Job ID = 14521944 SRX = SRX7637526 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8002080 spots for SRR10972038/SRR10972038.sra Written 8002080 spots for SRR10972038/SRR10972038.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:50 8002080 reads; of these: 8002080 (100.00%) were paired; of these: 412261 (5.15%) aligned concordantly 0 times 6858748 (85.71%) aligned concordantly exactly 1 time 731071 (9.14%) aligned concordantly >1 times ---- 412261 pairs aligned concordantly 0 times; of these: 3988 (0.97%) aligned discordantly 1 time ---- 408273 pairs aligned 0 times concordantly or discordantly; of these: 816546 mates make up the pairs; of these: 757589 (92.78%) aligned 0 times 47564 (5.83%) aligned exactly 1 time 11393 (1.40%) aligned >1 times 95.27% overall alignment rate Time searching: 00:03:50 Overall time: 00:03:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 965081 / 7592729 = 0.1271 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:00:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:00:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:00:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:00:05: 1000000 INFO @ Sat, 15 Jan 2022 22:00:11: 2000000 INFO @ Sat, 15 Jan 2022 22:00:16: 3000000 INFO @ Sat, 15 Jan 2022 22:00:22: 4000000 INFO @ Sat, 15 Jan 2022 22:00:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:00:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:00:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:00:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:00:33: 6000000 INFO @ Sat, 15 Jan 2022 22:00:35: 1000000 INFO @ Sat, 15 Jan 2022 22:00:39: 7000000 INFO @ Sat, 15 Jan 2022 22:00:40: 2000000 INFO @ Sat, 15 Jan 2022 22:00:45: 8000000 INFO @ Sat, 15 Jan 2022 22:00:45: 3000000 INFO @ Sat, 15 Jan 2022 22:00:50: 4000000 INFO @ Sat, 15 Jan 2022 22:00:51: 9000000 INFO @ Sat, 15 Jan 2022 22:00:55: 5000000 INFO @ Sat, 15 Jan 2022 22:00:56: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:01:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:01:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:01:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:01:00: 6000000 INFO @ Sat, 15 Jan 2022 22:01:02: 11000000 INFO @ Sat, 15 Jan 2022 22:01:05: 7000000 INFO @ Sat, 15 Jan 2022 22:01:06: 1000000 INFO @ Sat, 15 Jan 2022 22:01:08: 12000000 INFO @ Sat, 15 Jan 2022 22:01:10: 8000000 INFO @ Sat, 15 Jan 2022 22:01:11: 2000000 INFO @ Sat, 15 Jan 2022 22:01:14: 13000000 INFO @ Sat, 15 Jan 2022 22:01:15: 9000000 INFO @ Sat, 15 Jan 2022 22:01:16: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:01:16: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:01:16: #1 total tags in treatment: 6625239 INFO @ Sat, 15 Jan 2022 22:01:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:16: #1 tags after filtering in treatment: 5351058 INFO @ Sat, 15 Jan 2022 22:01:16: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:01:16: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:01:17: 3000000 INFO @ Sat, 15 Jan 2022 22:01:20: 10000000 INFO @ Sat, 15 Jan 2022 22:01:23: 4000000 INFO @ Sat, 15 Jan 2022 22:01:25: 11000000 INFO @ Sat, 15 Jan 2022 22:01:28: 5000000 INFO @ Sat, 15 Jan 2022 22:01:30: 12000000 INFO @ Sat, 15 Jan 2022 22:01:34: 6000000 INFO @ Sat, 15 Jan 2022 22:01:35: 13000000 INFO @ Sat, 15 Jan 2022 22:01:37: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:01:37: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:01:37: #1 total tags in treatment: 6625239 INFO @ Sat, 15 Jan 2022 22:01:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:01:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:01:37: #1 tags after filtering in treatment: 5351058 INFO @ Sat, 15 Jan 2022 22:01:37: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:01:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:01:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:01:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:01:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:01:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:01:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:01:40: 7000000 INFO @ Sat, 15 Jan 2022 22:01:46: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:01:51: 9000000 INFO @ Sat, 15 Jan 2022 22:01:57: 10000000 INFO @ Sat, 15 Jan 2022 22:02:02: 11000000 INFO @ Sat, 15 Jan 2022 22:02:08: 12000000 INFO @ Sat, 15 Jan 2022 22:02:13: 13000000 INFO @ Sat, 15 Jan 2022 22:02:15: #1 tag size is determined as 41 bps INFO @ Sat, 15 Jan 2022 22:02:15: #1 tag size = 41 INFO @ Sat, 15 Jan 2022 22:02:15: #1 total tags in treatment: 6625239 INFO @ Sat, 15 Jan 2022 22:02:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:02:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:02:15: #1 tags after filtering in treatment: 5351058 INFO @ Sat, 15 Jan 2022 22:02:15: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 15 Jan 2022 22:02:15: #1 finished! INFO @ Sat, 15 Jan 2022 22:02:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:02:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:02:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:02:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:02:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637526/SRX7637526.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling