Job ID = 14521942 SRX = SRX7637524 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12887644 spots for SRR10972036/SRR10972036.sra Written 12887644 spots for SRR10972036/SRR10972036.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:09:05 12887644 reads; of these: 12887644 (100.00%) were paired; of these: 649530 (5.04%) aligned concordantly 0 times 10958540 (85.03%) aligned concordantly exactly 1 time 1279574 (9.93%) aligned concordantly >1 times ---- 649530 pairs aligned concordantly 0 times; of these: 9045 (1.39%) aligned discordantly 1 time ---- 640485 pairs aligned 0 times concordantly or discordantly; of these: 1280970 mates make up the pairs; of these: 1136342 (88.71%) aligned 0 times 116699 (9.11%) aligned exactly 1 time 27929 (2.18%) aligned >1 times 95.59% overall alignment rate Time searching: 00:09:06 Overall time: 00:09:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3435661 / 12246328 = 0.2805 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:08:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:08:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:08:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:08:58: 1000000 INFO @ Sat, 15 Jan 2022 22:09:06: 2000000 INFO @ Sat, 15 Jan 2022 22:09:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:20: 4000000 INFO @ Sat, 15 Jan 2022 22:09:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:27: 5000000 INFO @ Sat, 15 Jan 2022 22:09:28: 1000000 INFO @ Sat, 15 Jan 2022 22:09:34: 6000000 INFO @ Sat, 15 Jan 2022 22:09:35: 2000000 INFO @ Sat, 15 Jan 2022 22:09:40: 7000000 INFO @ Sat, 15 Jan 2022 22:09:42: 3000000 INFO @ Sat, 15 Jan 2022 22:09:47: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:09:49: 4000000 INFO @ Sat, 15 Jan 2022 22:09:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:09:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:09:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:09:54: 9000000 INFO @ Sat, 15 Jan 2022 22:09:56: 5000000 INFO @ Sat, 15 Jan 2022 22:09:58: 1000000 INFO @ Sat, 15 Jan 2022 22:10:01: 10000000 INFO @ Sat, 15 Jan 2022 22:10:03: 6000000 INFO @ Sat, 15 Jan 2022 22:10:05: 2000000 INFO @ Sat, 15 Jan 2022 22:10:08: 11000000 INFO @ Sat, 15 Jan 2022 22:10:10: 7000000 INFO @ Sat, 15 Jan 2022 22:10:12: 3000000 INFO @ Sat, 15 Jan 2022 22:10:15: 12000000 INFO @ Sat, 15 Jan 2022 22:10:17: 8000000 INFO @ Sat, 15 Jan 2022 22:10:19: 4000000 INFO @ Sat, 15 Jan 2022 22:10:22: 13000000 INFO @ Sat, 15 Jan 2022 22:10:24: 9000000 INFO @ Sat, 15 Jan 2022 22:10:26: 5000000 INFO @ Sat, 15 Jan 2022 22:10:29: 14000000 INFO @ Sat, 15 Jan 2022 22:10:31: 10000000 INFO @ Sat, 15 Jan 2022 22:10:33: 6000000 INFO @ Sat, 15 Jan 2022 22:10:35: 15000000 INFO @ Sat, 15 Jan 2022 22:10:38: 11000000 INFO @ Sat, 15 Jan 2022 22:10:40: 7000000 INFO @ Sat, 15 Jan 2022 22:10:42: 16000000 INFO @ Sat, 15 Jan 2022 22:10:45: 12000000 INFO @ Sat, 15 Jan 2022 22:10:47: 8000000 INFO @ Sat, 15 Jan 2022 22:10:49: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:10:52: 13000000 INFO @ Sat, 15 Jan 2022 22:10:53: 9000000 INFO @ Sat, 15 Jan 2022 22:10:55: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:10:55: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:10:55: #1 total tags in treatment: 8804528 INFO @ Sat, 15 Jan 2022 22:10:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:10:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:10:55: #1 tags after filtering in treatment: 6312522 INFO @ Sat, 15 Jan 2022 22:10:55: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:10:55: #1 finished! INFO @ Sat, 15 Jan 2022 22:10:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:10:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:10:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:10:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:10:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:10:58: 14000000 INFO @ Sat, 15 Jan 2022 22:11:00: 10000000 INFO @ Sat, 15 Jan 2022 22:11:05: 15000000 INFO @ Sat, 15 Jan 2022 22:11:07: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:11:12: 16000000 INFO @ Sat, 15 Jan 2022 22:11:14: 12000000 INFO @ Sat, 15 Jan 2022 22:11:19: 17000000 INFO @ Sat, 15 Jan 2022 22:11:21: 13000000 INFO @ Sat, 15 Jan 2022 22:11:24: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:11:24: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:11:24: #1 total tags in treatment: 8804528 INFO @ Sat, 15 Jan 2022 22:11:24: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:11:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:11:24: #1 tags after filtering in treatment: 6312522 INFO @ Sat, 15 Jan 2022 22:11:24: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:11:24: #1 finished! INFO @ Sat, 15 Jan 2022 22:11:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:11:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:11:25: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:11:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:11:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:11:28: 14000000 INFO @ Sat, 15 Jan 2022 22:11:34: 15000000 INFO @ Sat, 15 Jan 2022 22:11:41: 16000000 INFO @ Sat, 15 Jan 2022 22:11:48: 17000000 INFO @ Sat, 15 Jan 2022 22:11:53: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:11:53: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:11:53: #1 total tags in treatment: 8804528 INFO @ Sat, 15 Jan 2022 22:11:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:11:53: #1 tags after filtering in treatment: 6312522 INFO @ Sat, 15 Jan 2022 22:11:53: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 22:11:53: #1 finished! INFO @ Sat, 15 Jan 2022 22:11:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:11:53: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 22:11:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:11:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7637524/SRX7637524.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling