Job ID = 14521959 SRX = SRX7637538 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7553832 spots for SRR10972018/SRR10972018.sra Written 7553832 spots for SRR10972018/SRR10972018.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:55 7553832 reads; of these: 7553832 (100.00%) were paired; of these: 309584 (4.10%) aligned concordantly 0 times 6534281 (86.50%) aligned concordantly exactly 1 time 709967 (9.40%) aligned concordantly >1 times ---- 309584 pairs aligned concordantly 0 times; of these: 5491 (1.77%) aligned discordantly 1 time ---- 304093 pairs aligned 0 times concordantly or discordantly; of these: 608186 mates make up the pairs; of these: 547182 (89.97%) aligned 0 times 48712 (8.01%) aligned exactly 1 time 12292 (2.02%) aligned >1 times 96.38% overall alignment rate Time searching: 00:03:55 Overall time: 00:03:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 630858 / 7247717 = 0.0870 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:01:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:01:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:01:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:01:34: 1000000 INFO @ Sat, 15 Jan 2022 22:01:39: 2000000 INFO @ Sat, 15 Jan 2022 22:01:44: 3000000 INFO @ Sat, 15 Jan 2022 22:01:48: 4000000 INFO @ Sat, 15 Jan 2022 22:01:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:01:58: 6000000 INFO @ Sat, 15 Jan 2022 22:02:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:03: 7000000 INFO @ Sat, 15 Jan 2022 22:02:05: 1000000 INFO @ Sat, 15 Jan 2022 22:02:08: 8000000 INFO @ Sat, 15 Jan 2022 22:02:10: 2000000 INFO @ Sat, 15 Jan 2022 22:02:14: 9000000 INFO @ Sat, 15 Jan 2022 22:02:15: 3000000 INFO @ Sat, 15 Jan 2022 22:02:19: 10000000 INFO @ Sat, 15 Jan 2022 22:02:20: 4000000 INFO @ Sat, 15 Jan 2022 22:02:25: 5000000 INFO @ Sat, 15 Jan 2022 22:02:25: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:29: 6000000 INFO @ Sat, 15 Jan 2022 22:02:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:31: 12000000 INFO @ Sat, 15 Jan 2022 22:02:34: 7000000 INFO @ Sat, 15 Jan 2022 22:02:35: 1000000 INFO @ Sat, 15 Jan 2022 22:02:36: 13000000 INFO @ Sat, 15 Jan 2022 22:02:37: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:02:37: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:02:37: #1 total tags in treatment: 6613822 INFO @ Sat, 15 Jan 2022 22:02:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:02:37: #1 tags after filtering in treatment: 5253333 INFO @ Sat, 15 Jan 2022 22:02:37: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:02:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:02:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:02:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:02:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:02:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:02:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:02:40: 8000000 INFO @ Sat, 15 Jan 2022 22:02:41: 2000000 INFO @ Sat, 15 Jan 2022 22:02:45: 9000000 INFO @ Sat, 15 Jan 2022 22:02:47: 3000000 INFO @ Sat, 15 Jan 2022 22:02:50: 10000000 INFO @ Sat, 15 Jan 2022 22:02:52: 4000000 INFO @ Sat, 15 Jan 2022 22:02:55: 11000000 INFO @ Sat, 15 Jan 2022 22:02:58: 5000000 INFO @ Sat, 15 Jan 2022 22:03:01: 12000000 INFO @ Sat, 15 Jan 2022 22:03:03: 6000000 INFO @ Sat, 15 Jan 2022 22:03:06: 13000000 INFO @ Sat, 15 Jan 2022 22:03:07: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:03:07: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:03:07: #1 total tags in treatment: 6613822 INFO @ Sat, 15 Jan 2022 22:03:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:07: #1 tags after filtering in treatment: 5253333 INFO @ Sat, 15 Jan 2022 22:03:07: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:03:07: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:09: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:03:14: 8000000 INFO @ Sat, 15 Jan 2022 22:03:19: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:03:25: 10000000 INFO @ Sat, 15 Jan 2022 22:03:30: 11000000 INFO @ Sat, 15 Jan 2022 22:03:35: 12000000 INFO @ Sat, 15 Jan 2022 22:03:41: 13000000 INFO @ Sat, 15 Jan 2022 22:03:42: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:03:42: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:03:42: #1 total tags in treatment: 6613822 INFO @ Sat, 15 Jan 2022 22:03:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:42: #1 tags after filtering in treatment: 5253333 INFO @ Sat, 15 Jan 2022 22:03:42: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:03:42: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637538/SRX7637538.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling