Job ID = 14521995 SRX = SRX7756568 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15983236 spots for SRR11119552/SRR11119552.sra Written 15983236 spots for SRR11119552/SRR11119552.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:55 15983236 reads; of these: 15983236 (100.00%) were paired; of these: 1939201 (12.13%) aligned concordantly 0 times 12744897 (79.74%) aligned concordantly exactly 1 time 1299138 (8.13%) aligned concordantly >1 times ---- 1939201 pairs aligned concordantly 0 times; of these: 1011054 (52.14%) aligned discordantly 1 time ---- 928147 pairs aligned 0 times concordantly or discordantly; of these: 1856294 mates make up the pairs; of these: 1419953 (76.49%) aligned 0 times 175735 (9.47%) aligned exactly 1 time 260606 (14.04%) aligned >1 times 95.56% overall alignment rate Time searching: 00:17:55 Overall time: 00:17:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2401726 / 15033639 = 0.1598 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:13: 1000000 INFO @ Sat, 15 Jan 2022 22:37:20: 2000000 INFO @ Sat, 15 Jan 2022 22:37:26: 3000000 INFO @ Sat, 15 Jan 2022 22:37:33: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:37:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:37:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:37:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:37:40: 5000000 INFO @ Sat, 15 Jan 2022 22:37:43: 1000000 INFO @ Sat, 15 Jan 2022 22:37:47: 6000000 INFO @ Sat, 15 Jan 2022 22:37:50: 2000000 INFO @ Sat, 15 Jan 2022 22:37:55: 7000000 INFO @ Sat, 15 Jan 2022 22:37:57: 3000000 INFO @ Sat, 15 Jan 2022 22:38:02: 8000000 INFO @ Sat, 15 Jan 2022 22:38:04: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:38:09: 9000000 INFO @ Sat, 15 Jan 2022 22:38:10: 5000000 INFO @ Sat, 15 Jan 2022 22:38:14: 1000000 INFO @ Sat, 15 Jan 2022 22:38:16: 10000000 INFO @ Sat, 15 Jan 2022 22:38:17: 6000000 INFO @ Sat, 15 Jan 2022 22:38:22: 11000000 INFO @ Sat, 15 Jan 2022 22:38:23: 2000000 INFO @ Sat, 15 Jan 2022 22:38:24: 7000000 INFO @ Sat, 15 Jan 2022 22:38:30: 12000000 INFO @ Sat, 15 Jan 2022 22:38:31: 3000000 INFO @ Sat, 15 Jan 2022 22:38:31: 8000000 INFO @ Sat, 15 Jan 2022 22:38:37: 13000000 INFO @ Sat, 15 Jan 2022 22:38:38: 9000000 INFO @ Sat, 15 Jan 2022 22:38:39: 4000000 INFO @ Sat, 15 Jan 2022 22:38:45: 14000000 INFO @ Sat, 15 Jan 2022 22:38:45: 10000000 INFO @ Sat, 15 Jan 2022 22:38:48: 5000000 INFO @ Sat, 15 Jan 2022 22:38:52: 15000000 INFO @ Sat, 15 Jan 2022 22:38:52: 11000000 INFO @ Sat, 15 Jan 2022 22:38:56: 6000000 INFO @ Sat, 15 Jan 2022 22:38:59: 16000000 INFO @ Sat, 15 Jan 2022 22:39:00: 12000000 INFO @ Sat, 15 Jan 2022 22:39:04: 7000000 INFO @ Sat, 15 Jan 2022 22:39:06: 17000000 INFO @ Sat, 15 Jan 2022 22:39:07: 13000000 INFO @ Sat, 15 Jan 2022 22:39:13: 8000000 INFO @ Sat, 15 Jan 2022 22:39:13: 18000000 INFO @ Sat, 15 Jan 2022 22:39:14: 14000000 INFO @ Sat, 15 Jan 2022 22:39:20: 19000000 INFO @ Sat, 15 Jan 2022 22:39:21: 15000000 INFO @ Sat, 15 Jan 2022 22:39:21: 9000000 INFO @ Sat, 15 Jan 2022 22:39:28: 16000000 INFO @ Sat, 15 Jan 2022 22:39:28: 20000000 INFO @ Sat, 15 Jan 2022 22:39:30: 10000000 INFO @ Sat, 15 Jan 2022 22:39:34: 17000000 INFO @ Sat, 15 Jan 2022 22:39:35: 21000000 INFO @ Sat, 15 Jan 2022 22:39:38: 11000000 INFO @ Sat, 15 Jan 2022 22:39:41: 18000000 INFO @ Sat, 15 Jan 2022 22:39:42: 22000000 INFO @ Sat, 15 Jan 2022 22:39:46: 12000000 INFO @ Sat, 15 Jan 2022 22:39:48: 19000000 INFO @ Sat, 15 Jan 2022 22:39:48: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:39:55: 13000000 INFO @ Sat, 15 Jan 2022 22:39:55: 20000000 INFO @ Sat, 15 Jan 2022 22:39:55: 24000000 INFO @ Sat, 15 Jan 2022 22:40:02: 21000000 INFO @ Sat, 15 Jan 2022 22:40:02: 25000000 INFO @ Sat, 15 Jan 2022 22:40:03: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:40:07: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:40:07: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:40:07: #1 total tags in treatment: 11770366 INFO @ Sat, 15 Jan 2022 22:40:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:08: #1 tags after filtering in treatment: 8860243 INFO @ Sat, 15 Jan 2022 22:40:08: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:40:08: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Sat, 15 Jan 2022 22:40:08: 22000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:40:11: 15000000 INFO @ Sat, 15 Jan 2022 22:40:15: 23000000 INFO @ Sat, 15 Jan 2022 22:40:19: 16000000 INFO @ Sat, 15 Jan 2022 22:40:22: 24000000 INFO @ Sat, 15 Jan 2022 22:40:27: 17000000 INFO @ Sat, 15 Jan 2022 22:40:29: 25000000 INFO @ Sat, 15 Jan 2022 22:40:34: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:40:34: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:40:34: #1 total tags in treatment: 11770366 INFO @ Sat, 15 Jan 2022 22:40:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:40:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:40:34: #1 tags after filtering in treatment: 8860243 INFO @ Sat, 15 Jan 2022 22:40:34: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:40:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:40:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:40:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:40:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:40:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:40:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:40:35: 18000000 INFO @ Sat, 15 Jan 2022 22:40:43: 19000000 INFO @ Sat, 15 Jan 2022 22:40:50: 20000000 INFO @ Sat, 15 Jan 2022 22:40:58: 21000000 INFO @ Sat, 15 Jan 2022 22:41:05: 22000000 INFO @ Sat, 15 Jan 2022 22:41:13: 23000000 INFO @ Sat, 15 Jan 2022 22:41:21: 24000000 INFO @ Sat, 15 Jan 2022 22:41:29: 25000000 INFO @ Sat, 15 Jan 2022 22:41:35: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:41:35: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:41:35: #1 total tags in treatment: 11770366 INFO @ Sat, 15 Jan 2022 22:41:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:41:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:41:35: #1 tags after filtering in treatment: 8860243 INFO @ Sat, 15 Jan 2022 22:41:35: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:41:35: #1 finished! INFO @ Sat, 15 Jan 2022 22:41:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:41:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:41:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:41:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:41:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756568/SRX7756568.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling