Job ID = 14521967 SRX = SRX7756556 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15840561 spots for SRR11119564/SRR11119564.sra Written 15840561 spots for SRR11119564/SRR11119564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:41 15840561 reads; of these: 15840561 (100.00%) were paired; of these: 2000603 (12.63%) aligned concordantly 0 times 12511986 (78.99%) aligned concordantly exactly 1 time 1327972 (8.38%) aligned concordantly >1 times ---- 2000603 pairs aligned concordantly 0 times; of these: 979205 (48.95%) aligned discordantly 1 time ---- 1021398 pairs aligned 0 times concordantly or discordantly; of these: 2042796 mates make up the pairs; of these: 1557076 (76.22%) aligned 0 times 223744 (10.95%) aligned exactly 1 time 261976 (12.82%) aligned >1 times 95.09% overall alignment rate Time searching: 00:16:41 Overall time: 00:16:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2289313 / 14800673 = 0.1547 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:26:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:26:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:26:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:26:57: 1000000 INFO @ Sat, 15 Jan 2022 22:27:06: 2000000 INFO @ Sat, 15 Jan 2022 22:27:15: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:27:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:27:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:27:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:27:25: 4000000 INFO @ Sat, 15 Jan 2022 22:27:29: 1000000 INFO @ Sat, 15 Jan 2022 22:27:35: 5000000 INFO @ Sat, 15 Jan 2022 22:27:39: 2000000 INFO @ Sat, 15 Jan 2022 22:27:44: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:27:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:27:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:27:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:27:49: 3000000 INFO @ Sat, 15 Jan 2022 22:27:54: 7000000 INFO @ Sat, 15 Jan 2022 22:28:00: 1000000 INFO @ Sat, 15 Jan 2022 22:28:00: 4000000 INFO @ Sat, 15 Jan 2022 22:28:04: 8000000 INFO @ Sat, 15 Jan 2022 22:28:11: 5000000 INFO @ Sat, 15 Jan 2022 22:28:11: 2000000 INFO @ Sat, 15 Jan 2022 22:28:14: 9000000 INFO @ Sat, 15 Jan 2022 22:28:22: 6000000 INFO @ Sat, 15 Jan 2022 22:28:22: 3000000 INFO @ Sat, 15 Jan 2022 22:28:24: 10000000 INFO @ Sat, 15 Jan 2022 22:28:32: 7000000 INFO @ Sat, 15 Jan 2022 22:28:34: 4000000 INFO @ Sat, 15 Jan 2022 22:28:34: 11000000 INFO @ Sat, 15 Jan 2022 22:28:43: 8000000 INFO @ Sat, 15 Jan 2022 22:28:43: 12000000 INFO @ Sat, 15 Jan 2022 22:28:45: 5000000 INFO @ Sat, 15 Jan 2022 22:28:53: 9000000 INFO @ Sat, 15 Jan 2022 22:28:53: 13000000 INFO @ Sat, 15 Jan 2022 22:28:56: 6000000 INFO @ Sat, 15 Jan 2022 22:29:03: 14000000 INFO @ Sat, 15 Jan 2022 22:29:03: 10000000 INFO @ Sat, 15 Jan 2022 22:29:07: 7000000 INFO @ Sat, 15 Jan 2022 22:29:13: 15000000 INFO @ Sat, 15 Jan 2022 22:29:14: 11000000 INFO @ Sat, 15 Jan 2022 22:29:18: 8000000 INFO @ Sat, 15 Jan 2022 22:29:23: 16000000 INFO @ Sat, 15 Jan 2022 22:29:24: 12000000 INFO @ Sat, 15 Jan 2022 22:29:28: 9000000 INFO @ Sat, 15 Jan 2022 22:29:33: 17000000 INFO @ Sat, 15 Jan 2022 22:29:34: 13000000 INFO @ Sat, 15 Jan 2022 22:29:38: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:29:43: 18000000 INFO @ Sat, 15 Jan 2022 22:29:44: 14000000 INFO @ Sat, 15 Jan 2022 22:29:49: 11000000 INFO @ Sat, 15 Jan 2022 22:29:53: 19000000 INFO @ Sat, 15 Jan 2022 22:29:54: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:29:59: 12000000 INFO @ Sat, 15 Jan 2022 22:30:03: 20000000 INFO @ Sat, 15 Jan 2022 22:30:04: 16000000 INFO @ Sat, 15 Jan 2022 22:30:09: 13000000 INFO @ Sat, 15 Jan 2022 22:30:12: 21000000 INFO @ Sat, 15 Jan 2022 22:30:14: 17000000 INFO @ Sat, 15 Jan 2022 22:30:20: 14000000 INFO @ Sat, 15 Jan 2022 22:30:22: 22000000 INFO @ Sat, 15 Jan 2022 22:30:24: 18000000 INFO @ Sat, 15 Jan 2022 22:30:31: 15000000 INFO @ Sat, 15 Jan 2022 22:30:32: 23000000 INFO @ Sat, 15 Jan 2022 22:30:34: 19000000 INFO @ Sat, 15 Jan 2022 22:30:42: 16000000 INFO @ Sat, 15 Jan 2022 22:30:42: 24000000 INFO @ Sat, 15 Jan 2022 22:30:45: 20000000 INFO @ Sat, 15 Jan 2022 22:30:52: 25000000 INFO @ Sat, 15 Jan 2022 22:30:53: 17000000 INFO @ Sat, 15 Jan 2022 22:30:55: 21000000 INFO @ Sat, 15 Jan 2022 22:30:58: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:30:58: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:30:58: #1 total tags in treatment: 11674633 INFO @ Sat, 15 Jan 2022 22:30:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:30:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:30:58: #1 tags after filtering in treatment: 8711253 INFO @ Sat, 15 Jan 2022 22:30:58: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:30:58: #1 finished! INFO @ Sat, 15 Jan 2022 22:30:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:30:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:30:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:30:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:30:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:31:02: 18000000 INFO @ Sat, 15 Jan 2022 22:31:04: 22000000 INFO @ Sat, 15 Jan 2022 22:31:12: 19000000 INFO @ Sat, 15 Jan 2022 22:31:14: 23000000 INFO @ Sat, 15 Jan 2022 22:31:22: 20000000 INFO @ Sat, 15 Jan 2022 22:31:24: 24000000 INFO @ Sat, 15 Jan 2022 22:31:32: 21000000 INFO @ Sat, 15 Jan 2022 22:31:33: 25000000 INFO @ Sat, 15 Jan 2022 22:31:38: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:31:38: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:31:38: #1 total tags in treatment: 11674633 INFO @ Sat, 15 Jan 2022 22:31:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:31:39: #1 tags after filtering in treatment: 8711253 INFO @ Sat, 15 Jan 2022 22:31:39: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:31:39: #1 finished! INFO @ Sat, 15 Jan 2022 22:31:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:31:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:31:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:31:41: 22000000 INFO @ Sat, 15 Jan 2022 22:31:50: 23000000 INFO @ Sat, 15 Jan 2022 22:31:59: 24000000 INFO @ Sat, 15 Jan 2022 22:32:08: 25000000 INFO @ Sat, 15 Jan 2022 22:32:12: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:32:12: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:32:12: #1 total tags in treatment: 11674633 INFO @ Sat, 15 Jan 2022 22:32:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:32:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:32:13: #1 tags after filtering in treatment: 8711253 INFO @ Sat, 15 Jan 2022 22:32:13: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:32:13: #1 finished! INFO @ Sat, 15 Jan 2022 22:32:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:32:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:32:13: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:32:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:32:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756556/SRX7756556.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling