Job ID = 14521946 SRX = SRX7637528 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9824662 spots for SRR10972040/SRR10972040.sra Written 9824662 spots for SRR10972040/SRR10972040.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:46 9824662 reads; of these: 9824662 (100.00%) were paired; of these: 482612 (4.91%) aligned concordantly 0 times 8262193 (84.10%) aligned concordantly exactly 1 time 1079857 (10.99%) aligned concordantly >1 times ---- 482612 pairs aligned concordantly 0 times; of these: 7558 (1.57%) aligned discordantly 1 time ---- 475054 pairs aligned 0 times concordantly or discordantly; of these: 950108 mates make up the pairs; of these: 811709 (85.43%) aligned 0 times 109991 (11.58%) aligned exactly 1 time 28408 (2.99%) aligned >1 times 95.87% overall alignment rate Time searching: 00:04:46 Overall time: 00:04:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1808157 / 9348907 = 0.1934 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:21: 1000000 INFO @ Sat, 15 Jan 2022 22:02:25: 2000000 INFO @ Sat, 15 Jan 2022 22:02:28: 3000000 INFO @ Sat, 15 Jan 2022 22:02:32: 4000000 INFO @ Sat, 15 Jan 2022 22:02:36: 5000000 INFO @ Sat, 15 Jan 2022 22:02:40: 6000000 INFO @ Sat, 15 Jan 2022 22:02:44: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:02:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:02:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:02:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:02:48: 8000000 INFO @ Sat, 15 Jan 2022 22:02:51: 1000000 INFO @ Sat, 15 Jan 2022 22:02:52: 9000000 INFO @ Sat, 15 Jan 2022 22:02:55: 2000000 INFO @ Sat, 15 Jan 2022 22:02:57: 10000000 INFO @ Sat, 15 Jan 2022 22:02:59: 3000000 INFO @ Sat, 15 Jan 2022 22:03:01: 11000000 INFO @ Sat, 15 Jan 2022 22:03:03: 4000000 INFO @ Sat, 15 Jan 2022 22:03:05: 12000000 INFO @ Sat, 15 Jan 2022 22:03:07: 5000000 INFO @ Sat, 15 Jan 2022 22:03:09: 13000000 INFO @ Sat, 15 Jan 2022 22:03:12: 6000000 INFO @ Sat, 15 Jan 2022 22:03:13: 14000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:03:16: 7000000 INFO @ Sat, 15 Jan 2022 22:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:03:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:03:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:03:18: 15000000 INFO @ Sat, 15 Jan 2022 22:03:19: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:03:19: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:03:19: #1 total tags in treatment: 7535050 INFO @ Sat, 15 Jan 2022 22:03:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:19: #1 tags after filtering in treatment: 5264334 INFO @ Sat, 15 Jan 2022 22:03:19: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:03:19: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:20: 8000000 INFO @ Sat, 15 Jan 2022 22:03:21: 1000000 INFO @ Sat, 15 Jan 2022 22:03:24: 9000000 INFO @ Sat, 15 Jan 2022 22:03:25: 2000000 INFO @ Sat, 15 Jan 2022 22:03:28: 10000000 INFO @ Sat, 15 Jan 2022 22:03:29: 3000000 INFO @ Sat, 15 Jan 2022 22:03:32: 11000000 INFO @ Sat, 15 Jan 2022 22:03:34: 4000000 INFO @ Sat, 15 Jan 2022 22:03:37: 12000000 INFO @ Sat, 15 Jan 2022 22:03:38: 5000000 INFO @ Sat, 15 Jan 2022 22:03:41: 13000000 INFO @ Sat, 15 Jan 2022 22:03:42: 6000000 INFO @ Sat, 15 Jan 2022 22:03:45: 14000000 INFO @ Sat, 15 Jan 2022 22:03:46: 7000000 INFO @ Sat, 15 Jan 2022 22:03:49: 15000000 INFO @ Sat, 15 Jan 2022 22:03:50: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:03:50: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:03:50: #1 total tags in treatment: 7535050 INFO @ Sat, 15 Jan 2022 22:03:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:03:50: #1 tags after filtering in treatment: 5264334 INFO @ Sat, 15 Jan 2022 22:03:50: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:03:50: #1 finished! INFO @ Sat, 15 Jan 2022 22:03:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:03:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:03:50: 8000000 INFO @ Sat, 15 Jan 2022 22:03:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:03:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:03:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:03:55: 9000000 INFO @ Sat, 15 Jan 2022 22:03:59: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:04:03: 11000000 INFO @ Sat, 15 Jan 2022 22:04:07: 12000000 INFO @ Sat, 15 Jan 2022 22:04:11: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:04:15: 14000000 INFO @ Sat, 15 Jan 2022 22:04:19: 15000000 INFO @ Sat, 15 Jan 2022 22:04:20: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:04:20: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:04:20: #1 total tags in treatment: 7535050 INFO @ Sat, 15 Jan 2022 22:04:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:04:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:04:20: #1 tags after filtering in treatment: 5264334 INFO @ Sat, 15 Jan 2022 22:04:20: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 22:04:20: #1 finished! INFO @ Sat, 15 Jan 2022 22:04:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:04:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:04:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:04:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:04:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637528/SRX7637528.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling