Job ID = 14521994 SRX = SRX7756567 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17180452 spots for SRR11119553/SRR11119553.sra Written 17180452 spots for SRR11119553/SRR11119553.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:39 17180452 reads; of these: 17180452 (100.00%) were paired; of these: 2740794 (15.95%) aligned concordantly 0 times 13095826 (76.23%) aligned concordantly exactly 1 time 1343832 (7.82%) aligned concordantly >1 times ---- 2740794 pairs aligned concordantly 0 times; of these: 1353233 (49.37%) aligned discordantly 1 time ---- 1387561 pairs aligned 0 times concordantly or discordantly; of these: 2775122 mates make up the pairs; of these: 1926045 (69.40%) aligned 0 times 487191 (17.56%) aligned exactly 1 time 361886 (13.04%) aligned >1 times 94.39% overall alignment rate Time searching: 00:28:39 Overall time: 00:28:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2277004 / 15770916 = 0.1444 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:46:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:46:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:46:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:46:36: 1000000 INFO @ Sat, 15 Jan 2022 22:46:46: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:46:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:46:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:46:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:46:57: 3000000 INFO @ Sat, 15 Jan 2022 22:47:08: 1000000 INFO @ Sat, 15 Jan 2022 22:47:08: 4000000 INFO @ Sat, 15 Jan 2022 22:47:19: 5000000 INFO @ Sat, 15 Jan 2022 22:47:19: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:47:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:47:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:47:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:47:30: 6000000 INFO @ Sat, 15 Jan 2022 22:47:31: 3000000 INFO @ Sat, 15 Jan 2022 22:47:38: 1000000 INFO @ Sat, 15 Jan 2022 22:47:41: 7000000 INFO @ Sat, 15 Jan 2022 22:47:44: 4000000 INFO @ Sat, 15 Jan 2022 22:47:52: 2000000 INFO @ Sat, 15 Jan 2022 22:47:52: 8000000 INFO @ Sat, 15 Jan 2022 22:47:57: 5000000 INFO @ Sat, 15 Jan 2022 22:48:04: 9000000 INFO @ Sat, 15 Jan 2022 22:48:05: 3000000 INFO @ Sat, 15 Jan 2022 22:48:10: 6000000 INFO @ Sat, 15 Jan 2022 22:48:15: 10000000 INFO @ Sat, 15 Jan 2022 22:48:19: 4000000 INFO @ Sat, 15 Jan 2022 22:48:23: 7000000 INFO @ Sat, 15 Jan 2022 22:48:26: 11000000 INFO @ Sat, 15 Jan 2022 22:48:32: 5000000 INFO @ Sat, 15 Jan 2022 22:48:36: 8000000 INFO @ Sat, 15 Jan 2022 22:48:38: 12000000 INFO @ Sat, 15 Jan 2022 22:48:45: 6000000 INFO @ Sat, 15 Jan 2022 22:48:49: 13000000 INFO @ Sat, 15 Jan 2022 22:48:49: 9000000 INFO @ Sat, 15 Jan 2022 22:48:57: 7000000 INFO @ Sat, 15 Jan 2022 22:49:00: 14000000 INFO @ Sat, 15 Jan 2022 22:49:02: 10000000 INFO @ Sat, 15 Jan 2022 22:49:10: 8000000 INFO @ Sat, 15 Jan 2022 22:49:11: 15000000 INFO @ Sat, 15 Jan 2022 22:49:15: 11000000 INFO @ Sat, 15 Jan 2022 22:49:22: 16000000 INFO @ Sat, 15 Jan 2022 22:49:23: 9000000 INFO @ Sat, 15 Jan 2022 22:49:28: 12000000 INFO @ Sat, 15 Jan 2022 22:49:33: 17000000 INFO @ Sat, 15 Jan 2022 22:49:35: 10000000 INFO @ Sat, 15 Jan 2022 22:49:41: 13000000 INFO @ Sat, 15 Jan 2022 22:49:44: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:49:49: 11000000 INFO @ Sat, 15 Jan 2022 22:49:54: 14000000 INFO @ Sat, 15 Jan 2022 22:49:55: 19000000 INFO @ Sat, 15 Jan 2022 22:50:02: 12000000 INFO @ Sat, 15 Jan 2022 22:50:06: 15000000 INFO @ Sat, 15 Jan 2022 22:50:06: 20000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:50:14: 13000000 INFO @ Sat, 15 Jan 2022 22:50:17: 21000000 INFO @ Sat, 15 Jan 2022 22:50:19: 16000000 INFO @ Sat, 15 Jan 2022 22:50:27: 14000000 INFO @ Sat, 15 Jan 2022 22:50:29: 22000000 INFO @ Sat, 15 Jan 2022 22:50:32: 17000000 INFO @ Sat, 15 Jan 2022 22:50:40: 23000000 INFO @ Sat, 15 Jan 2022 22:50:40: 15000000 INFO @ Sat, 15 Jan 2022 22:50:45: 18000000 INFO @ Sat, 15 Jan 2022 22:50:51: 24000000 INFO @ Sat, 15 Jan 2022 22:50:53: 16000000 INFO @ Sat, 15 Jan 2022 22:50:57: 19000000 INFO @ Sat, 15 Jan 2022 22:51:02: 25000000 INFO @ Sat, 15 Jan 2022 22:51:06: 17000000 INFO @ Sat, 15 Jan 2022 22:51:10: 20000000 INFO @ Sat, 15 Jan 2022 22:51:13: 26000000 INFO @ Sat, 15 Jan 2022 22:51:18: 18000000 INFO @ Sat, 15 Jan 2022 22:51:23: 21000000 INFO @ Sat, 15 Jan 2022 22:51:24: 27000000 INFO @ Sat, 15 Jan 2022 22:51:31: 19000000 INFO @ Sat, 15 Jan 2022 22:51:34: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:51:34: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:51:34: #1 total tags in treatment: 12322886 INFO @ Sat, 15 Jan 2022 22:51:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:51:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:51:34: #1 tags after filtering in treatment: 9116032 INFO @ Sat, 15 Jan 2022 22:51:34: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:51:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:51:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:51:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:51:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:51:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:51:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:51:36: 22000000 INFO @ Sat, 15 Jan 2022 22:51:44: 20000000 INFO @ Sat, 15 Jan 2022 22:51:48: 23000000 INFO @ Sat, 15 Jan 2022 22:51:57: 21000000 INFO @ Sat, 15 Jan 2022 22:52:00: 24000000 INFO @ Sat, 15 Jan 2022 22:52:10: 22000000 INFO @ Sat, 15 Jan 2022 22:52:13: 25000000 INFO @ Sat, 15 Jan 2022 22:52:22: 23000000 INFO @ Sat, 15 Jan 2022 22:52:25: 26000000 INFO @ Sat, 15 Jan 2022 22:52:35: 24000000 INFO @ Sat, 15 Jan 2022 22:52:38: 27000000 INFO @ Sat, 15 Jan 2022 22:52:47: 25000000 INFO @ Sat, 15 Jan 2022 22:52:49: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:52:49: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:52:49: #1 total tags in treatment: 12322886 INFO @ Sat, 15 Jan 2022 22:52:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:52:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:52:50: #1 tags after filtering in treatment: 9116032 INFO @ Sat, 15 Jan 2022 22:52:50: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:52:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:52:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:52:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:52:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:52:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:52:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:52:59: 26000000 INFO @ Sat, 15 Jan 2022 22:53:10: 27000000 INFO @ Sat, 15 Jan 2022 22:53:20: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:53:20: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:53:20: #1 total tags in treatment: 12322886 INFO @ Sat, 15 Jan 2022 22:53:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:53:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:53:20: #1 tags after filtering in treatment: 9116032 INFO @ Sat, 15 Jan 2022 22:53:20: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 22:53:20: #1 finished! INFO @ Sat, 15 Jan 2022 22:53:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:53:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:53:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:53:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:53:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756567/SRX7756567.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling