFO @ Sat, 15 Jan 2022 22:43:37: 20000000 INFO @ Sat, 15 Jan 2022 22:43:40: 27000000 INFO @ Sat, 15 Jan 2022 22:43:43: 18000000 INFO @ Sat, 15 Jan 2022 22:43:47: 21000000 INFO @ Sat, 15 Jan 2022 22:43:48: 28000000 INFO @ Sat, 15 Jan 2022 22:43:52: 19000000 INFO @ Sat, 15 Jan 2022 22:43:55: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:43:55: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:43:55: #1 total tags in treatment: 13047095 INFO @ Sat, 15 Jan 2022 22:43:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:43:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:43:55: #1 tags after filtering in treatment: 9451130 INFO @ Sat, 15 Jan 2022 22:43:55: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:43:55: #1 finished! INFO @ Sat, 15 Jan 2022 22:43:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:43:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:43:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:43:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:43:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:43:56: 22000000 INFO @ Sat, 15 Jan 2022 22:44:01: 20000000 INFO @ Sat, 15 Jan 2022 22:44:06: 23000000 INFO @ Sat, 15 Jan 2022 22:44:11: 21000000 INFO @ Sat, 15 Jan 2022 22:44:15: 24000000 INFO @ Sat, 15 Jan 2022 22:44:20: 22000000 INFO @ Sat, 15 Jan 2022 22:44:24: 25000000 INFO @ Sat, 15 Jan 2022 22:44:29: 23000000 INFO @ Sat, 15 Jan 2022 22:44:33: 26000000 INFO @ Sat, 15 Jan 2022 22:44:38: 24000000 INFO @ Sat, 15 Jan 2022 22:44:43: 27000000 INFO @ Sat, 15 Jan 2022 22:44:48: 25000000 INFO @ Sat, 15 Jan 2022 22:44:52: 28000000 INFO @ Sat, 15 Jan 2022 22:44:57: 26000000 INFO @ Sat, 15 Jan 2022 22:44:59: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:44:59: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:44:59: #1 total tags in treatment: 13047095 INFO @ Sat, 15 Jan 2022 22:44:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:44:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:44:59: #1 tags after filtering in treatment: 9451130 INFO @ Sat, 15 Jan 2022 22:44:59: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:44:59: #1 finished! INFO @ Sat, 15 Jan 2022 22:44:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:44:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:45:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:45:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:45:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:45:06: 27000000 INFO @ Sat, 15 Jan 2022 22:45:14: 28000000 INFO @ Sat, 15 Jan 2022 22:45:21: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:45:21: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:45:21: #1 total tags in treatment: 13047095 INFO @ Sat, 15 Jan 2022 22:45:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:45:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:45:21: #1 tags after filtering in treatment: 9451130 INFO @ Sat, 15 Jan 2022 22:45:21: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:45:21: #1 finished! INFO @ Sat, 15 Jan 2022 22:45:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:45:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:45:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:45:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756569/SRX7756569.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling