Job ID = 14521966 SRX = SRX7756555 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15628596 spots for SRR11119565/SRR11119565.sra Written 15628596 spots for SRR11119565/SRR11119565.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:59 15628596 reads; of these: 15628596 (100.00%) were paired; of these: 1553841 (9.94%) aligned concordantly 0 times 12513660 (80.07%) aligned concordantly exactly 1 time 1561095 (9.99%) aligned concordantly >1 times ---- 1553841 pairs aligned concordantly 0 times; of these: 735268 (47.32%) aligned discordantly 1 time ---- 818573 pairs aligned 0 times concordantly or discordantly; of these: 1637146 mates make up the pairs; of these: 1239484 (75.71%) aligned 0 times 141087 (8.62%) aligned exactly 1 time 256575 (15.67%) aligned >1 times 96.03% overall alignment rate Time searching: 00:24:59 Overall time: 00:24:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2175267 / 14785922 = 0.1471 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:38:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:38:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:38:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:39:00: 1000000 INFO @ Sat, 15 Jan 2022 22:39:10: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:39:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:39:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:39:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:39:20: 3000000 INFO @ Sat, 15 Jan 2022 22:39:29: 1000000 INFO @ Sat, 15 Jan 2022 22:39:30: 4000000 INFO @ Sat, 15 Jan 2022 22:39:38: 2000000 INFO @ Sat, 15 Jan 2022 22:39:41: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:39:48: 3000000 INFO @ Sat, 15 Jan 2022 22:39:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:39:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:39:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:39:51: 6000000 INFO @ Sat, 15 Jan 2022 22:39:58: 4000000 INFO @ Sat, 15 Jan 2022 22:40:00: 1000000 INFO @ Sat, 15 Jan 2022 22:40:02: 7000000 INFO @ Sat, 15 Jan 2022 22:40:09: 5000000 INFO @ Sat, 15 Jan 2022 22:40:10: 2000000 INFO @ Sat, 15 Jan 2022 22:40:13: 8000000 INFO @ Sat, 15 Jan 2022 22:40:19: 6000000 INFO @ Sat, 15 Jan 2022 22:40:20: 3000000 INFO @ Sat, 15 Jan 2022 22:40:24: 9000000 INFO @ Sat, 15 Jan 2022 22:40:30: 7000000 INFO @ Sat, 15 Jan 2022 22:40:30: 4000000 INFO @ Sat, 15 Jan 2022 22:40:35: 10000000 INFO @ Sat, 15 Jan 2022 22:40:40: 5000000 INFO @ Sat, 15 Jan 2022 22:40:41: 8000000 INFO @ Sat, 15 Jan 2022 22:40:46: 11000000 INFO @ Sat, 15 Jan 2022 22:40:50: 6000000 INFO @ Sat, 15 Jan 2022 22:40:52: 9000000 INFO @ Sat, 15 Jan 2022 22:40:56: 12000000 INFO @ Sat, 15 Jan 2022 22:40:59: 7000000 INFO @ Sat, 15 Jan 2022 22:41:03: 10000000 INFO @ Sat, 15 Jan 2022 22:41:07: 13000000 INFO @ Sat, 15 Jan 2022 22:41:08: 8000000 INFO @ Sat, 15 Jan 2022 22:41:13: 11000000 INFO @ Sat, 15 Jan 2022 22:41:17: 9000000 INFO @ Sat, 15 Jan 2022 22:41:18: 14000000 INFO @ Sat, 15 Jan 2022 22:41:24: 12000000 INFO @ Sat, 15 Jan 2022 22:41:27: 10000000 INFO @ Sat, 15 Jan 2022 22:41:28: 15000000 INFO @ Sat, 15 Jan 2022 22:41:34: 13000000 INFO @ Sat, 15 Jan 2022 22:41:36: 11000000 INFO @ Sat, 15 Jan 2022 22:41:39: 16000000 INFO @ Sat, 15 Jan 2022 22:41:45: 14000000 INFO @ Sat, 15 Jan 2022 22:41:45: 12000000 INFO @ Sat, 15 Jan 2022 22:41:49: 17000000 INFO @ Sat, 15 Jan 2022 22:41:55: 13000000 INFO @ Sat, 15 Jan 2022 22:41:55: 15000000 INFO @ Sat, 15 Jan 2022 22:42:00: 18000000 INFO @ Sat, 15 Jan 2022 22:42:04: 14000000 INFO @ Sat, 15 Jan 2022 22:42:05: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:42:10: 19000000 INFO @ Sat, 15 Jan 2022 22:42:13: 15000000 INFO @ Sat, 15 Jan 2022 22:42:16: 17000000 INFO @ Sat, 15 Jan 2022 22:42:20: 20000000 INFO @ Sat, 15 Jan 2022 22:42:22: 16000000 INFO @ Sat, 15 Jan 2022 22:42:26: 18000000 INFO @ Sat, 15 Jan 2022 22:42:31: 21000000 INFO @ Sat, 15 Jan 2022 22:42:31: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:42:37: 19000000 INFO @ Sat, 15 Jan 2022 22:42:40: 18000000 INFO @ Sat, 15 Jan 2022 22:42:41: 22000000 INFO @ Sat, 15 Jan 2022 22:42:47: 20000000 INFO @ Sat, 15 Jan 2022 22:42:49: 19000000 INFO @ Sat, 15 Jan 2022 22:42:51: 23000000 INFO @ Sat, 15 Jan 2022 22:42:57: 21000000 INFO @ Sat, 15 Jan 2022 22:42:58: 20000000 INFO @ Sat, 15 Jan 2022 22:43:02: 24000000 INFO @ Sat, 15 Jan 2022 22:43:07: 21000000 INFO @ Sat, 15 Jan 2022 22:43:08: 22000000 INFO @ Sat, 15 Jan 2022 22:43:12: 25000000 INFO @ Sat, 15 Jan 2022 22:43:16: 22000000 INFO @ Sat, 15 Jan 2022 22:43:18: 23000000 INFO @ Sat, 15 Jan 2022 22:43:19: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:43:19: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:43:19: #1 total tags in treatment: 11986515 INFO @ Sat, 15 Jan 2022 22:43:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:43:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:43:19: #1 tags after filtering in treatment: 8737861 INFO @ Sat, 15 Jan 2022 22:43:19: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:43:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:43:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:43:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:43:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:43:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:43:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:43:25: 23000000 INFO @ Sat, 15 Jan 2022 22:43:29: 24000000 INFO @ Sat, 15 Jan 2022 22:43:34: 24000000 INFO @ Sat, 15 Jan 2022 22:43:39: 25000000 INFO @ Sat, 15 Jan 2022 22:43:43: 25000000 INFO @ Sat, 15 Jan 2022 22:43:46: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:43:46: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:43:46: #1 total tags in treatment: 11986515 INFO @ Sat, 15 Jan 2022 22:43:46: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:43:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:43:46: #1 tags after filtering in treatment: 8737861 INFO @ Sat, 15 Jan 2022 22:43:46: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:43:46: #1 finished! INFO @ Sat, 15 Jan 2022 22:43:46: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:43:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:43:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:43:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:43:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:43:49: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 22:43:49: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 22:43:49: #1 total tags in treatment: 11986515 INFO @ Sat, 15 Jan 2022 22:43:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:43:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:43:50: #1 tags after filtering in treatment: 8737861 INFO @ Sat, 15 Jan 2022 22:43:50: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 22:43:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:43:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:43:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:43:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:43:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:43:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7756555/SRX7756555.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling