Job ID = 14521997 SRX = SRX7637550 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8107782 spots for SRR10972030/SRR10972030.sra Written 8107782 spots for SRR10972030/SRR10972030.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:56 8107782 reads; of these: 8107782 (100.00%) were paired; of these: 350422 (4.32%) aligned concordantly 0 times 6949562 (85.71%) aligned concordantly exactly 1 time 807798 (9.96%) aligned concordantly >1 times ---- 350422 pairs aligned concordantly 0 times; of these: 3645 (1.04%) aligned discordantly 1 time ---- 346777 pairs aligned 0 times concordantly or discordantly; of these: 693554 mates make up the pairs; of these: 631063 (90.99%) aligned 0 times 50105 (7.22%) aligned exactly 1 time 12386 (1.79%) aligned >1 times 96.11% overall alignment rate Time searching: 00:05:56 Overall time: 00:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 988893 / 7759977 = 0.1274 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:08:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:08:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:08:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:07: 1000000 INFO @ Sat, 15 Jan 2022 22:09:14: 2000000 INFO @ Sat, 15 Jan 2022 22:09:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:28: 4000000 INFO @ Sat, 15 Jan 2022 22:09:35: 5000000 INFO @ Sat, 15 Jan 2022 22:09:37: 1000000 INFO @ Sat, 15 Jan 2022 22:09:43: 6000000 INFO @ Sat, 15 Jan 2022 22:09:47: 2000000 INFO @ Sat, 15 Jan 2022 22:09:51: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:56: 3000000 INFO @ Sat, 15 Jan 2022 22:09:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:59: 8000000 INFO @ Sat, 15 Jan 2022 22:10:06: 4000000 INFO @ Sat, 15 Jan 2022 22:10:07: 9000000 INFO @ Sat, 15 Jan 2022 22:10:08: 1000000 INFO @ Sat, 15 Jan 2022 22:10:15: 10000000 INFO @ Sat, 15 Jan 2022 22:10:15: 5000000 INFO @ Sat, 15 Jan 2022 22:10:19: 2000000 INFO @ Sat, 15 Jan 2022 22:10:23: 11000000 INFO @ Sat, 15 Jan 2022 22:10:25: 6000000 INFO @ Sat, 15 Jan 2022 22:10:30: 3000000 INFO @ Sat, 15 Jan 2022 22:10:31: 12000000 INFO @ Sat, 15 Jan 2022 22:10:34: 7000000 INFO @ Sat, 15 Jan 2022 22:10:39: 13000000 INFO @ Sat, 15 Jan 2022 22:10:41: 4000000 INFO @ Sat, 15 Jan 2022 22:10:44: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:10:44: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:10:44: #1 total tags in treatment: 6768852 INFO @ Sat, 15 Jan 2022 22:10:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:10:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:10:44: #1 tags after filtering in treatment: 5357955 INFO @ Sat, 15 Jan 2022 22:10:44: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:10:44: #1 finished! INFO @ Sat, 15 Jan 2022 22:10:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:10:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:10:44: 8000000 INFO @ Sat, 15 Jan 2022 22:10:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:10:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:10:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:10:51: 5000000 INFO @ Sat, 15 Jan 2022 22:10:53: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:11:02: 6000000 INFO @ Sat, 15 Jan 2022 22:11:02: 10000000 INFO @ Sat, 15 Jan 2022 22:11:11: 11000000 INFO @ Sat, 15 Jan 2022 22:11:13: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:11:21: 12000000 INFO @ Sat, 15 Jan 2022 22:11:24: 8000000 INFO @ Sat, 15 Jan 2022 22:11:30: 13000000 INFO @ Sat, 15 Jan 2022 22:11:36: 9000000 INFO @ Sat, 15 Jan 2022 22:11:36: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:11:36: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:11:36: #1 total tags in treatment: 6768852 INFO @ Sat, 15 Jan 2022 22:11:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:11:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:11:36: #1 tags after filtering in treatment: 5357955 INFO @ Sat, 15 Jan 2022 22:11:36: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:11:36: #1 finished! INFO @ Sat, 15 Jan 2022 22:11:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:11:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:11:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:11:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:11:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:11:47: 10000000 INFO @ Sat, 15 Jan 2022 22:11:59: 11000000 INFO @ Sat, 15 Jan 2022 22:12:10: 12000000 INFO @ Sat, 15 Jan 2022 22:12:22: 13000000 INFO @ Sat, 15 Jan 2022 22:12:29: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:12:29: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:12:29: #1 total tags in treatment: 6768852 INFO @ Sat, 15 Jan 2022 22:12:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:12:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:12:29: #1 tags after filtering in treatment: 5357955 INFO @ Sat, 15 Jan 2022 22:12:29: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:12:29: #1 finished! INFO @ Sat, 15 Jan 2022 22:12:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:12:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:12:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:12:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:12:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637550/SRX7637550.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling