Job ID = 14521977 SRX = SRX7637546 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8050301 spots for SRR10972026/SRR10972026.sra Written 8050301 spots for SRR10972026/SRR10972026.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:54 8050301 reads; of these: 8050301 (100.00%) were paired; of these: 511273 (6.35%) aligned concordantly 0 times 6803572 (84.51%) aligned concordantly exactly 1 time 735456 (9.14%) aligned concordantly >1 times ---- 511273 pairs aligned concordantly 0 times; of these: 3293 (0.64%) aligned discordantly 1 time ---- 507980 pairs aligned 0 times concordantly or discordantly; of these: 1015960 mates make up the pairs; of these: 958658 (94.36%) aligned 0 times 45483 (4.48%) aligned exactly 1 time 11819 (1.16%) aligned >1 times 94.05% overall alignment rate Time searching: 00:05:54 Overall time: 00:05:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 905292 / 7541500 = 0.1200 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:07:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:07:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:07:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:07:14: 1000000 INFO @ Sat, 15 Jan 2022 22:07:21: 2000000 INFO @ Sat, 15 Jan 2022 22:07:28: 3000000 INFO @ Sat, 15 Jan 2022 22:07:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:07:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:07:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:07:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:07:40: 5000000 INFO @ Sat, 15 Jan 2022 22:07:44: 1000000 INFO @ Sat, 15 Jan 2022 22:07:47: 6000000 INFO @ Sat, 15 Jan 2022 22:07:52: 2000000 INFO @ Sat, 15 Jan 2022 22:07:53: 7000000 INFO @ Sat, 15 Jan 2022 22:07:59: 3000000 INFO @ Sat, 15 Jan 2022 22:07:59: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:08:06: 9000000 INFO @ Sat, 15 Jan 2022 22:08:06: 4000000 INFO @ Sat, 15 Jan 2022 22:08:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:08:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:08:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:08:12: 10000000 INFO @ Sat, 15 Jan 2022 22:08:13: 5000000 INFO @ Sat, 15 Jan 2022 22:08:16: 1000000 INFO @ Sat, 15 Jan 2022 22:08:19: 11000000 INFO @ Sat, 15 Jan 2022 22:08:21: 6000000 INFO @ Sat, 15 Jan 2022 22:08:25: 2000000 INFO @ Sat, 15 Jan 2022 22:08:25: 12000000 INFO @ Sat, 15 Jan 2022 22:08:28: 7000000 INFO @ Sat, 15 Jan 2022 22:08:32: 13000000 INFO @ Sat, 15 Jan 2022 22:08:34: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:08:34: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:08:34: #1 total tags in treatment: 6634016 INFO @ Sat, 15 Jan 2022 22:08:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:08:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:08:34: #1 tags after filtering in treatment: 5346211 INFO @ Sat, 15 Jan 2022 22:08:34: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:08:34: #1 finished! INFO @ Sat, 15 Jan 2022 22:08:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:08:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:08:35: 3000000 INFO @ Sat, 15 Jan 2022 22:08:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:08:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:08:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:08:35: 8000000 INFO @ Sat, 15 Jan 2022 22:08:42: 9000000 INFO @ Sat, 15 Jan 2022 22:08:44: 4000000 INFO @ Sat, 15 Jan 2022 22:08:50: 10000000 INFO @ Sat, 15 Jan 2022 22:08:52: 5000000 INFO @ Sat, 15 Jan 2022 22:08:57: 11000000 INFO @ Sat, 15 Jan 2022 22:09:01: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:09:04: 12000000 INFO @ Sat, 15 Jan 2022 22:09:09: 7000000 INFO @ Sat, 15 Jan 2022 22:09:11: 13000000 INFO @ Sat, 15 Jan 2022 22:09:14: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:09:14: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:09:14: #1 total tags in treatment: 6634016 INFO @ Sat, 15 Jan 2022 22:09:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:09:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:09:14: #1 tags after filtering in treatment: 5346211 INFO @ Sat, 15 Jan 2022 22:09:14: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:09:14: #1 finished! INFO @ Sat, 15 Jan 2022 22:09:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:09:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:09:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:09:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:09:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:09:17: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:09:25: 9000000 INFO @ Sat, 15 Jan 2022 22:09:32: 10000000 INFO @ Sat, 15 Jan 2022 22:09:39: 11000000 INFO @ Sat, 15 Jan 2022 22:09:47: 12000000 INFO @ Sat, 15 Jan 2022 22:09:54: 13000000 INFO @ Sat, 15 Jan 2022 22:09:56: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:09:56: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:09:56: #1 total tags in treatment: 6634016 INFO @ Sat, 15 Jan 2022 22:09:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:09:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:09:56: #1 tags after filtering in treatment: 5346211 INFO @ Sat, 15 Jan 2022 22:09:56: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:09:56: #1 finished! INFO @ Sat, 15 Jan 2022 22:09:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:09:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:09:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:09:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:09:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637546/SRX7637546.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling