Job ID = 14521958 SRX = SRX7637537 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 1849463 spots for SRR10972017/SRR10972017.sra Written 1849463 spots for SRR10972017/SRR10972017.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 1849463 reads; of these: 1849463 (100.00%) were paired; of these: 511414 (27.65%) aligned concordantly 0 times 583221 (31.53%) aligned concordantly exactly 1 time 754828 (40.81%) aligned concordantly >1 times ---- 511414 pairs aligned concordantly 0 times; of these: 842 (0.16%) aligned discordantly 1 time ---- 510572 pairs aligned 0 times concordantly or discordantly; of these: 1021144 mates make up the pairs; of these: 1011133 (99.02%) aligned 0 times 5621 (0.55%) aligned exactly 1 time 4390 (0.43%) aligned >1 times 72.66% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 292851 / 1336479 = 0.2191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:58:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:58:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:58:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:58:39: 1000000 INFO @ Sat, 15 Jan 2022 21:58:48: 2000000 INFO @ Sat, 15 Jan 2022 21:58:49: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 21:58:49: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 21:58:49: #1 total tags in treatment: 1045208 INFO @ Sat, 15 Jan 2022 21:58:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:58:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:58:49: #1 tags after filtering in treatment: 705516 INFO @ Sat, 15 Jan 2022 21:58:49: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 21:58:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:58:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:58:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:58:49: #2 number of paired peaks: 81 WARNING @ Sat, 15 Jan 2022 21:58:49: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:58:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:59:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:59:01: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:59:01: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:59:09: 1000000 INFO @ Sat, 15 Jan 2022 21:59:18: 2000000 INFO @ Sat, 15 Jan 2022 21:59:19: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 21:59:19: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 21:59:19: #1 total tags in treatment: 1045208 INFO @ Sat, 15 Jan 2022 21:59:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:59:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:59:19: #1 tags after filtering in treatment: 705516 INFO @ Sat, 15 Jan 2022 21:59:19: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 21:59:19: #1 finished! INFO @ Sat, 15 Jan 2022 21:59:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:59:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:59:19: #2 number of paired peaks: 81 WARNING @ Sat, 15 Jan 2022 21:59:19: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:59:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:59:31: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:59:31: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:59:39: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:59:48: 2000000 INFO @ Sat, 15 Jan 2022 21:59:49: #1 tag size is determined as 74 bps INFO @ Sat, 15 Jan 2022 21:59:49: #1 tag size = 74 INFO @ Sat, 15 Jan 2022 21:59:49: #1 total tags in treatment: 1045208 INFO @ Sat, 15 Jan 2022 21:59:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:59:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:59:49: #1 tags after filtering in treatment: 705516 INFO @ Sat, 15 Jan 2022 21:59:49: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 21:59:49: #1 finished! INFO @ Sat, 15 Jan 2022 21:59:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:59:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:59:49: #2 number of paired peaks: 81 WARNING @ Sat, 15 Jan 2022 21:59:49: Too few paired peaks (81) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:59:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637537/SRX7637537.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling