Job ID = 5791724 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,134,328 reads read : 25,134,328 reads written : 25,134,328 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 25134328 reads; of these: 25134328 (100.00%) were unpaired; of these: 1241470 (4.94%) aligned 0 times 15983383 (63.59%) aligned exactly 1 time 7909475 (31.47%) aligned >1 times 95.06% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12842965 / 23892858 = 0.5375 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:55:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:55:38: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:55:38: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:55:44: 1000000 INFO @ Wed, 22 Apr 2020 10:55:51: 2000000 INFO @ Wed, 22 Apr 2020 10:55:57: 3000000 INFO @ Wed, 22 Apr 2020 10:56:04: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:56:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:56:09: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:56:09: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:56:10: 5000000 INFO @ Wed, 22 Apr 2020 10:56:15: 1000000 INFO @ Wed, 22 Apr 2020 10:56:17: 6000000 INFO @ Wed, 22 Apr 2020 10:56:21: 2000000 INFO @ Wed, 22 Apr 2020 10:56:24: 7000000 INFO @ Wed, 22 Apr 2020 10:56:27: 3000000 INFO @ Wed, 22 Apr 2020 10:56:31: 8000000 INFO @ Wed, 22 Apr 2020 10:56:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:56:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:56:36: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:56:36: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:56:37: 9000000 INFO @ Wed, 22 Apr 2020 10:56:40: 5000000 INFO @ Wed, 22 Apr 2020 10:56:43: 1000000 INFO @ Wed, 22 Apr 2020 10:56:44: 10000000 INFO @ Wed, 22 Apr 2020 10:56:46: 6000000 INFO @ Wed, 22 Apr 2020 10:56:49: 2000000 INFO @ Wed, 22 Apr 2020 10:56:51: 11000000 INFO @ Wed, 22 Apr 2020 10:56:51: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:56:51: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:56:51: #1 total tags in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:56:51: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:56:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:56:52: #1 tags after filtering in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:56:52: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:56:52: #1 finished! INFO @ Wed, 22 Apr 2020 10:56:52: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:56:52: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:56:52: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:56:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:56:52: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 10:56:52: 7000000 INFO @ Wed, 22 Apr 2020 10:56:55: 3000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:56:58: 8000000 INFO @ Wed, 22 Apr 2020 10:57:01: 4000000 INFO @ Wed, 22 Apr 2020 10:57:05: 9000000 INFO @ Wed, 22 Apr 2020 10:57:07: 5000000 INFO @ Wed, 22 Apr 2020 10:57:11: 10000000 INFO @ Wed, 22 Apr 2020 10:57:13: 6000000 INFO @ Wed, 22 Apr 2020 10:57:17: 11000000 INFO @ Wed, 22 Apr 2020 10:57:17: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:57:17: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:57:17: #1 total tags in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:57:17: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:57:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:57:17: #1 tags after filtering in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:57:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:57:17: #1 finished! INFO @ Wed, 22 Apr 2020 10:57:17: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:57:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:57:18: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:57:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:57:18: Process for pairing-model is terminated! INFO @ Wed, 22 Apr 2020 10:57:19: 7000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 10:57:25: 8000000 INFO @ Wed, 22 Apr 2020 10:57:30: 9000000 INFO @ Wed, 22 Apr 2020 10:57:36: 10000000 INFO @ Wed, 22 Apr 2020 10:57:41: 11000000 INFO @ Wed, 22 Apr 2020 10:57:42: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 10:57:42: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 10:57:42: #1 total tags in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:57:42: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:57:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:57:42: #1 tags after filtering in treatment: 11049893 INFO @ Wed, 22 Apr 2020 10:57:42: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:57:42: #1 finished! INFO @ Wed, 22 Apr 2020 10:57:42: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:57:42: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Apr 2020 10:57:42: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 10:57:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:57:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7700982/SRX7700982.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。