Job ID = 14521975 SRX = SRX7637544 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11173275 spots for SRR10972024/SRR10972024.sra Written 11173275 spots for SRR10972024/SRR10972024.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:55 11173275 reads; of these: 11173275 (100.00%) were paired; of these: 494500 (4.43%) aligned concordantly 0 times 9232808 (82.63%) aligned concordantly exactly 1 time 1445967 (12.94%) aligned concordantly >1 times ---- 494500 pairs aligned concordantly 0 times; of these: 29638 (5.99%) aligned discordantly 1 time ---- 464862 pairs aligned 0 times concordantly or discordantly; of these: 929724 mates make up the pairs; of these: 795229 (85.53%) aligned 0 times 97225 (10.46%) aligned exactly 1 time 37270 (4.01%) aligned >1 times 96.44% overall alignment rate Time searching: 00:07:55 Overall time: 00:07:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1444972 / 10704344 = 0.1350 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:10:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:10:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:10:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:10:29: 1000000 INFO @ Sat, 15 Jan 2022 22:10:35: 2000000 INFO @ Sat, 15 Jan 2022 22:10:40: 3000000 INFO @ Sat, 15 Jan 2022 22:10:45: 4000000 INFO @ Sat, 15 Jan 2022 22:10:51: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:10:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:10:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:10:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:10:57: 6000000 INFO @ Sat, 15 Jan 2022 22:11:00: 1000000 INFO @ Sat, 15 Jan 2022 22:11:03: 7000000 INFO @ Sat, 15 Jan 2022 22:11:06: 2000000 INFO @ Sat, 15 Jan 2022 22:11:09: 8000000 INFO @ Sat, 15 Jan 2022 22:11:13: 3000000 INFO @ Sat, 15 Jan 2022 22:11:16: 9000000 INFO @ Sat, 15 Jan 2022 22:11:19: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:11:22: 10000000 INFO @ Sat, 15 Jan 2022 22:11:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:11:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:11:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:11:26: 5000000 INFO @ Sat, 15 Jan 2022 22:11:29: 11000000 INFO @ Sat, 15 Jan 2022 22:11:31: 1000000 INFO @ Sat, 15 Jan 2022 22:11:32: 6000000 INFO @ Sat, 15 Jan 2022 22:11:35: 12000000 INFO @ Sat, 15 Jan 2022 22:11:37: 2000000 INFO @ Sat, 15 Jan 2022 22:11:39: 7000000 INFO @ Sat, 15 Jan 2022 22:11:42: 13000000 INFO @ Sat, 15 Jan 2022 22:11:44: 3000000 INFO @ Sat, 15 Jan 2022 22:11:45: 8000000 INFO @ Sat, 15 Jan 2022 22:11:48: 14000000 INFO @ Sat, 15 Jan 2022 22:11:51: 4000000 INFO @ Sat, 15 Jan 2022 22:11:52: 9000000 INFO @ Sat, 15 Jan 2022 22:11:55: 15000000 INFO @ Sat, 15 Jan 2022 22:11:57: 5000000 INFO @ Sat, 15 Jan 2022 22:11:59: 10000000 INFO @ Sat, 15 Jan 2022 22:12:01: 16000000 INFO @ Sat, 15 Jan 2022 22:12:04: 6000000 INFO @ Sat, 15 Jan 2022 22:12:05: 11000000 INFO @ Sat, 15 Jan 2022 22:12:08: 17000000 INFO @ Sat, 15 Jan 2022 22:12:11: 7000000 INFO @ Sat, 15 Jan 2022 22:12:12: 12000000 INFO @ Sat, 15 Jan 2022 22:12:15: 18000000 INFO @ Sat, 15 Jan 2022 22:12:18: 8000000 INFO @ Sat, 15 Jan 2022 22:12:19: 13000000 INFO @ Sat, 15 Jan 2022 22:12:19: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:12:19: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:12:19: #1 total tags in treatment: 9235425 INFO @ Sat, 15 Jan 2022 22:12:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:12:19: #1 tags after filtering in treatment: 6643506 INFO @ Sat, 15 Jan 2022 22:12:19: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:12:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:12:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:12:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:12:20: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:12:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:12:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:12:24: 9000000 INFO @ Sat, 15 Jan 2022 22:12:26: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:12:31: 10000000 INFO @ Sat, 15 Jan 2022 22:12:32: 15000000 INFO @ Sat, 15 Jan 2022 22:12:37: 11000000 INFO @ Sat, 15 Jan 2022 22:12:38: 16000000 INFO @ Sat, 15 Jan 2022 22:12:43: 12000000 INFO @ Sat, 15 Jan 2022 22:12:44: 17000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:12:49: 13000000 INFO @ Sat, 15 Jan 2022 22:12:49: 18000000 INFO @ Sat, 15 Jan 2022 22:12:53: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:12:53: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:12:53: #1 total tags in treatment: 9235425 INFO @ Sat, 15 Jan 2022 22:12:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:12:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:12:53: #1 tags after filtering in treatment: 6643506 INFO @ Sat, 15 Jan 2022 22:12:53: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:12:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:12:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:12:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:12:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:12:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:12:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:12:55: 14000000 INFO @ Sat, 15 Jan 2022 22:13:01: 15000000 INFO @ Sat, 15 Jan 2022 22:13:06: 16000000 INFO @ Sat, 15 Jan 2022 22:13:12: 17000000 INFO @ Sat, 15 Jan 2022 22:13:18: 18000000 INFO @ Sat, 15 Jan 2022 22:13:21: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:13:21: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:13:21: #1 total tags in treatment: 9235425 INFO @ Sat, 15 Jan 2022 22:13:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:13:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:13:22: #1 tags after filtering in treatment: 6643506 INFO @ Sat, 15 Jan 2022 22:13:22: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:13:22: #1 finished! INFO @ Sat, 15 Jan 2022 22:13:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:13:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:13:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:13:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:13:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637544/SRX7637544.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling