Job ID = 14521979 SRX = SRX7637548 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8819461 spots for SRR10972028/SRR10972028.sra Written 8819461 spots for SRR10972028/SRR10972028.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:43 8819461 reads; of these: 8819461 (100.00%) were paired; of these: 566068 (6.42%) aligned concordantly 0 times 7169699 (81.29%) aligned concordantly exactly 1 time 1083694 (12.29%) aligned concordantly >1 times ---- 566068 pairs aligned concordantly 0 times; of these: 99591 (17.59%) aligned discordantly 1 time ---- 466477 pairs aligned 0 times concordantly or discordantly; of these: 932954 mates make up the pairs; of these: 681696 (73.07%) aligned 0 times 161038 (17.26%) aligned exactly 1 time 90220 (9.67%) aligned >1 times 96.14% overall alignment rate Time searching: 00:06:43 Overall time: 00:06:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1672072 / 8331355 = 0.2007 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:08:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:08:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:08:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:08:50: 1000000 INFO @ Sat, 15 Jan 2022 22:08:57: 2000000 INFO @ Sat, 15 Jan 2022 22:09:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:12: 4000000 INFO @ Sat, 15 Jan 2022 22:09:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:12: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:12: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:20: 5000000 INFO @ Sat, 15 Jan 2022 22:09:21: 1000000 INFO @ Sat, 15 Jan 2022 22:09:28: 6000000 INFO @ Sat, 15 Jan 2022 22:09:30: 2000000 INFO @ Sat, 15 Jan 2022 22:09:35: 7000000 INFO @ Sat, 15 Jan 2022 22:09:39: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:43: 8000000 INFO @ Sat, 15 Jan 2022 22:09:48: 4000000 INFO @ Sat, 15 Jan 2022 22:09:51: 9000000 INFO @ Sat, 15 Jan 2022 22:09:52: 1000000 INFO @ Sat, 15 Jan 2022 22:09:57: 5000000 INFO @ Sat, 15 Jan 2022 22:09:59: 10000000 INFO @ Sat, 15 Jan 2022 22:10:01: 2000000 INFO @ Sat, 15 Jan 2022 22:10:07: 6000000 INFO @ Sat, 15 Jan 2022 22:10:08: 11000000 INFO @ Sat, 15 Jan 2022 22:10:09: 3000000 INFO @ Sat, 15 Jan 2022 22:10:16: 12000000 INFO @ Sat, 15 Jan 2022 22:10:17: 7000000 INFO @ Sat, 15 Jan 2022 22:10:18: 4000000 INFO @ Sat, 15 Jan 2022 22:10:24: 13000000 INFO @ Sat, 15 Jan 2022 22:10:26: 8000000 INFO @ Sat, 15 Jan 2022 22:10:27: 5000000 INFO @ Sat, 15 Jan 2022 22:10:30: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:10:30: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:10:30: #1 total tags in treatment: 6584052 INFO @ Sat, 15 Jan 2022 22:10:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:10:30: #1 tags after filtering in treatment: 4972767 INFO @ Sat, 15 Jan 2022 22:10:30: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:10:30: #1 finished! INFO @ Sat, 15 Jan 2022 22:10:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:10:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:10:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:10:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:10:36: 9000000 INFO @ Sat, 15 Jan 2022 22:10:36: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:10:45: 7000000 INFO @ Sat, 15 Jan 2022 22:10:45: 10000000 INFO @ Sat, 15 Jan 2022 22:10:53: 8000000 INFO @ Sat, 15 Jan 2022 22:10:55: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:11:02: 9000000 INFO @ Sat, 15 Jan 2022 22:11:04: 12000000 INFO @ Sat, 15 Jan 2022 22:11:11: 10000000 INFO @ Sat, 15 Jan 2022 22:11:13: 13000000 INFO @ Sat, 15 Jan 2022 22:11:19: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:11:19: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:11:19: #1 total tags in treatment: 6584052 INFO @ Sat, 15 Jan 2022 22:11:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:11:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:11:19: #1 tags after filtering in treatment: 4972767 INFO @ Sat, 15 Jan 2022 22:11:19: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:11:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:11:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:11:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:11:19: 11000000 INFO @ Sat, 15 Jan 2022 22:11:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:11:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:11:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:11:28: 12000000 INFO @ Sat, 15 Jan 2022 22:11:36: 13000000 INFO @ Sat, 15 Jan 2022 22:11:41: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:11:41: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:11:41: #1 total tags in treatment: 6584052 INFO @ Sat, 15 Jan 2022 22:11:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:11:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:11:41: #1 tags after filtering in treatment: 4972767 INFO @ Sat, 15 Jan 2022 22:11:41: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 22:11:41: #1 finished! INFO @ Sat, 15 Jan 2022 22:11:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:11:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:11:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:11:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:11:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637548/SRX7637548.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling