Job ID = 5791704 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,540,602 reads read : 7,540,602 reads written : 7,540,602 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:43 7540602 reads; of these: 7540602 (100.00%) were unpaired; of these: 4726466 (62.68%) aligned 0 times 2235973 (29.65%) aligned exactly 1 time 578163 (7.67%) aligned >1 times 37.32% overall alignment rate Time searching: 00:00:43 Overall time: 00:00:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 610520 / 2814136 = 0.2169 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:35:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:35:57: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:35:57: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:36:03: 1000000 INFO @ Wed, 22 Apr 2020 10:36:08: 2000000 INFO @ Wed, 22 Apr 2020 10:36:09: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:36:09: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:36:09: #1 total tags in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:36:09: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:36:09: #1 tags after filtering in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:36:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:36:09: #1 finished! INFO @ Wed, 22 Apr 2020 10:36:09: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:36:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:36:09: #2 number of paired peaks: 80 WARNING @ Wed, 22 Apr 2020 10:36:09: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:36:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:36:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:27: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:27: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:36:33: 1000000 INFO @ Wed, 22 Apr 2020 10:36:38: 2000000 INFO @ Wed, 22 Apr 2020 10:36:40: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:36:40: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:36:40: #1 total tags in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:36:40: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:36:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:36:40: #1 tags after filtering in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:36:40: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:36:40: #1 finished! INFO @ Wed, 22 Apr 2020 10:36:40: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:36:40: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:36:40: #2 number of paired peaks: 80 WARNING @ Wed, 22 Apr 2020 10:36:40: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:36:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 10:36:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 10:36:57: #1 read tag files... INFO @ Wed, 22 Apr 2020 10:36:57: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 10:37:03: 1000000 INFO @ Wed, 22 Apr 2020 10:37:08: 2000000 INFO @ Wed, 22 Apr 2020 10:37:09: #1 tag size is determined as 66 bps INFO @ Wed, 22 Apr 2020 10:37:09: #1 tag size = 66 INFO @ Wed, 22 Apr 2020 10:37:09: #1 total tags in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:37:09: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 10:37:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 10:37:09: #1 tags after filtering in treatment: 2203616 INFO @ Wed, 22 Apr 2020 10:37:09: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 10:37:09: #1 finished! INFO @ Wed, 22 Apr 2020 10:37:09: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 10:37:09: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 10:37:09: #2 number of paired peaks: 80 WARNING @ Wed, 22 Apr 2020 10:37:09: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 10:37:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7671077/SRX7671077.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。